Volume 20 Issue 5
May  2022
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Article Navigation >  Chinese Journal of General Practice  > 2022  >  20(5): 756-760
WANG Chao-chao, CHENG Zhe, CHANG Xue-lian, WANG Bang, WU Juan, HE Shi-wei, WANG Da-wei. Expression characteristics of tumor necrosis factor alpha inducible protein 3 in glioma and its effect on invasion and migration of glioma cell[J]. Chinese Journal of General Practice, 2022, 20(5): 756-760. doi: 10.16766/j.cnki.issn.1674-4152.002447
Citation: WANG Chao-chao, CHENG Zhe, CHANG Xue-lian, WANG Bang, WU Juan, HE Shi-wei, WANG Da-wei. Expression characteristics of tumor necrosis factor alpha inducible protein 3 in glioma and its effect on invasion and migration of glioma cell[J]. Chinese Journal of General Practice, 2022, 20(5): 756-760. doi: 10.16766/j.cnki.issn.1674-4152.002447
shu

Expression characteristics of tumor necrosis factor alpha inducible protein 3 in glioma and its effect on invasion and migration of glioma cell

doi: 10.16766/j.cnki.issn.1674-4152.002447
  • 1.

    Department of Neurosurgery, the Second Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233000, China

Funds:

 1908085QH334

 KJ2018A1016

 Byycx20033

  • Received Date: 2021-10-20
    Available Online: 2022-09-05
    Abstract
  •   Objective  To investigate the expression of tumor necrosis factor alpha inducible protein 3 (TNFAIP3) in gliomas and its effect on the invasion and migration of glioma cells.  Methods  Real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of TNFAIP3 in normal human astrocytes NHA and glioma cell lines U87, U251, SNB19, T98 and LN229, respectively. U87 and SNB19 were used as experiments subjects. Lipofectamine 2000-mediated small interference RNA (siRNA) was used to transfect U87 and SNB19 to down-regulate the expression of TNFAIP3. The effect of down-regulated TNFAIP3 on the invasion of U87 and SNB19 was detected by Transwell, and the migration was detected by scratch test.  Results  Compared with NHA (0.144±0.008), the expression of TNFAIP3 in U87 (1.380±0.066), U251 (0.663±0.062), SNB19 (1.405±0.032), T98 (1.002±0.068) and LN229 (0.667±0.027) were increased (all P < 0.05). Si-TNFAIP3 transfection into U87 and SNB19 could down-regulate the expression of TNFAIP3 and continue to culture for 24 hours, the invasion and migration ability of U87 and SNB19 were decreased (all P < 0.05).  Conclusion  TNFAIP3 is highly expressed in glioma, which can promote the invasion and migration of U87 and SNB19, and si-TNFAIP3 can inhibit the invasion and migration of glioma cells.

     

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