Expression and significance of CD8<sup>+</sup> T cells and NK cells in peripheral blood of patients with cervical cancer

HATILA Tuerxun; MIKRBAN Tursun; LIU Qian; LI Jin-qiu; ALFIRA Abdujapar; AYSHAMGUL Hasim

doi:10.16766/j.cnki.issn.1674-4152.002581

Volume 20 Issue 8

Aug. 2022

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Article Navigation > Chinese Journal of General Practice > 2022 > 20(8): 1299-1301

HATILA Tuerxun, MIKRBAN Tursun, LIU Qian, LI Jin-qiu, ALFIRA Abdujapar, AYSHAMGUL Hasim. Expression and significance of CD8+ T cells and NK cells in peripheral blood of patients with cervical cancer[J]. Chinese Journal of General Practice, 2022, 20(8): 1299-1301. doi: 10.16766/j.cnki.issn.1674-4152.002581

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HATILA Tuerxun, MIKRBAN Tursun, LIU Qian, LI Jin-qiu, ALFIRA Abdujapar, AYSHAMGUL Hasim. Expression and significance of CD8⁺ T cells and NK cells in peripheral blood of patients with cervical cancer[J]. Chinese Journal of General Practice, 2022, 20(8): 1299-1301. doi: 10.16766/j.cnki.issn.1674-4152.002581

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Expression and significance of CD8⁺ T cells and NK cells in peripheral blood of patients with cervical cancer

doi: 10.16766/j.cnki.issn.1674-4152.002581

1.
Department of Pathology, School of Basic Medicine, Xinjiang Medical University, Xinjiang Key Laboratory of Molecular Biology for Endemic Diseases, Urumqi, Xinjiang 830054, China

Funds:

2018D01C151

XJ2020G186

Received Date: 2021-12-21
Available Online: 2022-09-26

Abstract

Abstract

Objective To analyze the expression levels and clinical significance of natural killer cells (NK), CD8⁺ T cells and immune checkpoint molecules TIGIT, PD1 and PDL1 in peripheral blood of patients with cervical cancer in Xinjiang. Methods Fourteen cervical cancer patients and fourteen healthy people admitted to the First Affiliated Hospital of Xinjiang Medical University from September 2020 to February 2021 were divided into cervical cancer group and control group. Flow cytometry was used to detect the distribution ratio of CD8⁺ T cells and NK cells in peripheral blood and the expression levels of immune checkpoint molecules, TIGIT, PD1 and PDL1. Results The proportion of CD8⁺ T cells in peripheral blood of cervical cancer patients [(22.66±3.79)%] was lower than that of the control group [(28.30±8.61)%, P < 0.01], however, the proportion of NK cells in peripheral blood of cervical cancer patients has no difference compared with the control group (P>0.05). The level of PDL1 in NK cells from peripheral blood of cervical cancer patients [(5.40±4.44)%] was lower than that of the control group [(21.03±16.65)%], the difference was statistically significant (P < 0.01). However, the expression of TIGIT and PD1 in the peripheral blood of cervical cancer patient were not significantly different from those of the control group (all P>0.05). The proportions of TIGIT, PD1 and PDL1 on CD8⁺ T cells in peripheral blood of cervical cancer patients were not significantly different from those of the control group (P>0.05). Conclusion Detection of NK cells, CD8⁺ T cells and immune checkpoint molecules in peripheral blood of patients with cervical cancer can monitor the immune status of cervical cancer patients, which providing reference for clinical treatment.
- Cervical cancer,
- CD8⁺ T cells,
- Natural killer cells,
- Immune checkpoint

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[1]	王海波, 崔薇, 杨玮丽, 等. 基于多序列MRI影像组学预测早期宫颈癌淋巴血管侵犯的研究[J]. 中华全科医学, 2021, 19(12): 2088-2092. doi: 10.16766/j.cnki.issn.1674-4152.002244 WANG H B, CUI W, YANG W L, et al. Multi-sequence MRI-based radiomics predicting lymph-vascular space invasion in early-stage cervical cancer[J]. Chinese Journal of General Practice, 2021, 19(12): 2088-2092. doi: 10.16766/j.cnki.issn.1674-4152.002244
[2]	YUTUAN W, SHUANG Y, SHYAMAL G, et al. Clinical significance of peripheral blood and tumor tissue lymphocyte subsets in cervical cancer patients[J]. BMC Cancer, 2020, 20(1): 173. doi: 10.1186/s12885-020-6633-x
[3]	吕玥茜, 李茵佳, 周素芳. 以程序性死亡受体-1为靶点的肝癌靶向治疗研究进展[J]. 重庆医科大学学报, 2021, 46(5): 630-634. https://www.cnki.com.cn/Article/CJFDTOTAL-ZQYK202105026.htm LYU Y Q, LI Y J, ZHOU S F. Advances in programmed cell death receptor-1 targeted therapy for liver cancer[J]. Journal of Chongqing Medical University, 2021, 46(5): 630-634. https://www.cnki.com.cn/Article/CJFDTOTAL-ZQYK202105026.htm
[4]	RAMB M, D0LPADY J, KULKARNL R, et al. Human papillomavirus(HPV) oncoprotein E₆ facilitates Calcineurin-Nuclear factor for activated T cells2(NFAT2)signaling to promote cellular proliferation in cervical cell carcinoma[J]. Exp Cell Res, 2018, 362 (1): 132-141. doi: 10.1016/j.yexcr.2017.11.010
[5]	MC CORMACK S E, CRUZ C R Y, WRIGHT K E, et al. Human papilloma virus-specific T cells can be generated from na_1: Ve T cells for use as an immunotherapeutic strategy for immunocompromised patients[J]. Cytotherapy, 2018, 20(3): 385-393. doi: 10.1016/j.jcyt.2017.11.010
[6]	DE RUITER EMMA J, DE RREINOUT H, et al. Digital pathology-aided assessment of tumor-infiltrating T lymphocytes in advanced stage, HPV-negative head and neck tumors[J]. CⅡ, 2020, 69(4): 581-591.
[7]	PAVER E C, CURRIE A M, GUPTA R, et al. Human papilloma virus related squamous cell carcinomas of the head and neck: Diagnosis, clinical implications and detection of HPV[J]. Pathology, 2020, 52(2): 179-191. doi: 10.1016/j.pathol.2019.10.008
[8]	TANG Y T, XU Q, HU L, et al. Tumor microenvironment-derived R-spondins enhance anti-tumor immunity to suppress tumor growth and sensitize for immune checkpoint blockade therapy[J]. Cancer Discov, 2021, 11(12): 3142-3157. doi: 10.1158/2159-8290.CD-20-0833
[9]	VUL T H, TRAN H T D, NGUYEN B T, et al. Community-based screening for cervical cancer using visual inspection with acetic acid: Results and lessons learned from a pilot study in vietnam[J]. Public Health Manag Pract, 2018, 24(Suppl 2): S3-S8.
[10]	王太成, 宋文渊, 赵红岩, 等. 肝细胞肝癌患者血清前蛋白水平和自然杀伤细胞比率与肝切除术后发生急性肝衰竭的相关性[J]. 中华实用诊断与治疗杂志, 2021, 35(7): 676-679. https://www.cnki.com.cn/Article/CJFDTOTAL-HNZD202107007.htm WANG T C, SONG W Y, ZHAO H Y, et al. Correlations of serum prealbumin level and natural killer cell ratio with acute liver failure after hepatectomy in patients with hepatocellular carcinoma[J]. Journal of Chinese Practical Diagnosis and Therapy, 2021, 35(7): 676-679. https://www.cnki.com.cn/Article/CJFDTOTAL-HNZD202107007.htm
[11]	秦林, 林涛, 沈炼桔, 等. IL-2联合IL-15提高肾母细胞瘤患儿外周血NK细胞对肿瘤细胞的杀伤作用[J]. 中华小儿外科杂志, 2018, 39(5): 345-347. doi: 10.3760/cma.j.issn.0253-3006.2018.05.006 QIN L, LIN T, SHEN L J, et al. Interleukin-2 and Interleukin-15 enhance the cytotoxicity of NK cell against tumor in peripheral blood of Wilms tumor children[J]. Chinese Journal of Pediatric Surgery, 2018, 39(5): 345-347. doi: 10.3760/cma.j.issn.0253-3006.2018.05.006
[12]	DMITRIJ O, STEVEN D, JESSICA W, et al. Transcriptome profiling identifies TIGIT as a marker of T-cell exhaustion in liver cancer[J]. Hepatology, 2021, 73(4): 1399-1418. doi: 10.1002/hep.31466
[13]	TANG W W, PAN X X, HAN D, et al. Clinical significance of CD8T cell immunoreceptor with Ig and ITIMdomains in locally advanced gastric cancer treated with SOX regimen after D2gastrectomy[J/CD]. Onco Immunology, 2019, 8(6): e1593807. DOI: 10.1080/2162402X.2019.1593807.
[14]	SEAN J J, MORGAN A D, STEVE W T, et al. Analysis of tumor-infiltrating NK and T cells highlights IL-15 stimulation and TIGIT blockade as a combination immunotherapy strategy for soft tissue sarcomas[J]. J Immunother Cancer, 2020, 8(2): e001355. DOI: 10.1136/jitc-2020-001355.
[15]	GUILLEREY C, HARJUNPÄÄ H, CARRIÉ N, et al. TIGIT immune check-point blockade restores CD8⁺ T-cell immunity against multiple mye-loma[J]. Blood, 2018, 132(16): 1689-1694.
[16]	CONCHA-BENAVENTE F, KANSY B, MOSKOVITZ J, et al. PD-Ll mediates dysfunction in activated PD-I (+) NK cells in head and neck cancer patients[J]. Cancer Immunol Res, 2018, 6(12): 1548-1560.

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Expression and significance of CD8⁺ T cells and NK cells in peripheral blood of patients with cervical cancer

doi: 10.16766/j.cnki.issn.1674-4152.002581