Volume 20 Issue 8
Aug.  2022
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Article Navigation >  Chinese Journal of General Practice  > 2022  >  20(8): 1346-1349
LIU Xuan-shi, HAO Chan-juan, XU Li-xin, LI Wei. Effects of tobacco exposure during pregnancy on pregnant women and foetuses based on placental gene expression profiling[J]. Chinese Journal of General Practice, 2022, 20(8): 1346-1349. doi: 10.16766/j.cnki.issn.1674-4152.002593
Citation: LIU Xuan-shi, HAO Chan-juan, XU Li-xin, LI Wei. Effects of tobacco exposure during pregnancy on pregnant women and foetuses based on placental gene expression profiling[J]. Chinese Journal of General Practice, 2022, 20(8): 1346-1349. doi: 10.16766/j.cnki.issn.1674-4152.002593
shu

Effects of tobacco exposure during pregnancy on pregnant women and foetuses based on placental gene expression profiling

doi: 10.16766/j.cnki.issn.1674-4152.002593
  • 1.

    Beijing Key Laboratory for Genetics of Birth Defects, Beijing Paediatric Research Institute; MOE Key Laboratory of Major Diseases in Children; Genetics and Birth Defects Control Centre, Beijing Children' s Hospital, Capital Medical University, National Centre for Children' s Health, Beijing 100045, China

Funds:

 2016YFC1000306

 5222007

  • Received Date: 2021-12-15
    Abstract
  •   Objective  To expand the understanding of the molecular mechanisms underlying tobacco exposure during pregnancy on pregnant women and their foetuses.  Methods  Firstly, the NCBI GEO database and EBI ArrayExpress database were systematically searched, and two gene expression profile datasets from placental tissues of smoking and non-smoking pregnant women were selected. After integrating and correcting these datasets, various bioinformatics methods and models including differential expression, functional annotation, enrichment analysis and regression analysis were applied to study the effects of tobacco exposure on pregnant women and foetuses.  Results  Amongst 476 significantly differentially expressed genes (FDR < 0.5, |log2FC|>0.3), 317 were up-regulated and 159 were down-regulated. After functional annotations and enrichment analyses using KEGG, GO, Reactome and MSigDb, these genes mainly appeared in extracellular structure (P=1.10×10-28), extracellular matrix (P=3.44×10-25) and mesenchymal development (P=2.60×10-13). Regression analysis was conducted to study molecular markers of tobacco exposure. CDCA7L was the most significant gene correlated with cotinine level after adjusting for confounding factors (FDR=0.046).  Conclusion  The pathways and the possible molecular mechanisms that pregnant women and foetuses may be affected by tobacco exposure are explored and discussed, and the ideas for future molecular mechanism research are proposed.

     

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