Volume 20 Issue 7
Jul.  2022
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Article Navigation >  Chinese Journal of General Practice  > 2022  >  20(7): 1126-1130
ZHOU Chao-rui, MA Wei-bin, YE Xiao-qian, CHEN Na, LI Ming-juan. Study on the inhibitory effect of propofol on upregulation of DR5 expression based on mouse melanoma lung metastasis model[J]. Chinese Journal of General Practice, 2022, 20(7): 1126-1130. doi: 10.16766/j.cnki.issn.1674-4152.002539
Citation: ZHOU Chao-rui, MA Wei-bin, YE Xiao-qian, CHEN Na, LI Ming-juan. Study on the inhibitory effect of propofol on upregulation of DR5 expression based on mouse melanoma lung metastasis model[J]. Chinese Journal of General Practice, 2022, 20(7): 1126-1130. doi: 10.16766/j.cnki.issn.1674-4152.002539
shu

Study on the inhibitory effect of propofol on upregulation of DR5 expression based on mouse melanoma lung metastasis model

doi: 10.16766/j.cnki.issn.1674-4152.002539
  • 1.

    Department of Anesthesiology, China Coast Guard Hospital of the People's Armed

Funds:

 LGD21H300001

  • Received Date: 2021-10-18
    Available Online: 2022-09-23
    Abstract
  •   Objective  Melanoma is a skin cancer caused by a malignancy of melanocytes. The incidence of melanoma is rapidly increasing worldwide, thus resulting in public health problems. Surgical resection is still the main method of early treatment. As a commonly used intravenous general anaesthesia drug, propofol has been reported to have a certain inhibitory effect on cell proliferation. This study aims to explore the inhibitory effect of propofol on lung metastasis of B16F10 melanoma in mice to provide a theoretical basis for propofol to be more suitable for surgical anaesthesia of malignant melanoma.  Methods  Cell viability was measured by CCK-8 assay. Flow cytometry was used to detect the apoptotic rate and Western blotting was used to detect the expression of DR5. In the in-vivo experiment, tumour cells were injected into the caudal vein to establish a lung metastatic model. Twenty-four male C57BL/6J mice were divided into 4 groups by random number table method, namely normal control group, melanoma lung metastasis model group, propofol 50 mg/kg, 10 mg/kg groups, 6 mice in each group. The effect of intraperitoneal injection of propofol on tumour metastatic nodules in the lung tissue of mice was observed.  Results  In the in-vitro experiment, the relative proliferation capacity (490 nm OD value) of cells in the control group and 10 μmol/L, 50 μmol/L, 100 μmol/L groups at 72 h were 0.91±0.03, 0.81±0.02, 0.71±0.02 and 0.61±0.02, respectively. The apoptotic rates of B16F10 cells treated with 100 μmol/L propofol were (8.09±1.01) %, (26.04±1.10) %, (32.94±1.30) % at 24, 48 and 72 h, respectively. In the in-vivo experiment, intraperitoneal injection of propofol at a dose of 50 mg/kg could significantly inhibit the number of nodules in lung metastatic melanoma and the immunohistochemical results showed that the expression of DR5 in lung tissue significantly increased in the propofol group.  Conclusion  Propofol could inhibit lung metastatic melanoma in mice and the mechanism may be related to the up-regulation of DR5 protein expression.

     

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