Regulation of mitochondrial dynamic-related protein 1 on glucose metabolism in liver cancer cells

GENG Xi-lin; ZHANG Ying; LI Hao; ZHANG Yu; CHANG Hu-lin

doi:10.16766/j.cnki.issn.1674-4152.002270

Volume 20 Issue 1

Jan. 2022

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Article Navigation > Chinese Journal of General Practice > 2022 > 20(1): 35-38

GENG Xi-lin, ZHANG Ying, LI Hao, ZHANG Yu, CHANG Hu-lin. Regulation of mitochondrial dynamic-related protein 1 on glucose metabolism in liver cancer cells[J]. Chinese Journal of General Practice, 2022, 20(1): 35-38. doi: 10.16766/j.cnki.issn.1674-4152.002270

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GENG Xi-lin, ZHANG Ying, LI Hao, ZHANG Yu, CHANG Hu-lin. Regulation of mitochondrial dynamic-related protein 1 on glucose metabolism in liver cancer cells[J]. Chinese Journal of General Practice, 2022, 20(1): 35-38. doi: 10.16766/j.cnki.issn.1674-4152.002270

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Regulation of mitochondrial dynamic-related protein 1 on glucose metabolism in liver cancer cells

doi: 10.16766/j.cnki.issn.1674-4152.002270

GENG Xi-lin¹
, ZHANG Ying¹
, LI Hao²
, ZHANG Yu¹
, CHANG Hu-lin^{1
,
,}

1.
Department of Hepatobiliary Surgery, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710032, China

Funds:

2020JQ-948

Received Date: 2020-05-18
Available Online: 2022-03-03

Abstract

Abstract

Objective To explore the effect of mitochondrial dynamic-related protein 1 (DRP1) on glucose metabolism reprogramming in liver cancer cells. Methods After DRP1 was knocked down by siRNA in liver cancer SNU-739 cells, (1) To explore the effect of DRP1 on glycolysis of liver cancer, glucose uptake and lactate production were determined. (2) To explore the effect of DRP1 on oxidative phosphorylation of liver cancer, the oxygen consumption rate and ATP production were determined. (3) The effects of DRP1 knockdown on the levels of metabolites in glycolysis and the TCA cycle were determined by mass spectrometry analysis. Results (1) Down regulation of DRP1significantly inhibited glucose uptake and lactate production in SNU-739 cells (siCtrl: si-DRP1#1: si-DRP1#2=1.000±0.069:0, 417±0.032: 0.400±0.040: F=141.400, P < 0.001) and lactate production (siCtrl: si-DRP1#1: si-DRP1#2= 1.00±0.050:0.327±0.040: 0.310±0.036; F=256.700, P < 0.001). (2) Down regulation of DRP1 markedly increased the rate of oxygen consumption (siCtrl: si-DRP1#1: si-DRP1#2=1.000±0.069: 1.623±0.081: 1.591±0.046; F=81.720, P < 0.001) and ATP production (siCtrl: si-DRP1#1: si-DRP1#2= 1.000±0.062: 1.813±0.093:1.850±0.070; F=119.200, P < 0.001). (3) Down regulation of DRP1 decreased the levels of glucose, pyruvate and lactate (all P < 0.001) in glycolysis metabolism, but increased the levels of citrate, fumarate and malate (all P < 0.001) metabolites in the TCA cycle. Conclusion DRP1 reprogrammed glucose metabolism of liver cancer cells through increasing glycolysis and decreasing oxidative phosphorylation.
- Mitochondrial dynamic-related protein 1,
- Mitochondria,
- Glycolysis,
- Oxidative phosphorylation,
- Liver cancer

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References

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Regulation of mitochondrial dynamic-related protein 1 on glucose metabolism in liver cancer cells

doi: 10.16766/j.cnki.issn.1674-4152.002270