Volume 19 Issue 11
Nov.  2021
Turn off MathJax
Article Contents
Article Navigation >  Chinese Journal of General Practice  > 2021  >  19(11): 1831-1834
HAN Hong-liang, HONG Dao-xian, CHEN Ying, SONG Jun-jie, HE Dong-hai, LI Hong-ying, JIA Qiu-fang. Inhibition of trigeminal ganglion satellite glial cell inflammation by isohalothane regulating the NF-κB/iNOS-COX2 signalling pathway[J]. Chinese Journal of General Practice, 2021, 19(11): 1831-1834. doi: 10.16766/j.cnki.issn.1674-4152.002178
Citation: HAN Hong-liang, HONG Dao-xian, CHEN Ying, SONG Jun-jie, HE Dong-hai, LI Hong-ying, JIA Qiu-fang. Inhibition of trigeminal ganglion satellite glial cell inflammation by isohalothane regulating the NF-κB/iNOS-COX2 signalling pathway[J]. Chinese Journal of General Practice, 2021, 19(11): 1831-1834. doi: 10.16766/j.cnki.issn.1674-4152.002178
shu

Inhibition of trigeminal ganglion satellite glial cell inflammation by isohalothane regulating the NF-κB/iNOS-COX2 signalling pathway

doi: 10.16766/j.cnki.issn.1674-4152.002178
  • 1.

    Department of Anesthesiology, the First Affiliated Hospital of Henan University, Kaifeng, Henan 475000, China

Funds:

 18B310010

  • Received Date: 2020-05-15
    Available Online: 2022-02-15
    Abstract
  •   Objective  To investigate the effect of isohalothane (ISO) on rat trigeminal ganglion satellite glial cell inflammation in vitro and its mechanism.   Methods  Rat trigeminal ganglion satellite glial cells were cultured in vitro. Methyl thiazolyl tetrazolium and lactate dehydrogenase were used to select the appropriate ISO concentration. Cells were divided into the control group, model group, ISO group and SN50 group [nuclear factor (NF)-κB depressor] group. Nitroglycerin (GTN) was used to construct a satellite glial inflammatory model. The ISO group was treated with 1.5% ISO at a rate of 2 L/min for 30 min, and the other groups were treated with air at the same rate for 30 min. The levels of interleukin-1β (IL-1β), tumour necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2) and the content of NO in the cell culture medium were measured by enzyme-linked immunosorbent assay and nitric oxide (NO) assay. The protein expressions of NF-κB p65, cyclooxygenase-2 (COX2) and inducible nitric oxide synthase (iNOS) were detected by western blot. The concentration of calcium ions was detected by Fluo-3/AM probe loading method.   Results  The levels of IL-1β, TNF-α, PGE2 and NO and calcium ion concentration in the ISO group were (121.49±12.37) ng/L, (88.41±9.53) ng/L, (439.92±28.81) pg/mL, (9.03±1.04) μmol/L and (101.11±8.23) mmol/L, respectively, which were significantly lower than those in the model group (all P < 0.05). The results of western blotting analysis showed that the protein expression levels of NF-κB p65, COX2 and iNOS in the ISO group were 0.74±0.15, 0.59±0.14 and 0.51±0.11, respectively, which were significantly lower than those in the model group (all P < 0.05). The changing trend of each index in the SN50 group was consistent with that in the ISO group.   Conclusion  Appropriate dose of ISO can inhibit GTN induced inflammation of trigeminal ganglion satellite glial cells, and the mechanism may be related to reducing the release of inflammatory factors, inhibiting NF- κB/inos-COX2 signalling pathway and the decreasing the calcium concentration.

     

    • FullText(HTML)
  • loading
    • References(21)
  • [1]
    张月战, 徐峰. 偏头痛共病的发病机制研究进展[J]. 中国全科医学, 2017, 20(3): 360-364. doi: 10.3969/j.issn.1007-9572.2017.03.018
    [2]
    张帆. 近十年偏头痛与三叉神经血管疼痛通路解剖研究进展[J]. 中国疼痛医学杂志, 2014, 20(12): 889-891. doi: 10.3969/j.issn.1006-9852.2014.12.011
    [3]
    范红珍, 姚文涛, 吕眯眯, 等. 偏头痛实验模型研究进展[J]. 中国疼痛医学杂志, 2017, 23(11): 841-845. https://www.cnki.com.cn/Article/CJFDTOTAL-ZTYZ201711011.htm
    [4]
    李琴瑶, 杨红军. 偏头痛相关炎性因子的研究进展[J]. 中国疼痛医学杂志, 2018, 24(6): 456-459. doi: 10.3969/j.issn.1006-9852.2018.06.012
    [5]
    WANG H, WANG L, LI N L, et al. Subanesthetic isoflurane reduces zymosan-induced inflammation in murine Kupffer cells by inhibiting ROS-activated p38 MAPK/NF-κB signaling[J]. Oxid Med Cell Longev, 2014: 851692. DOI: 10.1155/2014/851692.
    [6]
    杨毅, 韩晨阳, 郭丽, 等. 米诺环素抑制卫星胶质细胞激活对于三叉神经痛大鼠模型镇痛机制的研究[J]. 中华神经医学杂志, 2018, 17(7): 656-661. doi: 10.3760/cma.j.issn.1671-8925.2018.07.002
    [7]
    MARONE I M, DE LOGU F, NASSINI R, et al. TRPA1/NOX in the soma of trigeminal ganglion neurons mediates migraine-related pain of glyceryl trinitrate in mice[J]. Brain, 2018, 141(8): 2312-2328. doi: 10.1093/brain/awy177
    [8]
    FARKAS S, BÖLCSKEI K, MARKOVICS A, et al. Utility of different outcome measures for the nitroglycerin model of migraine in mice[J]. J Pharmacol Toxicol Methods, 2016, 77: 33-44. doi: 10.1016/j.vascn.2015.09.006
    [9]
    郭宇鹏, 张婵娟, 王晓松, 等. 米诺环素对三叉神经痛大鼠三叉神经节卫星胶质细胞炎症反应的影响[J]. 临床和实验医学杂志, 2019, 18(9): 901-905. https://www.cnki.com.cn/Article/CJFDTOTAL-SYLC201909002.htm
    [10]
    DONG X, GUAN X, CHEN K, et al. Abnormal mitochondrial dynamics and impaired mitochondrial biogenesis in trigeminal ganglion neurons in a rat model of migraine[J]. Neurosci Lett, 2017, 636: 127-133. doi: 10.1016/j.neulet.2016.10.054
    [11]
    LIU H, ZHAO L, GU W, et al. Activation of satellite glial cells in trigeminal ganglion following dental injury and inflammation[J]. J Mol Histol, 2018, 49(3): 257-263. doi: 10.1007/s10735-018-9765-4
    [12]
    吕慧敏, 赵璞, 李新峰, 等. 异氟烷对大鼠脑外伤后继发性损伤的保护作用及其机制[J]. 中华实验外科杂志, 2015, 32(11): 2731-2734.
    [13]
    ZHANG J, GU Z Q, XUE L, et al. Anesthetic isoflurane reduces neuropathic pain by inhibiting IL-1β-induced COX-2 activation and CCL2 release in rat trigeminal ganglia cells[J]. Int J Clin Exp Med, 2016, 9(6): 10229-10237. http://www.researchgate.net/publication/305373913_Anesthetic_isoflurane_reduces_neuropathic_pain_by_inhibiting_IL-1b-induced_COX-2_activation_and_CCL2_release_in_rat_trigeminal_ganglia_cells
    [14]
    陈静, 利莉, 梁羽冰, 等. 异丙酚对胎鼠离体海马神经元钙离子浓度和NF-κB活性的影响[J]. 中华麻醉学杂志, 2014, 34(3): 286-289.
    [15]
    LI Y, ZHANG Q, QI D, et al. Valproate ameliorates nitroglycerin-induced migraine in trigeminal nucleus caudalis in rats through inhibition of NF-кB[J]. J Headache Pain, 2016, 17(1): 49. http://core.ac.uk/download/pdf/81272789.pdf
    [16]
    张芹, 齐丹丹, 张忠玲. 核转录因子-κB信号通路在偏头痛发病机制中的相关研究进展[J]. 国际神经病学神经外科学杂志, 2016, 43(1): 87-90. https://www.cnki.com.cn/Article/CJFDTOTAL-GWSK201601025.htm
    [17]
    LIOU C J, LEN W B, WU S J, et al. Casticin inhibits COX-2 and iNOS expression via suppression of NF-κB and MAPK signaling in lipopolysaccharide-stimulated mouse macrophages[J]. J Ethnopharmacol, 2014, 158: 310-316. http://www.sciencedirect.com/science/article/pii/S0378874114007569
    [18]
    GALEOTTI N, GHELARDINI C. St. John's wort reversal of meningeal nociception: A natural therapeutic perspective for migraine pain[J]. Phytomedicine, 2013, 20(10): 930-938. http://www.onacademic.com/detail/journal_1000035898239410_d4a7.html
    [19]
    NAGY-GRÖCZ G, TAR L, BOH R Z, et al. The modulatory effect of anandamide on nitroglycerin-induced sensitization in the trigeminal system of the rat[J]. Cephalalgia, 2016, 36(9): 849-861. http://core.ac.uk/download/pdf/80767086.pdf
    [20]
    李国福, 贾佳, 符加红, 等. 异氟烷预处理或后处理对大鼠局灶性脑缺血/再灌注损伤的影响[J]. 中华危重病急救医学, 2014, 26(6): 431-435. https://www.cnki.net/KCMS/detail/detail.aspx?dbcode=IPFD&filename=WZFS201205001213&dbname=IPFD9914
    [21]
    WANG Z, LIU Z, ZHOU L, et al. Immunomodulatory effect of APS and PSP is mediated by Ca2+-cAMP and TLR4/NF-κB signaling pathway in macrophage[J]. Int J Biol Macromol, 2017, 94(Pt A): 283-289.
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(4)  / Tables(1)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (222) PDF downloads(7) Cited by()
    Proportional views
    Related

    Address：No. 287, Changhuai Road, Bengbu, Anhui Province, 233004, P.R.ChinaphoneNo：0552-3066635； 0552-3051890Email：zhqkyx@163.com

    Copyright © Chinese Journal of General Practice皖ICP备2020018345号-2

    Supported by: Beijing Renhe Information Technology Co., Ltd.    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return