Effect of Panax notoginseng saponins on MPO activity and radiotherapy sensitivity of cervical cancer based on the HSP70 signalling pathway

ZHOU Tao-qi; CHEN Jun; ZHOU Xin; PANG Lin-rong; YANG Wei-li; XU Yong

doi:10.16766/j.cnki.issn.1674-4152.002401

Volume 20 Issue 4

Apr. 2022

Turn off MathJax

Article Contents

Article Navigation > Chinese Journal of General Practice > 2022 > 20(4): 565-569

ZHOU Tao-qi, CHEN Jun, ZHOU Xin, PANG Lin-rong, YANG Wei-li, XU Yong. Effect of Panax notoginseng saponins on MPO activity and radiotherapy sensitivity of cervical cancer based on the HSP70 signalling pathway[J]. Chinese Journal of General Practice, 2022, 20(4): 565-569. doi: 10.16766/j.cnki.issn.1674-4152.002401

Citation:

ZHOU Tao-qi, CHEN Jun, ZHOU Xin, PANG Lin-rong, YANG Wei-li, XU Yong. Effect of Panax notoginseng saponins on MPO activity and radiotherapy sensitivity of cervical cancer based on the HSP70 signalling pathway[J]. Chinese Journal of General Practice, 2022, 20(4): 565-569. doi: 10.16766/j.cnki.issn.1674-4152.002401

Citation:

ZHOU Tao-qi, CHEN Jun, ZHOU Xin, PANG Lin-rong, YANG Wei-li, XU Yong. Effect of Panax notoginseng saponins on MPO activity and radiotherapy sensitivity of cervical cancer based on the HSP70 signalling pathway[J]. Chinese Journal of General Practice, 2022, 20(4): 565-569. doi: 10.16766/j.cnki.issn.1674-4152.002401

PDF( 1386 KB)

Effect of Panax notoginseng saponins on MPO activity and radiotherapy sensitivity of cervical cancer based on the HSP70 signalling pathway

doi: 10.16766/j.cnki.issn.1674-4152.002401

1.
Radiotherapy and Chemotherapy Center, the Affiliated People ' s Hospital of Ningbo University, Ningbo, Zhejiang 315000, China

Funds:

2019RC274

Received Date: 2021-11-08
Available Online: 2022-08-20

Abstract

Abstract

Objective To explore the effect of Panax notoginseng saponins (PNS) on myeloperoxidase (MPO) activity and radiotherapy sensitivity of cervical cancer based on the heat shock protein 70 (HSP70) signalling pathway. Methods The exponential growth against cervical cancer cell lines were randomly divided into cervical cancer radiotherapy (group A, n=6), low doses of PNS group (group B, n=6), high dose PNS group (group C, n=6), gold finches flavin group (group D, n=6). Another group of HeLa cells was taken and HSP70 inhibitor was added, which was recorded as group E (n=6). The expression of HSP70 protein was detected by immunohistochemistry, and MPO activity was detected by using a spectrophotometer. After radiotherapy, the activity of cervical cancer cells was detected by DAPI staining, MTT assay and chamber assay. Results The expression levels of HSP70 protein in groups A, B, C and D were 3.51±0.13, 2.33±0.12, 1.25±0.11 and 1.26±0.12, respectively, and the differences were statistically significant (F=21.090, P < 0.001). The expression of HSP70 protein, MPO activity, proliferation rate and invasion number of cells in groups B, C and D were significantly decreased (all P < 0.05), and those in group C were significantly lower than those in group B (all P < 0.05). However, there was no statistically significant difference between group D and group C (all P>0.05). Compared with group A, the apoptosis rate of groups B, C and D was significantly increased (all P < 0.05), and the apoptosis rate of group C was significantly higher than that of group B (all P < 0.05). Moreover, there was no statistically significant difference between group D and group C (P>0.05). Compared with group A, Bax protein expression in groups B, C, D and E was significantly increased (all P < 0.05), and Bcl-2 protein expression was significantly decreased (all P < 0.05). The expression of these proteins in group C was significantly changed compared with that in group B (all P < 0.05). No statistically significant difference was observed between groups E, D and C (all P>0.05). Conclusion Panax notoginseng saponins can significantly reduce MPO activity and improve radiotherapy sensitivity of cervical cancer, and the mechanism may be related to the negative regulation of the HSP70 signalling pathway.
- HSP70 signalling pathway,
- Panax notoginseng saponins,
- Cervical cancer,
- Myeloperoxidase activity,
- Radiotherapy sensitivity

FullText(HTML)

References(22)

References

[1]	汪瑶, 王春兰, 俞惠艳, 等. 宫颈癌术后带导尿管出院患者自我管理能力的影响因素研究[J]. 中华全科医学, 2021, 19(10): 1772-1774. doi: 10.16766/j.cnki.issn.1674-4152.002164 WANG Y, WANG C L, YU H Y, et al. Studies on the influencing factors of self-management of discharged patients with urinary catheters after cervical cancer surgery[J]. Chinese Journal of General Practice, 2021, 19(10): 1772-1774. doi: 10.16766/j.cnki.issn.1674-4152.002164
[2]	马欢, 张贤雨, 张飞, 等. miR-193b靶向调节cyclin D1对宫颈癌细胞放疗敏感性的影响[J]. 中华内分泌外科杂志, 2020, 14(6): 482-486. doi: 10.3760/cma.j.cn.115807-20201007-00306 MA H, ZHANG X Y, ZHANG F, et al. Effect of miR-193b on radiosensitivity of cervical cancer cells by targeting cyclin D1[J]. Chinese Journal of Endocrine Surgery, 2020, 14(6): 482-486. doi: 10.3760/cma.j.cn.115807-20201007-00306
[3]	王军, 袁纯辉, 孙红, 等. 高危型人乳头瘤病毒16 E6蛋白通过外泌体传递热休克蛋白70调控巨噬细胞介导宫颈癌细胞增殖, 侵袭和迁移[J]. 中华微生物学和免疫学杂志, 2020, 40(7): 538-546. doi: 10.3760/cma.j.cn112309-20190822-00267 WANG J, YUAN C H, SUN H, et al. High-risk human papillomavirus 16 E6 protein regulates tumor-associated macrophages through exosomal heat shock protein 70 to promote cervical cancer cell C33A proliferation, invasion and migration[J]. Chinese Journal of Microbiology and Immunology, 2020, 40(7): 538-546. doi: 10.3760/cma.j.cn112309-20190822-00267
[4]	ZEINDLER J, ANGEHRN F, DROESER R, et al. Infiltration by myeloperoxidase-positive neutrophils is an independent prognostic factor in breast cancer[J]. Breast Cancer Res Treat, 2019, 177(3): 581-589. doi: 10.1007/s10549-019-05336-3
[5]	魏剑锋, 许泽波, 王伟, 等. 三七总皂苷对肝癌细胞株HepG2的钙离子平衡和生长的影响[J]. 中华实验外科杂志, 2020, 37(10): 1815-1818. doi: 10.3760/cma.j.cn421213-20200122-01033 WEI J F, XU Z B, WANG W, et al. Effects of PANAX Notoginseng saponins on calcium balance and growth of hepatocellular carcinoma cell line HepG2[J]. Chinese Journal of Experimental Surgery, 2020, 37(10): 1815-1818. doi: 10.3760/cma.j.cn421213-20200122-01033
[6]	吕红青, 张琳, 倪镌. 帕唑帕尼联合放疗对宫颈癌的治疗效果及对血清VEGFR水平的影响[J]. 中华全科医学, 2020, 18(6): 934-937. doi: 10.16766/j.cnki.issn.1674-4152.001396 LYU H Q, ZHANG L, NI J. Therapeutic effect of pazopanib combined with radiotherapy on cervical cancer and its effect on serum levels of vascular endothelial growth factor receptor[J]. Chinese Journal of General Practice, 2020, 18(6): 934-937. doi: 10.16766/j.cnki.issn.1674-4152.001396
[7]	姚黎超, 王伟, 武伦, 等. 三七总皂苷对人胰腺癌PANC-1细胞增殖, 凋亡的影响[J]. 肿瘤药学, 2019, 9(6): 870-874. doi: 10.3969/j.issn.2095-1264.2019.06.008 YAO L C, WANG W, WU L, et al. Effects of PANAX Notoginseng saponins on proliferation and apoptosis of human pancreatic cancer PANC-1 cells[J]. Anti-tumor Pharmacy, 2019, 9(6): 870-874. doi: 10.3969/j.issn.2095-1264.2019.06.008
[8]	张芳, 王雅莉, 李萌萌, 等. 金雀异黄素对白介素-6诱导下SKOV3人卵巢癌细胞的抑制作用[J]. 毒理学杂志, 2019, 33(1): 12-17. https://www.cnki.com.cn/Article/CJFDTOTAL-WSDL201901002.htm ZHANG F, WANG Y L, LI M M, et al. Inhibition of genistein on interleukin-6-induced human ovarian cancer SKOV3 cells[J]. Journal of Toxicology, 2019, 33(1): 12-17. https://www.cnki.com.cn/Article/CJFDTOTAL-WSDL201901002.htm
[9]	CHEN L, CHEN M Y, SHAO L, et al. PANAX Notoginseng saponins prevent colitis-associated colorectal cancer development: The role of gut microbiota[J]. Chin J Nat Med, 2020, 18(7): 500-507.
[10]	CHENG Y, BAO D, CHEN X, et al. Microwave-triggered/HSP-targeted gold nano-system for triple-negative breast cancer photothermal therapy[J]. Int J Pharm, 2021, 593: 120162. DOI: 10.1016/j.ijpharm.2020.120162.
[11]	段智, 李代强, 陈辉, 等. 宫颈癌患者人乳头瘤病毒感染和HSP70表达及Treg细胞分布研究[J]. 中华医院感染学杂志, 2020, 30(5): 729-733. https://www.cnki.com.cn/Article/CJFDTOTAL-ZHYY202005022.htm DUAN Z, LI D Q, CHEN H, et al. Heat shock protein 70 and regulatory T cells in cervical cancer patients with human papillomavirus infection[J]. Chinese Journal of Nosocomiology, 2020, 30(5): 729-733. https://www.cnki.com.cn/Article/CJFDTOTAL-ZHYY202005022.htm
[12]	苏玉强, 范晓英, 秦艳, 等. 三七总皂苷调节丙泊酚所致大鼠认知功能损伤的实验研究[J]. 海南医学院学报, 2019, 25(10): 739-743. https://www.cnki.com.cn/Article/CJFDTOTAL-HNYY201910006.htm SU Y Q, FAN X Y, QIN Y, et al. Effects of PANAX Notoginseng saponins on apoptosis, inflammation and oxidative stress in rats with propofol-induced cognitive dysfunction[J]. Journal of Hainan Medical University, 2019, 25(10): 739-743. https://www.cnki.com.cn/Article/CJFDTOTAL-HNYY201910006.htm
[13]	柳俊, 陈军, 鲍晨, 等. HSP70、eIF4E、DNMT1在宫颈癌中的表达及意义[J]. 现代生物医学进展, 2020, 20(20): 3988-3991. https://www.cnki.com.cn/Article/CJFDTOTAL-SWCX202020041.htm LIU J, CHEN J, BAO C, et al. Expression and significance of HSP70, eIF4E and DNMT1 in cervical cancer[J]. Progress in Modern Biomedicine, 2020, 20(20): 3988-3991. https://www.cnki.com.cn/Article/CJFDTOTAL-SWCX202020041.htm
[14]	YANG W J, WANG M Y, PAN F Z, et al. Association between MPO-463G>A polymorphism and cancer risk: Evidence from 60 case-control studies[J]. World J Surg Oncol, 2017, 15(1): 144. doi: 10.1186/s12957-017-1183-7
[15]	梁海清, 李一然, 张淑燕, 等. 髓过氧化物酶与结直肠癌相关性的研究进展[J]. 中国医师杂志, 2021, 23(6): 958-960, 963. doi: 10.3760/cma.j.cn431274-20200523-00655 LIANG H Q, LI Y R, ZHANG S Y, et al. Research and development of correlation between myeloperoxidaseand colorectal cancer[J]. Journal of Chinese Physician, 2021, 23(6): 958-960, 963. doi: 10.3760/cma.j.cn431274-20200523-00655
[16]	SHI X, LI B, YUAN Y, et al. The possible association between the presence of an MPO-463 G>A (rs2333227) polymorphism and cervical cancer risk[J]. Pathol Res Pract, 2018, 214(8): 1142-1148. doi: 10.1016/j.prp.2018.05.018
[17]	ROSZAK A, LUTKOWSKA A, LIANERI M, et al. Involvement of myeloperoxidase gene polymorphism 463G>A in development of cervical squamous cell carcinoma[J]. Int J Biol Markers, 2016, 31(4): e440-e445. doi: 10.5301/jbm.5000212
[18]	王雯智, 郑蕾, 李虹. 宫颈癌中miR-15b, DCLK1的表达变化及与放疗敏感性的关系[J]. 河北医药, 2020, 42(3): 330-334. doi: 10.3969/j.issn.1002-7386.2020.03.002 WANG W Z, ZHENG L, LI H. The correlation between the expression changes of MiR-15b, DCLK1 and radiotherapy sensitivity in patients with cervical cancer[J]. Hebei Medical Journal, 2020, 42(3): 330-334. doi: 10.3969/j.issn.1002-7386.2020.03.002
[19]	万莉, 比丽克孜·艾克木, 刘娜. 辛伐他汀通过调控Chk1基因的表达对宫颈癌细胞增殖, 凋亡以及放疗敏感性的影响[J]. 现代肿瘤医学, 2020, 28(18): 3138-3144. doi: 10.3969/j.issn.1672-4992.2020.18.009 WAN L, BILIZI A, LIU N. Effect of simvastatin on proliferation, apoptosis and radiosensitivity of cervical cancer cells by regulating the expression of Chk1 gene[J]. Journal of Modern Oncology, 2020, 28(18): 3138-3144. doi: 10.3969/j.issn.1672-4992.2020.18.009
[20]	白桦. miR-451靶向MIF调控宫颈癌细胞生物学功能并通过外泌体释放影响其化疗敏感性[D]. 太原: 山西医科大学, 2020. BAI H. The targeting of miR-451 with MIF regulates the biological function of cervical cancer cells and affects its chemosensitivity through exosome release[D]. Taiyuan: Shanxi Medical University, 2020.
[21]	邹存华. 三七总皂苷抑制Hela细胞增殖的实验研究[D]. 青岛: 青岛大学, 2012. ZOU C H. Effect of total saponins of Panax Notoginseng on the proliferation of Hela cells[D]. Qingdao: Qingdao University, 2012.
[22]	YAO L C, WU L, WANG W, et al. Panax notoginseng saponins promote cell death and chemosensitivity in pancreatic cancer through the apoptosis and autophagy pathways[J]. Anticancer Agents Med Chem, 2021, 21(13): 1680-1688. doi: 10.2174/1871520620999201110191459

Relative Articles

Supplements(0)

Cited By

Proportional views

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Figures(4) / Tables(2)

Get Citation

PDF

XML

Article Metrics

Article views (264) PDF downloads(4)

Effect of Panax notoginseng saponins on MPO activity and radiotherapy sensitivity of cervical cancer based on the HSP70 signalling pathway

doi: 10.16766/j.cnki.issn.1674-4152.002401

Abstract

References

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Proportional views

Related

Effect of Panax notoginseng saponins on MPO activity and radiotherapy sensitivity of cervical cancer based on the HSP70 signalling pathway

doi: 10.16766/j.cnki.issn.1674-4152.002401

Abstract

References

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Proportional views

Related

Export File

Citation

Format

Content