Volume 20 Issue 5
May  2022
Turn off MathJax
Article Contents
Article Navigation >  Chinese Journal of General Practice  > 2022  >  20(5): 828-831
WANG Mei, ZHANG Lin, CUI Fa-cai, WU Xiao-yu. The expression level of serum miR-96 and miR-424-5p in patients with colorectal cancer and its clinical significance[J]. Chinese Journal of General Practice, 2022, 20(5): 828-831. doi: 10.16766/j.cnki.issn.1674-4152.002465
Citation: WANG Mei, ZHANG Lin, CUI Fa-cai, WU Xiao-yu. The expression level of serum miR-96 and miR-424-5p in patients with colorectal cancer and its clinical significance[J]. Chinese Journal of General Practice, 2022, 20(5): 828-831. doi: 10.16766/j.cnki.issn.1674-4152.002465
shu

The expression level of serum miR-96 and miR-424-5p in patients with colorectal cancer and its clinical significance

doi: 10.16766/j.cnki.issn.1674-4152.002465
  • 1.

    Department of Clinical Laboratory, Henan Provincial Veteran's Hospital, Xinxiang, Henan 453003, China

Funds:

 201702220

  • Received Date: 2021-09-11
    Available Online: 2022-09-05
    Abstract
  •   Objective  To investigate the relationship between the expression of serum miR-96 and miR-424-5p and the clinicopathological characteristics and prognosis of patients with colorectal cancer (CRC), as well as explore the diagnostic value of serum miR-96 and miR-424-5p detection in CRC.  Methods  The serum samples of 120 patients with CRC, 40 patients with colorectal adenomas (CRAs) and 40 healthy controls from January to December 2015 in Henan Provincial People's Hospital were collected. The expression levels of miR-96 and miR-424-5p were detected by real-time quantitative PCR (RT-PCR). The relationship between the expression of miR-96 and miR-424-5p and the clinicopathological characteristics or the prognosis of patients with CRC was analysed. The receiver operating characteristic (ROC) curves were constructed to evaluate the diagnostic efficacy of serum miR-96 and miR-424-5p.  Results  The expression levels of serum miR-96 and miR-424-5p in patients with CRC were significantly higher than those in patients with CRA or healthy controls, with statistically significant differences (all P < 0.05). The expression level of serum miR-96 in patients with TNM stage Ⅲ/Ⅳ, low differentiation and distant metastasis was higher than those in patients with TNM Ⅰ/Ⅱ, moderate or high differentiation and without distant metastasis (all P < 0.01). The expression level of serum miR-424-5p in patients with TNM stage Ⅲ/Ⅳ was higher than that in patients with TNM Ⅰ/Ⅱ (all P < 0.01). The 5-year survival rate and mean survival time of patients with high expression of serum miR-96 and miR-424-5p were lower than those with low expression of serum miR-96 and miR-424-5p (all P < 0.01). The area under the curve (AUC) and specificity of miR-96 versus miR-424-5p in CRC were 0.727 and 71.2% versus 0.720 and 86.2%, respectively. The combined detection of AUC or specificity was 0.780 and 93.7%, which was greater than any single detection (all P < 0.05).  Conclusion  The increased expression levels of serum miR-96 and miR-424-5p were correlated with the occurrence, development and poor prognosis of CRC. The combined detection of serum miR-96 and miR-424-5p has significant reference value for the diagnosis of CRC.

     

    • FullText(HTML)
  • loading
    • References(19)
  • [1]
    BRAY F, FERLAY J, SOERJOMATARAM I, et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2018, 68: 394-424. doi: 10.3322/caac.21492
    [2]
    SIEGEL R L, MILLER K D, GODING SAUER A, et al. Colorectal cancer statistics, 2020[J]. CA Cancer J Clin, 2020, 70: 145-164. doi: 10.3322/caac.21601
    [3]
    LU T X, ROTHENBERG M E. MicroRNA[J]. J Allergy Clin Immunol, 2018, 141(4): 1202-1207. doi: 10.1016/j.jaci.2017.08.034
    [4]
    RAPADO-GONZÁLEZ Ó ÁLVAREZ-CASTRO A, LÓPEZ-LÓPEZ R, et al. Circulating microRNAs as promising biomarkers in colorectal cancer[J]. Cancers (Basel), 2019, 11(7): 898. doi: 10.3390/cancers11070898
    [5]
    SUN Y, LIU Y, COGDELL D, et al. Examining plasma microRNA markers for colorectal cancer at different stages[J]. Oncotarget, 2016, 7(10): 11434-11449. doi: 10.18632/oncotarget.7196
    [6]
    HE P Y, YIP W K, JABAR M F, et al. Effect of the miR-96-5p inhibitor and mimic on the migration and invasion of the SW480-7 colorectal cancer cell line[J]. Oncol Lett, 2019, 18(2): 1949-1960.
    [7]
    胡思锋, 王庆光, 李耀锋, 等. 微小RNA-96对结直肠癌细胞迁移及侵袭的影响[J]. 中华实验外科杂志, 2019, 36(1): 83-85. doi: 10.3760/cma.j.issn.1001-9030.2019.01.026

    HU S F, WANG Q G, LI Y F, et al. Effect of miR-96 on migration and invasion of colorectal cancer cells[J]. Chinese Journal of Experimental Surgery, 2019, 36(1): 83-85. doi: 10.3760/cma.j.issn.1001-9030.2019.01.026
    [8]
    GE T, XIANG P, MAO H, et al. Inhibition of miR-96 enhances the sensitivity of colorectal cancer cells to oxaliplatin by targeting TPM1[J]. Exp Ther Med, 2020, 20(3): 2134-2140.
    [9]
    DASTMALCHI N, HOSSEINPOURFEIZI M A, KHOJASTEH S M B, et al. Tumor suppressive activity of miR-424-5p in breast cancer cells through targeting PD-L1 and modulating PTEN/PI3K/AKT/mTOR signaling pathway[J]. Life Sci, 2020, 259: 118239. doi: 10.1016/j.lfs.2020.118239
    [10]
    MA L L, LIANG L, ZHOU D, et al. Tumor suppressor miR-424-5p abrogates ferroptosis in ovarian cancer through targeting ACSL4[J]. Neoplasma, 2020, 8: 200707N705.
    [11]
    ZHAO C, ZHAO F, CHEN H, et al. microRNA-424-5p inhibits the proliferation, migration, and invasion of nasopharyngeal carcinoma cells by decreasing AKT3 expression[J]. Braz J Med Biol Res, 2020, 53(7): e9029. doi: 10.1590/1414-431x20209029
    [12]
    王红, 曹梦迪, 刘成成, 等. 中国人群结直肠癌疾病负担: 近年是否有变?[J]. 中华流行病学杂志, 2020, 41(10): 1633-1642. doi: 10.3760/cma.j.cn112338-20200306-00273

    WANG H, CAO M D, LIU C C, et al. Disease burden of colorectal cancer in China: Any changes in recent years?[J]. Chinese Journal of Epidemiology, 2020, 41(10): 1633-1642. doi: 10.3760/cma.j.cn112338-20200306-00273
    [13]
    吴春晓, 顾凯, 龚杨明, 等. 2015年中国结直肠癌发病和死亡情况分析[J]. 中国癌症杂志, 2020, 30(4): 241-245. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGAZ202004001.htm

    WU C X, GU K, GONG Y M, et al. Analysis of incidence and mortality of colorectal cancer in China, 2015[J]. China Oncology, 2020, 30(4): 241-245. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGAZ202004001.htm
    [14]
    LONG J L, HE Q L, YIN YT, et al. The effect of miRNA and autophagy on colorectal cancer[J]. Cell Prolif, 2020, 53(10): e12900.
    [15]
    YIN X L, CHAI Z T, SUN X T, et al. Overexpression of microRNA-96 is associated with poor prognosis and promotes proliferation, migration and invasion in cholangiocarcinoma cells via MTSS1[J]. Exp Ther Med, 2020, 19(4): 2757-2765.
    [16]
    NING S F, LIU H Z, GAO B, et al. miR-155, miR-96 and miR-99a as potential diagnostic and prognostic tools for the clinical management of hepatocellular carcinoma[J]. Oncol Lett, 2019, 18(3): 3381-3387.
    [17]
    CHENG Z, SHU H S, CUI Y, et al. MiR-424-5p inhibits proliferation, invasion and promotes apoptosis and predicts good prognosis in glioma by directly targeting BFAR[J]. Pathol Oncol Res, 2020, 26(4): 2327-2335. doi: 10.1007/s12253-020-00831-1
    [18]
    YUE C F, CHEN J R, LI Z Y, et al. microRNA-96 promotes occurrence and progression of colorectal cancer via regulation of the AMPKα2-FTO-m6A/MYC axis[J]. J Exp Clin Cancer Res, 2020, 39(1): 240. doi: 10.1186/s13046-020-01731-7
    [19]
    SAHAMI-FARD M H, KHEIRANDISH S, SHEIKHHA M H. Expression levels of miR-143-3p and miR-424-5p in colorectal cancer and their clinical significance[J]. Cancer Biomark, 2019, 24(3): 291-297. doi: 10.3233/CBM-182171
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(3)  / Tables(1)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (171) PDF downloads(3) Cited by()
    Proportional views
    Related

    Address：No. 287, Changhuai Road, Bengbu, Anhui Province, 233004, P.R.ChinaphoneNo：0552-3066635； 0552-3051890Email：zhqkyx@163.com

    Copyright © Chinese Journal of General Practice皖ICP备2020018345号-2

    Supported by: Beijing Renhe Information Technology Co., Ltd.    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return