Volume 20 Issue 11
Nov.  2022
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Article Navigation >  Chinese Journal of General Practice  > 2022  >  20(11): 1841-1844
WANG Yan, WANG Ling-ling, WANG Cai-zhi. The Relationship between HPV E6/E7 mRNA, vaginal environment and cervical diseases[J]. Chinese Journal of General Practice, 2022, 20(11): 1841-1844. doi: 10.16766/j.cnki.issn.1674-4152.002715
Citation: WANG Yan, WANG Ling-ling, WANG Cai-zhi. The Relationship between HPV E6/E7 mRNA, vaginal environment and cervical diseases[J]. Chinese Journal of General Practice, 2022, 20(11): 1841-1844. doi: 10.16766/j.cnki.issn.1674-4152.002715
shu

The Relationship between HPV E6/E7 mRNA, vaginal environment and cervical diseases

doi: 10.16766/j.cnki.issn.1674-4152.002715

  • Department of Obstetrics and Gynecology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

Funds:

 KJ2021A0754

 BYKY2019120ZD

  • Received Date: 2022-04-06
    Available Online: 2022-12-30
    Abstract
  •   Objective  To investigate the relationship between the expression of HPV E6/E7 mRNA, vaginal microecological abnormalities and cervical lesions in women of childbearing age.  Methods  A total of 1 245 patients of reproductive age who were admitted to the outpatient and inpatient Departments of Obstetrics and Gynecology of the First Affiliated Hospital of Bengbu Medical College for cervical cancer screening from December 2019 to August 2021 were enrolled. All patients were examined by colposcopy. According to the results of colposcopy pathology, there were 290 cases in cervical intraepithelial neoplasia (CIN) Ⅰ group, 360 cases in CIN Ⅱ group, 465 cases in CIN Ⅲ group, 130 cases in cervical squamous cell carcinoma group and 150 healthy women in control group. By detecting the expression of HPV E6/E7 mRNA and the pathogen of vaginal secretions, the relationship between HPV E6/E7 mRNA and cervical diseases was compared.  Results  There was a statistically significant difference in vaginal cleanliness among the groups (χ2=46.123, P < 0.05), among which the detection rate of vaginal cleanliness Ⅲ-Ⅳ in the control group < CIN Ⅰ group < CIN Ⅱ group < CIN Ⅲ group < CECS group. The positive detection rate of Gardnerella in each group was statistically significant (χ2=39.404, P < 0.05). There was no significant difference in the positive rates of candida and trichomonas in each group (P>0.05). The positive detection rate of HPV E6/E7 mRNA in each group was statistically significant (χ2=287.386, P < 0.05). The positive detection rates of HPV E6/E7 mRNA in CIN Ⅰ, CIN Ⅱ, CIN Ⅲ, CECS and control groups were 50.7%, 81.9%, 76.1%, 79.2% and 13.3%, respectively. The positive expression rate of HPV E6/E7 mRNA in CIN Ⅰ, CIN Ⅱ, CIN Ⅲ and CESC groups was correlated with the rate of vaginal microecology disorder (P < 0.05). Logistic regression analysis showed that vaginal cleanliness (Ⅰ- Ⅱ) and lactobacillus were independent protective factors for HPV E6/E7 mRNA expression (OR < 1, all P < 0.05).  Conclusion  The increased positive rate of HPV E6/E7 mRNA expression, the increased detection rate of vaginal cleanliness (Ⅲ- Ⅳ), and the increased positive detection rate of Gardnerella are all high risk factors for predicting cervical cancer or cervical precancerous lesions.

     

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