miR-200C-3p inhibit the proliferation and migration of prostate cancer cells via targeting ZEB2

LI Hu; ZHU Yong-shi; WANG Ning-ning; GUO Shao-yong; CHEN Zhi-jun

doi:10.16766/j.cnki.issn.1674-4152.002182

Volume 19 Issue 11

Nov. 2021

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Article Navigation > Chinese Journal of General Practice > 2021 > 19(11): 1846-1850

LI Hu, ZHU Yong-shi, WANG Ning-ning, GUO Shao-yong, CHEN Zhi-jun. miR-200C-3p inhibit the proliferation and migration of prostate cancer cells via targeting ZEB2[J]. Chinese Journal of General Practice, 2021, 19(11): 1846-1850. doi: 10.16766/j.cnki.issn.1674-4152.002182

Citation:

LI Hu, ZHU Yong-shi, WANG Ning-ning, GUO Shao-yong, CHEN Zhi-jun. miR-200C-3p inhibit the proliferation and migration of prostate cancer cells via targeting ZEB2[J]. Chinese Journal of General Practice, 2021, 19(11): 1846-1850. doi: 10.16766/j.cnki.issn.1674-4152.002182

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miR-200C-3p inhibit the proliferation and migration of prostate cancer cells via targeting ZEB2

doi: 10.16766/j.cnki.issn.1674-4152.002182

1.
Department of Urology, Anhui Wanbei Coal and Electricity Group General Hospital, Suzhou, Anhui 234000, China

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KJ2019A0355

Received Date: 2021-02-11
Available Online: 2022-02-15

Abstract

Abstract

Objective To explore the effect of miR-200C-3p on prostate cancer cell lines and analyze the potential mechanisms of miR-200C-3p and Zinc finger E-box-binding homeobox 2 (ZEB2). Methods The expression of miR-200C-3p in prostate cancer cell lines PC-3, DU145, and prostate epithelial cells RWPE-1 was detected by qRT-PCR. After DU145 was transfected with miR-200C-3p, the proliferation and migration ability were determined by CCK-8, scratch repair test and Transwell test. After silencing ZEB2, the proliferation and migration ability of DU145 was analyzed, and the potential relationship between miR-200C-3p and ZEB2 was determined by double-luciferase reporter and western blotting. Results The results of qRT-PCR showed that the expression of miR-200C-3p in RWPE-1 was about 2.5 times that of DU145. After DU145 was transfected with miR-200C-3p mimics, the proliferation ability of experimental group was significantly weaker than that of the control group. The healing ability of the experimental group (20.33±1.45) % was significantly lower than that of the control group (46.67±2.40) % (P < 0.001), and the migration ability of the experimental group (114.30±5.21) was significantly lower than that of the control group (212.00±6.49, P < 0.001). Silencing ZEB2 inhibited the proliferation and migration ability of DU145 cells (P < 0.05). The dual luciferase report and western blot experiments confirmed that the overexpression of miR-200C-3p in DU145 reduced the protein expression of ZEB2 (P < 0.05). Conclusion miR-200C-3p inhibits the proliferation and migration of DU145 by mediating ZEB2.
- Prostate cancer,
- miR-200C-3p,
- Zinc finger E-box-binding homeobox 2,
- Proliferation,
- Migration

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miR-200C-3p inhibit the proliferation and migration of prostate cancer cells via targeting ZEB2

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miR-200C-3p inhibit the proliferation and migration of prostate cancer cells via targeting ZEB2

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