Volume 20 Issue 3
Mar.  2022
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Article Navigation >  Chinese Journal of General Practice  > 2022  >  20(3): 403-406
CAI Bao-xiang, ZHU Jian-wei, CHEN Yan, JIN Ying-kai. Detection and significance of plasma exosomes miR-21 and lncRNA MALAT1 in patients with atopic dermatitis[J]. Chinese Journal of General Practice, 2022, 20(3): 403-406. doi: 10.16766/j.cnki.issn.1674-4152.002363
Citation: CAI Bao-xiang, ZHU Jian-wei, CHEN Yan, JIN Ying-kai. Detection and significance of plasma exosomes miR-21 and lncRNA MALAT1 in patients with atopic dermatitis[J]. Chinese Journal of General Practice, 2022, 20(3): 403-406. doi: 10.16766/j.cnki.issn.1674-4152.002363
shu

Detection and significance of plasma exosomes miR-21 and lncRNA MALAT1 in patients with atopic dermatitis

doi: 10.16766/j.cnki.issn.1674-4152.002363
  • 1.

    Department of Dermatology, Ningbo Medical Center Li Huili Hospital, Ningbo, Zhejiang 315040, China

Funds:

 LY20H070006

  • Received Date: 2021-05-20
    Available Online: 2022-08-13
    Abstract
  •   Objective  To analyse the expression levels of plasma exosome microRNA-21 (miR-21) and long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in patients with atopic dermatitis (AD) and to explore their clinical significance.  Methods  A total of 118 patients with AD in the Dermatology Department of Ningbo Medical Center Li Huili Hospital from January 2018 to May 2020 were selected and divided into 41 cases of mild, 38 cases of moderate and 39 cases of severe according to the scoring atopic dermatitis index (SCORAD). At the same time, 120 healthy people were selected as the control group. Plasma exosomes were extracted and identified. The levels of miR-21 and lncRNA MALAT1 in plasma exosomes were measured by real-time fluorescence quantitative polymerase chain reaction. The Pearson method was used to analyse the correlation among miR-21, lncRNA MALAT1 and SCORAD score, and multiple linear stepwise regression model was used to analyse the risk factors of increased SCORAD score in patients with AD.  Results  The extracted samples had the characteristics of exosomes. The levels of serum miR-21, lncRNA MALAT1 and SCORAD score in the mild, moderate and severe patients in the AD group were significantly higher than those in the control group and increased with the increase in disease severity (F=91.160, 206.502, 246.615, all P < 0.001). The levels of miR-21 and lncRNA MALAT1 in plasma exosomes were positively correlated with SCORAD score (r=0.631, 0.528, all P < 0.05). The high levels of plasma exosomes miR-21 and lncRNA MALAT1 were the risk factors for the increase in SCORAD score in patients with AD (all P < 0.05).  Conclusion  The abnormally high expression levels of plasma exosomes miR-21 and lncRNA MALAT1 may be related to the occurrence and development of AD and closely related to disease severity. Monitoring the levels of miR-21 and lncRNA MALAT1 may be important for further targeted prevention and treatment of AD.

     

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