Volume 19 Issue 11
Nov.  2021
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Article Navigation >  Chinese Journal of General Practice  > 2021  >  19(11): 1807-1810,1900
CAI Xin, LI Yan-yu, SU Guo-mei, LYU Qiu-rong, LAI Tian-wen. Effects of Annexin A1 on inflammatory response and collagen deposition of lung fibroblast[J]. Chinese Journal of General Practice, 2021, 19(11): 1807-1810,1900. doi: 10.16766/j.cnki.issn.1674-4152.002172
Citation: CAI Xin, LI Yan-yu, SU Guo-mei, LYU Qiu-rong, LAI Tian-wen. Effects of Annexin A1 on inflammatory response and collagen deposition of lung fibroblast[J]. Chinese Journal of General Practice, 2021, 19(11): 1807-1810,1900. doi: 10.16766/j.cnki.issn.1674-4152.002172
shu

Effects of Annexin A1 on inflammatory response and collagen deposition of lung fibroblast

doi: 10.16766/j.cnki.issn.1674-4152.002172

  • Department of Respiratory and Critical Care Medicine, the Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, China

Funds:

 A2018162

  • Received Date: 2020-12-25
    Available Online: 2022-02-15
    Abstract
  •   Objective  To investigate the effects of Annexin A1 on inflammatory response, proliferation and collagen deposition of pulmonary fibroblasts.   Methods  The primary lung fibroblasts of C57/BL6 mice were isolated. After 24 or 48 hours of intervention with different concentrations of Annexin A1, the expression of inflammatory factors, cell proliferation and collagen deposition were detected. C57/BL6 mice were randomly divided into control group and Annexin A1 intervention group according to the random number table method, with 6-8 mice in each group. Each mouse in the Annexin A1 intervention group was instilled with mouse recombinant Annexin A1 (1 μg/50 μL) into the trachea, and each mouse in the control group was instilled with 50 μL saline. After continuous intervention for 7 days, mice were sacrificed for measurement. Inflammatory factors, collagen Ⅲ and airway smooth muscle actin (α-SMA) were detected.   Results  Inflammatory cytokines (IL-6, IL-8), proliferation, collagen Ⅲ, and α-SMA were significantly increased in the Annexin A1 intervention group compared with the control group (all P < 0.05). Compared with control mice, the total number of cells in the alveolar lavage fluid of the Annexin A1 intervention group [(2.91±0.26)×104/mL vs. (7.03±0.48)×104 /mL, t=7.432, P=0.008] and the number of neutrophils [(0.12±0.04)×104/mL vs. (1.01±0.05)×104/mL, t=13.810, P < 0.001] were significantly increased, and the expression of inflammatory cytokines, collagen deposition, and smooth muscle were also significantly increased (all P < 0.05).   Conclusion  Annexin A1 induces inflammation, proliferation and collagen deposition in the lung fibroblasts, suggesting that Annexin A1 maybe involved in the pathogenesis of airway remodeling.

     

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