Volume 20 Issue 11
Nov.  2022
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Article Navigation >  Chinese Journal of General Practice  > 2022  >  20(11): 1831-1835
LI Cai, XU Pan-pan, HU Jie, ZHU Kun, YE Yu-chen, ZHANG Ya-wei, ZHANG Chang-chun. Melatonin regulates Nrf2/ARE signal pathway to delay the degeneration of nucleus pulposus mesenchymal stem cells[J]. Chinese Journal of General Practice, 2022, 20(11): 1831-1835. doi: 10.16766/j.cnki.issn.1674-4152.002713
Citation: LI Cai, XU Pan-pan, HU Jie, ZHU Kun, YE Yu-chen, ZHANG Ya-wei, ZHANG Chang-chun. Melatonin regulates Nrf2/ARE signal pathway to delay the degeneration of nucleus pulposus mesenchymal stem cells[J]. Chinese Journal of General Practice, 2022, 20(11): 1831-1835. doi: 10.16766/j.cnki.issn.1674-4152.002713
shu

Melatonin regulates Nrf2/ARE signal pathway to delay the degeneration of nucleus pulposus mesenchymal stem cells

doi: 10.16766/j.cnki.issn.1674-4152.002713
  • 1.

    Department of Orthopaedics, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

Funds:

 1908085MC90

 KJ2021A0743

 Byycx20029

 bydc2021017

  • Received Date: 2022-02-06
    Available Online: 2022-12-30
    Abstract
  •   Objective  To explore the effect of melatonin on rat nucleus pulposus mesenchymal stem cells (NPMSCs) under oxidative stress and its regulatory mechanism.  Methods  Rat nucleus pulposus mesenchymal stem cells were isolated and purified by differential adhesion method. The state of cells was observed under the microscope to identify the differentiation potential of the third generation cells and cell surface immune markers. CCK-8 method was used to detect the effect of different concentrations of hydrogen peroxide and melatonin on the activity of NPMSCs in rats to determine the optimal concentration and experimental grouping. The level of reactive oxygen species in NPMSCs were detected by immunofluorescence and flow cytometry. The expressions of extracellular matrix marker proteins (collagen Ⅱ and glycosaminoglycan) and key proteins (Nrf2, HO-1) in Nrf2/ARE signaling pathway were analyzed by Western blotting.  Results  Melatonin protected the activity of NPMSCs under oxidative stress, and the protective effect of 1 μmol/L concentration was the most obvious (0.717±0.018, t=7.102, P < 0.01). Melatonin could inhibit the level of reactive oxygen species induced by H2O2 (positive rate: 70.0% vs. 32.7%). In addition, melatonin significantly increased the activities of Nrf2 (1.925±0.024, t=13.150, P < 0.01) and HO-1 (1.605±0.019, t=12.940, P < 0.01), and improved the function of NPMSCs. After treatment with melatonin, the expression of Collagen Ⅱ (0.850±0.010, t=25.200, P < 0.01) and glycosaminoglycan (0.335±0.013, t=10.640, P < 0.01) increased.  Conclusion  Melatonin affects the biological characteristics of NPMSCs degeneration by regulating Nrf2/ARE signal pathway.

     

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