Volume 19 Issue 11
Nov.  2021
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Article Navigation >  Chinese Journal of General Practice  > 2021  >  19(11): 1868-1871,1978
LIN Yong-qiang, CHEN Tian-tian, WANG Chao-chao, ZHOU Ying, CAI Xiao-qiao, JIANG Yi, LIN Sheng-fen. The relationship between serum HIF-1α, TGF-β1 and renal interstitial fibrosis and disease progression in patients with chronic kidney disease[J]. Chinese Journal of General Practice, 2021, 19(11): 1868-1871,1978. doi: 10.16766/j.cnki.issn.1674-4152.002188
Citation: LIN Yong-qiang, CHEN Tian-tian, WANG Chao-chao, ZHOU Ying, CAI Xiao-qiao, JIANG Yi, LIN Sheng-fen. The relationship between serum HIF-1α, TGF-β1 and renal interstitial fibrosis and disease progression in patients with chronic kidney disease[J]. Chinese Journal of General Practice, 2021, 19(11): 1868-1871,1978. doi: 10.16766/j.cnki.issn.1674-4152.002188
shu

The relationship between serum HIF-1α, TGF-β1 and renal interstitial fibrosis and disease progression in patients with chronic kidney disease

doi: 10.16766/j.cnki.issn.1674-4152.002188
  • 1.

    Nephrology Department, Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine, Wenzhou, Zhejiang 325000, China

Funds:

 LQ19H050002

  • Received Date: 2021-03-28
    Available Online: 2022-02-15
    Abstract
  •   Objective  To investigate the changes of serum hypoxia-inducible factor-1α (HIF-1α), transforming growth factor (TGF-β1) and tubular interstitial fibrosis (TIF) in patients with chronic kidney disease (CKD) and their predictive value for disease progression.  Methods  A total of 158 cases of CKD patients were selected admitted to Department of Nephrology, Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine from January 2019 to December 2020. The patients were staged according to the glomerular filtration rate (eGFR) value: stage 1 (n=38), stage 2 (n=42), stage 3 (n=30), stage 4 (n=28), stage 5 (n=20). Another 40 cases of healthy subjects were selected as control group. The clinical data and blood samples of above groups were collected. The levels of HIF-1α and TGF-β1 were detected with Enzyme-linked immunosorbent assay (ELISA). TIF area was assessed with Masson staining.  Results  The levels of HIF-1α [(25.48±4.26) ng/mL, (30.98±5.36) ng/mL, (35.88±6.78) ng/mL, (42.25±5.63) ng/mL, (68.96±6.98) ng/mL], TGF-β1 [(3.98±0.36) ng/m, (5.23±0.45) ng/m, (6.78±0.63) ng/m, (8.96±1.52) ng/m, (12.97±2.98) ng/mL] of CKD stage 1-5 were significantly higher than control group [(12.78±0.69) ng/mL and (1.22±0.15) ng/mL, all P < 0.05]. The levels of serum CysC, Scr, HIF-1α, and TGF-β1 of non-TIF group were lower than TIF group (all P < 0.05). The serum HIF-1α, TGF-β1 were positively correlated with serum CysC, Scr, TIF area by Pearson correlate factor analysis (all P < 0.05). The HIF-1α and TGF-β1 can improve the value of early CKD diagnosis by receiver specific curve (ROC).  Conclusion  The increase of serum HIF-1α and TGF-β1 levels is related to the increase of CysC, Scr and TIF. The serum HIF-1α and TGF-β1 can be used as early CKD serum standards, and the combined detection of the two can improve the diagnostic accuracy.

     

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