Volume 20 Issue 7
Jul.  2022
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Article Navigation >  Chinese Journal of General Practice  > 2022  >  20(7): 1151-1154
WANG Qin, LIN Ping, REN Qian, FENG Li, XIE Xiao-feng, ZHANG Bin, GAO Kang-lu. Serum AMPK-α mRNA, SIRT1 and GDF-8 levels in patients with sarcopenia[J]. Chinese Journal of General Practice, 2022, 20(7): 1151-1154. doi: 10.16766/j.cnki.issn.1674-4152.002545
Citation: WANG Qin, LIN Ping, REN Qian, FENG Li, XIE Xiao-feng, ZHANG Bin, GAO Kang-lu. Serum AMPK-α mRNA, SIRT1 and GDF-8 levels in patients with sarcopenia[J]. Chinese Journal of General Practice, 2022, 20(7): 1151-1154. doi: 10.16766/j.cnki.issn.1674-4152.002545
shu

Serum AMPK-α mRNA, SIRT1 and GDF-8 levels in patients with sarcopenia

doi: 10.16766/j.cnki.issn.1674-4152.002545

  • Department of the Geriatrics, Hangzhou Third People's Hospital, Hangzhou, Zhejiang 310009, China

Funds:

 2019KY502

 2018A29

 2018Z05

  • Received Date: 2021-04-14
    Available Online: 2022-09-23
    Abstract
  •   Objective  To investigate the level of serum AMP-activated protein kinase-α mRNA (AMPK-α mRNA), silent mating type information regulation 2 homolog-1 (SIRT1) and growth differentiation factor-8 (GDF-8) in patients with sarcopenia.  Methods  A total of 81 elderly patients treated in Hangzhou Third People's Hospital from January 2019 to June 2020 were selected. According to appendicular skeletal mass index (ASMI), grip strength and walking speed, the patients were divided into sarcopenia group (41 cases) and non-sarcopenia group (40 cases). The level of AMPK-α mRNA, SIRT1 and GDF-8 in serum were detected. The correlation was analyzed by Pearson and Spearman correlation. The diagnostic value of GDF-8 was analyzed by ROC curve.  Results  The data of several indexes of patients in sarcopenia group and non sarcopenia group were as follows [AMPK-α mRNA: 0.26 (0.24, 0.29) vs. 0.29 (0.27, 0.32), SIRT1 (ng/L): 582.68±35.92 vs. 646.50±39.08, GDF-8 (μg/L): 20.97±5.10 vs. 14.76±1.59], the difference was statistically significant (all P < 0.01). SIRT1 and AMPK-α mRNA were negatively correlated with GDF-8 (P < 0.001, P=0.001). The grip strength was positively correlated with AMPK-α mRNA and SIRT1 (P=0.028, P < 0.001), and was negatively correlated with GDF-8 (P=0.001). The critical value of GDF-8 for the diagnosis of sarcopenia was 17.25 μg/L, and AUC was 0.884 (P=0.040).  Conclusion  AMPK-α mRNA and SIRT1 are decreased and GDF-8 is increased in sarcopenia group. GDF-8 can be used as an auxiliary reference index for the diagnosis of sarcopenia.

     

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