Volume 20 Issue 4
Apr.  2022
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Article Navigation >  Chinese Journal of General Practice  > 2022  >  20(4): 550-554
ZHU Qing-qing, SUN Chen, SUN Jian-li, GAO Zhen-jie. Effect and mechanism of low ENPP1 expression on the epithelial-mesenchymal transition of oral squamous cell carcinoma cell line[J]. Chinese Journal of General Practice, 2022, 20(4): 550-554. doi: 10.16766/j.cnki.issn.1674-4152.002398
Citation: ZHU Qing-qing, SUN Chen, SUN Jian-li, GAO Zhen-jie. Effect and mechanism of low ENPP1 expression on the epithelial-mesenchymal transition of oral squamous cell carcinoma cell line[J]. Chinese Journal of General Practice, 2022, 20(4): 550-554. doi: 10.16766/j.cnki.issn.1674-4152.002398
shu

Effect and mechanism of low ENPP1 expression on the epithelial-mesenchymal transition of oral squamous cell carcinoma cell line

doi: 10.16766/j.cnki.issn.1674-4152.002398
  • 1.

    Department of Dental Implantology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, China

Funds:

 LHGJ20190189

  • Received Date: 2021-04-18
    Available Online: 2022-08-20
    Abstract
  •   Objective  To investigate the effect of low expression ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) on the epithelial-mesenchymal transition (EMT) in oral squamous cell carcinoma.  Methods  The normal human oral mucosal epithelial cell line and human oral squamous cell carcinoma CAL-27 cells were cultured and subcultured, the expression of ENPP1 mRNA was detected. CAL-27 cells in logarithmic growth stage were transfected and divided into blank group (without treatment), control group (transfected with NC-siRNA plasmid) and transfection group (transfected with ENPP1-siRNA). The expression of ENPP1 mRNA and protein were detected. CAL-27 cells in logarithmic growth stage were transfected and divided into group A (not transfected), group B (transfected with NC-siRNA plasmid) and group C (transfected with ENPP1-siRNA plasmid + MAPK agonist), group D (transfected with ENPP1-siRNA plasmid), group E (MAPK agonist). The proliferation activity, number of invasive cells, migration ability and protein expression of phosphorylated-mitogen activated protein kinase (p-MAPK) and phosphorylated-extracellular regulated kinase 1/2 (p-ERK1/2) were detected.  Results  Absorbance (A) of each period, group D < group C < group A, group B < group E (all P < 0.05). The number of invasive cells in five groups were (85.23±2.22) cells/field, (85.41±1.59) cells/field, (53.67±2.10) cells/field, (37.39±2.06) cells/field and (111.08±5.20) cells/field cells/field, respectively, group D < group C < group A, group B < group E (all P < 0.05). The percentage of scratch width: group E < group A, group B < group C < group D (all P < 0.05). The relative expression levels of p-MAPK, p-ERK1 and p-ERK2 proteins: group D < group C < group A, group B < group E (all P < 0.05).  Conclusion  Low expression of ENPP1 can inhibit EMT, the mechanism may be related to the inhibition of MAPK signaling pathway.

     

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    • References(21)
  • [1]
    刘璐璐, 李秀川, 袁冯, 等. 加速康复外科理念在游离皮瓣修复口腔癌患者中的应用[J]. 中华全科医学, 2020, 18(10): 1774-1778. doi: 10.16766/j.cnki.issn.1674-4152.001616

    LIU L L, LI X C, YUAN F, et al. Application of the concept of enhanced recovery after surgery in free flap repair of oral cancer patients[J]. Chinese Journal of General Practice, 2020, 18(10): 1774-1778. doi: 10.16766/j.cnki.issn.1674-4152.001616
    [2]
    SHETTY S S, SHARMA M, FONSECA F P, et al. Signaling pathways promoting epithelial mesenchymal transition in oral submucous fibrosis and oral squamous cell carcinoma[J]. Jpn Dent Sci Rev, 2020, 56(1): 97-108. doi: 10.1016/j.jdsr.2020.07.002
    [3]
    LUO Y D, DING X, DU H M, et al. FOXM1 is a novel predictor of recurrence in patients with oral squamous cell carcinoma associated with an increase in epithelial-mesenchymal transition[J]. Mol Med Rep Vol, 2019, 19(5): 4101-4108.
    [4]
    赵伟萍, 李合芳, 马妍敏, 等. 苦参碱注射液对中晚期口腔癌患者炎症、凋亡及侵袭转移相关因子表达的影响[J]. 河北医药, 2020, 42(3): 378-381. doi: 10.3969/j.issn.1002-7386.2020.03.013

    ZHAO W P, LI H F, MA Y M, et al. Effects of matrine injection on expressions of inflammation, apoptosis and invasion and metastasis-related factors in patients with advanced oral cancer[J]. Hebei Medical Journal, 2020, 42(3): 378-381. doi: 10.3969/j.issn.1002-7386.2020.03.013
    [5]
    HU M, GUO W, LIAO Y, et al. Dysregulated ENPP1 increases the malignancy of human lung cancer by inducing epithelial-mesenchymal transition phenotypes and stem cell features[J]. Am J Cancer Res, 2019, 9(1): 134-144.
    [6]
    KAWECKI, NEDEVA I R, ILOYA J, et al. Mouth cancer awareness in general population: Results from grampian region of scotland, united kingdom[J]. J Oral Maxillofac Res, 2019, 10(2): e3-e5.
    [7]
    GHAFOURI F S, GHOLIPOUR M, TAHERI M, et al. MicroRNA profile in the squamous cell carcinoma: Prognostic and diagnostic roles[J]. Heli, 2020, 6(11): e05436. DOI: 10.1016/j.heliyon.2020.e05436.
    [8]
    LEE C H. Reversal of epithelial-mesenchymal transition by natural anti-inflammatory and pro-resolving lipids[J]. 1841. DOI: 10.3390/cancers11121841.
    [9]
    WILLIAMS E D, GAO D, REDFERN A, et al. Controversies around epithelial-mesenchymal plasticity in cancer metastasiss[J]. Nat Rev Cancer, 2019, 19(12): 716-732. doi: 10.1038/s41568-019-0213-x
    [10]
    CURTIS V F, CARTWRIGHT I M, LEE J S, et al. Neutrophils as sources of dinucleotide polyphosphates and metabolism by epithelial ENPP1 to influence barrier function via adenosine signaling[J]. Mol Biol Cell, 2018, 29(22): 2687-2699. doi: 10.1091/mbc.E18-06-0377
    [11]
    STABACH P R, ZIMMERMAN K, ADAME A, et al. Improving the pharmacodynamics and in vivo activity of ENPP1-Fc through protein and glycosylation engineering[J]. Clin Transl Sci, 2021, 14(1): 362-372. doi: 10.1111/cts.12887
    [12]
    OHEIM R, ZIMMERMAN K, MAULDING N D, et al. Human heterozygous ENPP1 deficiency is associated with early onset osteoporosis, a phenotype recapitulated in a mouse model of Enpp1 deficiency[J]. J Bone Miner Res, 2020, 35(3): 528-539. doi: 10.1002/jbmr.3911
    [13]
    WANG H, YE F, ZHOU C, et al. High expression of ENPP1 in high-grade serous ovarian carcinoma predicts poor prognosis and as a molecular therapy target[J]. PLoS One, 2021, 16(2): e0245733. DOI: 10.1371/journal.pone.0245733.
    [14]
    ONYEDIBE K I, WANG M, SINTIM H O. ENPP1, an old enzyme with New functions, and small molecule inhibitors-A STING in the tale of ENPP1[J]. Mole, 2019, 24(22): 4192-4193. doi: 10.3390/molecules24224192
    [15]
    KOTWAL A, FERRER A, KUMAR R, et al. Clinical and biochemical phenotypes in a family with ENPP1 mutations[J]. J Bone Miner Res, 2020, 35(4): 662-670. doi: 10.1002/jbmr.3938
    [16]
    马超, 张韬, 林润台, 等. ENPP1诱导肿瘤干性和上皮间质转化促进口腔鳞癌的发展[J]. 现代口腔医学杂志, 2020, 34(3): 129-132. https://www.cnki.com.cn/Article/CJFDTOTAL-XDKY202003003.htm

    MA C, ZHANG T, LIN R T, et al. ENPP1 induces stemness and epithelial-mesenchymal transition in oral squamous cell carcinoma[J]. Journal of contemporary stomatology, 2020, 34(3): 129-132. https://www.cnki.com.cn/Article/CJFDTOTAL-XDKY202003003.htm
    [17]
    BRAICU C, BUSE M, BUSUIOC C, et al. A comprehensive review on MAPK: A promising therapeutic target in cancer[J]. Cancers, 2019, 11(10): 1618-1619. doi: 10.3390/cancers11101618
    [18]
    DUSHANE J K, MAGINNIS M S. Human DNA virus exploitation of the MAPK-ERK cascade[J]. Int J Mol Sci, 2019, 20(14): 3427-3428. doi: 10.3390/ijms20143427
    [19]
    林镇海, 闫士灿, 张洁筠, 等. MST4通过激活MAPK-ERK信号通路调节炎症因子释放促进肝癌的侵袭和转移的机制研究[J]. 中国癌症杂志, 2017, 27(9): 681-686. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGAZ201709001.htm

    LIN Z H, YAN S C, ZHANG J Y, et al. MST4 upregulates secretion of inflammatory factors via activation of MAPK-ERK signaling pathway and promotes invasion and metastasis of hepatocellular carcinoma[J]. China Oncology, 2017, 27(9): 681-686. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGAZ201709001.htm
    [20]
    朱晨笛, 郭慕真, 蔡倩, 等. MAPK信号通路在百草枯诱导上皮-间充质改变中的作用[J]. 中华劳动卫生职业病杂志, 2018, 36(8): 561-567. doi: 10.3760/cma.j.issn.1001-9391.2018.08.001

    ZHU C D, GUO M Z, CAI Q, et al. Role of MAPK signaling pathway in epithelial-mesenchymal transition of type Ⅱ alveolar epithelial cells induced by Paraquat[J]. Chinese Journal of Industrial Hygiene and Occupational Diseases, 2018, 36(8): 561-567. doi: 10.3760/cma.j.issn.1001-9391.2018.08.001
    [21]
    CHEN K, LIU M X, MAK C S, et al. Methylation-associated silencing of miR-193a-3p promotes ovarian cancer aggressiveness by targeting GRB7 and MAPK/ERK pathways[J]. Theranostics, 2018, 8(2): 423-436. doi: 10.7150/thno.22377
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