Volume 20 Issue 5
May  2022
Turn off MathJax
Article Contents
Article Navigation >  Chinese Journal of General Practice  > 2022  >  20(5): 727-730
SHI Wei-hua, CHAO Xiao-ying, SU Yong-xing, LI Shuo, LI Xiao-li, ZHONG Guo-qiang. Mechanism of Shenfu Decoction in the treatment of atrial fibrillation based on network pharmacology and molecular docking[J]. Chinese Journal of General Practice, 2022, 20(5): 727-730. doi: 10.16766/j.cnki.issn.1674-4152.002440
Citation: SHI Wei-hua, CHAO Xiao-ying, SU Yong-xing, LI Shuo, LI Xiao-li, ZHONG Guo-qiang. Mechanism of Shenfu Decoction in the treatment of atrial fibrillation based on network pharmacology and molecular docking[J]. Chinese Journal of General Practice, 2022, 20(5): 727-730. doi: 10.16766/j.cnki.issn.1674-4152.002440
shu

Mechanism of Shenfu Decoction in the treatment of atrial fibrillation based on network pharmacology and molecular docking

doi: 10.16766/j.cnki.issn.1674-4152.002440

  • Cardiovascular Department, the First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, China

Funds:

 82060068

  • Received Date: 2021-08-19
    Available Online: 2022-09-05
    Abstract
  •   Objective  To explore the mechanism of Shenfu Decoction in the treatment of atrial fibrillation by network pharmacology and molecular docking technology.  Methods  The main active ingredients of Shenfu Decoction were screened by the TCMSP database, and the related targets were annotated by the Uniprot database and gene names were obtained. The targets of atrial fibrillation were obtained from the OMIM and Pharmgkb Databases. R software was used to determine the intersection of atrial fibrillation and the active ingredient targets of Shenfu Decoction, and the therapeutic targets were obtained. The PPI network was constructed by using the online String database, and the results were imported into Cytoscape software to construct the "active ingredient-target-disease" network and perform topology analysis to obtain the core targets. The GO and KEGG enrichment analyses of key targets in the DAVID database were carried out, and AutoDock Vina software was applied to conduct molecular docking of key components and key targets.  Results  The 101 targets of Shenfu Decoction and 3 219 targets of atrial fibrillation were obtained, and 65 common targets were obtained after the intersection. Topology analysis showed that NFKBIA, RELA, IL1B, JUN, STAT1 and MAPK8 might be the core targets of Shenfu Decoction in treating AF. GO suggested that the targets involved biological processes such as voltage-gated calcium channel activity, calcium ion transport and reactive oxygen metabolism. KEGG suggested that the targets were involved in the treatment of atrial fibrillation through cGMP-PKG, cAMP, calcium channel and PI3K-Akt signalling pathways. The results of molecular docking showed that the core target protein had good binding stability with the corresponding active components.  Conclusion  The treatment of atrial fibrillation with Shenfu Decoction has the characteristics of "multi-component, multi-target and multi-pathway", which may be related to targets of NFKBIA, RELA, IL1B, JUN, STAT1 and MAPK8. The specific mechanisms may need to be confirmed by molecular biological experiments.

     

    • FullText(HTML)
  • loading
    • References(21)
  • [1]
    黄从新, 张澍, 黄德嘉, 等. 心房颤动: 目前的认识和治疗的建议(2018)[J]. 中国心脏起搏与心电生理杂志, 2018, 32(4): 315-368. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGXZ201804002.htm

    HUANG C X, ZHANG S, HUANG D J, et al. Atrial fibrillation: current understanding and treatment suggestions (2018)[J]. Chinese Journal of cardiac pacing and electrophysiology, 2018, 32(4): 315-368. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGXZ201804002.htm
    [2]
    张湘卓, 李杰, 曾雪芹, 等. 浅析心悸病证论治[J]. 时珍国医国药, 2020, 31(2): 387-389. https://www.cnki.com.cn/Article/CJFDTOTAL-SZGY202002045.htm

    ZHANG X Z, LI J, ZENG X Q, et al. Analysis of the treatment of palpitation syndrome[J]. Shi Zhen, National Medicine, 2020, 31(2): 387-389. https://www.cnki.com.cn/Article/CJFDTOTAL-SZGY202002045.htm
    [3]
    史默怡, 陈晓喆, 符德玉. 符德玉教授综合疗法治疗心悸经验[J]. 中西医结合心脑血管病杂志, 2021, 19(5): 866-867. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYYY202105043.htm

    SHI M Y, CHEN X Z, FU D Y. Professor Fu Deyu's experience of comprehensive therapy in the treatment of palpitations[J]. Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease, 2021, 19(5): 866-867. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYYY202105043.htm
    [4]
    李平平, 乔会侠, 李耀辉. 稳心颗粒联合胺碘酮治疗阵发性房颤气阴两虚证的临床效果[J]. 临床医学研究与实践, 2020, 5(35): 152-154. https://www.cnki.com.cn/Article/CJFDTOTAL-YLYS202035056.htm

    LI P P. QIAO H X, LI Y H. Clinical effect of Wenxin Granule Combined with amiodarone in the treatment of Qi Yin deficiency syndrome of paroxysmal atrial fibrillation[J]. Clinical medical research and practice, 2020, 5(35): 152-154. https://www.cnki.com.cn/Article/CJFDTOTAL-YLYS202035056.htm
    [5]
    曾雪亮, 李蓓, 曾韬慧, 等. 穿心莲内酯抗心律失常作用及机制研究[J]. 中国医学创新, 2021, 18(15): 7-10. doi: 10.3969/j.issn.1674-4985.2021.15.002

    ZENG X L, LI B, ZENG T H, et al. Study on the antiarrhythmic effect and mechanism of andrographolide[J]. Chinese medical innovation, 2021, 18(15): 7-10. doi: 10.3969/j.issn.1674-4985.2021.15.002
    [6]
    NI J Y, SHI Y, LI L, et al. Cardioprotection against heart failure by Shenfu injection via TGF-β/Smads signaling pathway[J]. Evid Based Complement Alternat Med, 2017, 2017: 7083016. DOI: 10.1155/2017/7083016.
    [7]
    陈伟华, 李吉宗. 参附汤合苓桂术甘汤加减对慢性心力衰竭患者心功能及NT-proBNP的调节[J]. 中医临床研究, 2020, 12(1): 72-74. doi: 10.3969/j.issn.1674-7860.2020.01.025

    CHEN W H, LI J Z, et al. Regulation of Shenfu Decoction and Linggui Zhugan Decoction on cardiac function and NT proBNP in patients with chronic heart failure[J]. Clinical research of traditional Chinese medicine, 2020, 12(1): 72-74. doi: 10.3969/j.issn.1674-7860.2020.01.025
    [8]
    黎彩凤, 张丰荣, 祝娜, 等. 彝族药金胃泰胶囊治疗胃肠疾病的网络药理学研究[J]. 中国中药杂志, 2021, 46(4): 865-876. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY202104017.htm

    LI C F, ZHANG F R, ZHU N, et al. Network pharmacological study of Yi medicine jinweitai capsule in the treatment of gastrointestinal diseases[J]. Chinese Journal of traditional Chinese medicine, 2021, 46(4): 865-876. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY202104017.htm
    [9]
    ZIMETBAUM P. Atrial Fibrillation[J]. Ann Intern Med, 2017, 166(5): Itc33-Itc48. DOI: 10.7326/AITC201703070.
    [10]
    朱静, 徐健, 苏浩, 等. 左心耳封堵治疗非瓣膜性房颤的安全性和有效性[J]. 中华全科医学, 2020, 18(8): 1261-1264. doi: 10.16766/j.cnki.issn.1674-4152.001480

    ZHU J, XU J, SU H, et al. Safety and efficacy of left atrial appendage occlusion in the treatment of nonvalvular atrial fibrillation[J]. Chinese general practice, 2020, 18(8): 1261-1264. doi: 10.16766/j.cnki.issn.1674-4152.001480
    [11]
    LI J H, GAO M, ZHANG M W, et al. Treatment of atrial fibrillation: A comprehensive review and practice guide[J]. Cardiovasc J Afr, 2020, 31(3): 153-158.
    [12]
    FAN X H, YU Y Y, LAN H, et al. Ca2+/calmodulin-dependent protein kinase Ⅱ (CaMKⅡ) increases small-conductance Ca2+-activated K+ current in patients with chronic atrial fibrillation[J]. Med Sci Monit, 2018, 24: 3011-3023. DOI: 10.12659/MSM.909684.
    [13]
    CSEPE T A, HANSEN B J, FEDOROV V V. Atrial fibrillation driver mechanisms: Insight from the isolated human heart[J]. Trends Cardiovasc Med, 2017, 27(1): 1-11. doi: 10.1016/j.tcm.2016.05.008
    [14]
    MASON F E, PRONTO J R D, ALHUSSINI K, et al. Cellular and mitochondrial mechanisms of atrial fibrillation[J]. Basic Res Cardiol, 2020, 115(6): 72. doi: 10.1007/s00395-020-00827-7
    [15]
    NATTEL S, HEIJMAN J, ZHOU L P, et al. Molecular basis of atrial fibrillation pathophysiology and therapy: A translational perspective[J]. Circ Res, 2020, 127(1): 51-72. doi: 10.1161/CIRCRESAHA.120.316363
    [16]
    WIJESURENDRA R S, CASADEI B. Mechanisms of atrial fibrillation[J]. Heart, 2019, 105(24): 1860-1867. doi: 10.1136/heartjnl-2018-314267
    [17]
    RAHM A K, GRAMLICH D, WIEDER T, et al. Trigger-specific remodeling of K(Ca)2 potassium channels in models of atrial fibrillation[J]. Pharmgenomics Pers Med, 2021, 14: 579-590. DOI: 10.2147/PGPM.S290291.
    [18]
    NAKAMURA T, TSUJITA K. Current trends and future perspectives for heart failure treatment leveraging cGMP modifiers and the practical effector PKG[J]. J Cardiol, 2021, 78(4): 261-268. doi: 10.1016/j.jjcc.2021.03.004
    [19]
    CHAO Y C, SURDO N C, PANTANO S, et al. Imaging cAMP nanodomains in the heart[J]. Biochem Soc Trans, 2019, 47(5): 1383-1392.
    [20]
    EZEANI M, PRABHU S. Pathophysiology and therapeutic relevance of PI3K (p110α) protein in atrial fibrillation: A non-interventional molecular therapy strategy[J]. Pharmacol Res, 2021, 165: 105415. DOI: 10.1016/j.phrs.2020.105415.
    [21]
    XUE X F, LING X Y, XI W, et al. Exogenous hydrogen sulfide reduces atrial remodeling and atrial fibrillation induced by diabetes mellitus via activation of the PI3K/Akt/eNOS pathway[J]. Mol Med Rep, 2020, 22(3): 1759-1766. doi: 10.3892/mmr.2020.11291
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(5)  / Tables(2)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (383) PDF downloads(15) Cited by()
    Proportional views
    Related

    Address：No. 287, Changhuai Road, Bengbu, Anhui Province, 233004, P.R.ChinaphoneNo：0552-3066635； 0552-3051890Email：zhqkyx@163.com

    Copyright © Chinese Journal of General Practice皖ICP备2020018345号-2

    Supported by: Beijing Renhe Information Technology Co., Ltd.    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return