Volume 20 Issue 11
Nov.  2022
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Article Navigation >  Chinese Journal of General Practice  > 2022  >  20(11): 1881-1883
LIN Yan-ling, WANG Ying, HUANG Qi, KANG Ling, XING Wei-zhen. Detection and clinical value of serum levels of miR-143-3p, miR-222-3p and AMH in patients with polycystic ovary syndrome[J]. Chinese Journal of General Practice, 2022, 20(11): 1881-1883. doi: 10.16766/j.cnki.issn.1674-4152.002726
Citation: LIN Yan-ling, WANG Ying, HUANG Qi, KANG Ling, XING Wei-zhen. Detection and clinical value of serum levels of miR-143-3p, miR-222-3p and AMH in patients with polycystic ovary syndrome[J]. Chinese Journal of General Practice, 2022, 20(11): 1881-1883. doi: 10.16766/j.cnki.issn.1674-4152.002726
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Detection and clinical value of serum levels of miR-143-3p, miR-222-3p and AMH in patients with polycystic ovary syndrome

doi: 10.16766/j.cnki.issn.1674-4152.002726

  • Department of Gynecology, Sanya Women and Children's Hospital Managed by Shanghai Children's Medical Center, Sanya, Hainan 572000, China

Funds:

 19A200193

 琼卫(2021)75号

  • Received Date: 2022-05-02
    Available Online: 2022-12-30
    Abstract
  •   Objective  To investigate the expression and clinical value of serum miR-143-3p, miR-222-3p levels combined with anti-Mullerian hormone (AMH) in polycystic ovary syndrome (PCOS).  Methods  Total 128 patients with PCOS (PCOS group) treated in Sanya Women and Children's Hospital from January 2018 to March 2022 and 50 healthy women (control group) were selected. According to insulin resistance index (HOMA-IR), PCOS patients were divided into insulin resistance group (n=83, HOMA-IR≥2.69) and non-insulin resistance group (n=45, HOMA-IR < 2.69). According to BMI, PCOS patients were divided into obesity group (n=40, BMI≥28) and non-obesity group (n=88, BMI < 28). According to the level of testosterone, they were divided into hyperandrogen group (n=71, testosterone≥0.7 ng/mL) and non-hyperandrogen group (n=57, testosterone < 0.7 ng/mL). The serum levels of miR-143-3p and miR-222-3p were detected by real-time quantitative PCR, and AMH was detected by electrochemiluminescence. The diagnostic value of miR-143-3p, miR-222-3p combined with AMH in PCOS were analyzed by receiver operating characteristic (ROC) curve. Pearson correlation analysis was used for correlation analysis.  Results  The serum levels of miR-143-3p, miR-222-3p and AMH in PCOS group were significantly higher than those in control group, those in obesity group was significantly higher than that in non-obesity group, those in hyperandrogen group was significantly higher than that in non-hyperandrogen group, and insulin resistance group was significantly higher than that in non-insulin resistance group, with statistical significance (all P < 0.001). ROC curve showed that the combined diagnosis of PCOS by miR-143-3p, miR-222-3p and AMH had the largest area under the curve (AUC=0.910, 95% CI: 0.852-0.974). The serum levels of miR-143-3p and miR-222-3p in PCOS patients were positively correlated with AMH (r=0.842, 0.795, all P < 0.001).  Conclusion  The serum levels of miR-143-3p, miR-222-3p and AMH were significantly increased in PCOS patients, and the detection of the three items were helpful for the diagnosis of PCOS.

     

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    • References(17)
  • [1]
    STENER-VICTORIN E, DENG Q L. Epigenetic inheritance of polycystic ovary syndrome-challenges and opportunities for treatment[J]. Nat Rev Endocrinol, 2021, 17(9): 521-533. doi: 10.1038/s41574-021-00517-x
    [2]
    SADEGHI H M, ADELI I, CALINA D, et al. Polycystic ovary syndrome: A comprehensive review of pathogenesis, management, and drug repurposing[J]. Int J Mol Sci, 2022, 23(2): 583. doi: 10.3390/ijms23020583
    [3]
    王昱欢, 丁奕岑, 蔡瑶雨, 等. 差异表达微RNA作为多囊卵巢综合征生物标志物的研究[J]. 上海交通大学学报(医学版), 2021, 41(11): 1429-1435. doi: 10.3969/j.issn.1674-8115.2021.11.005

    WANG Y H, DING Y C, CAI Y Y, et al. Study on differentially expressed microRNA as a biomarker of polycystic ovary syndrome[J]. Journal of Shanghai Jiaotong University (Medical Edition), 2021, 41(11): 1429-1435. doi: 10.3969/j.issn.1674-8115.2021.11.005
    [4]
    CAO J P, HUO P, CUI K Q, et al. Follicular fluid-derived exosomal miR-143-3p/miR-155-5p regulate follicular dysplasia by modulating glycolysis in granulosa cells in polycystic ovary syndrome[J]. Cell Commun Signal, 2022, 20(1): 61. doi: 10.1186/s12964-022-00876-6
    [5]
    YE H, LIU X J, HUI Y, et al. Downregulation of microRNA-222 reduces insulin resistance in rats with PCOS by inhibiting activation of the MAPK/ERK pathway via pten[J]. Mol Ther Nucleic Acids, 2020, 22(7): 733-741.
    [6]
    秦薇, 黄筱竑, 黄慧莲. HIF-1α、VEGF及AMH在多囊卵巢综合征中的表达及其临床意义[J]. 中华全科医学, 2021, 19(10): 1693-1696. doi: 10.16766/j.cnki.issn.1674-4152.002144

    QIN W, HUANG X H, HUANG H L. Expression and clinical significance of HIF-1α, VEGF and AMH in patients with polycystic ovary syndrome[J]. Chinese Journal of General Practice, 2021, 19(10): 1693-1696. doi: 10.16766/j.cnki.issn.1674-4152.002144
    [7]
    中华医学会妇产科学分会内分泌学组及指南专家组. 多囊卵巢综合征中国诊疗指南[J]. 中华妇产科杂志, 2018, 53(1): 2-6. https://www.cnki.com.cn/Article/CJFDTOTAL-SFCZ201810010.htm

    Endocrinology group and guideline expert group of Obstetrics and Gynecology branch of Chinese Medical Association. Chinese guidelines for diagnosis and treatment of polycystic ovary syndrome[J]. Chinese Journal of Obstetrics and Gynecology, 2018, 53(1): 2-6. https://www.cnki.com.cn/Article/CJFDTOTAL-SFCZ201810010.htm
    [8]
    FAROOQUI N, MAHA Q U. Role of some MiRNA affecting polycystic ovarian syndrome and SIRT1 levels[J]. J Pak Med Assoc, 2020, 70(5): 945.
    [9]
    WANG W, JI J, LI J, et al. Several critical genes and microRNAs associated with the development of polycystic ovary syndrome[J]. Ann Endocrinol (Paris), 2020, 81(1): 18-27. doi: 10.1016/j.ando.2019.10.002
    [10]
    杨东霞, 申儒霞, 李红梅, 等. 微小RNA在多囊卵巢综合征发病机制中的研究进展[J]. 中国生育健康杂志, 2022, 33(1): 81-83, 89. https://www.cnki.com.cn/Article/CJFDTOTAL-SYJK202201021.htm

    YANG D X, SHEN R X, LI H M, et al. Research progress of microRNA in the pathogenesis of polycystic ovary syndrome[J]. Chinese Journal of Reproductive Health, 2022, 33(1): 81-83, 89. https://www.cnki.com.cn/Article/CJFDTOTAL-SYJK202201021.htm
    [11]
    BUTLER A E, RAMACHANDRAN V, HAYAT S, et al. Expression of microRNA in follicular fluid in women with and without PCOS[J]. Sci Rep, 2019, 9(1): 16306. DOI: 10.1038/s41598-019-52856-5.
    [12]
    DU J, RUAN X Y, JIN F Y, et al. Abnormalities of early folliculogenesis and serum anti-müllerian hormone in chinese patients with polycystic ovary syndrome[J]. J Ovarian Res, 2021, 14(1): 36. doi: 10.1186/s13048-021-00786-0
    [13]
    ZHAO Y Y, PAN S H, LI Y Y, et al. Exosomal miR-143-3p derived from follicular fluid promotes granulosa cell apoptosis by targeting BMPR1A in polycystic ovary syndrome[J]. Sci Rep, 2022, 12(1): 4359. DOI: 10.1038/s41598-022-08423-6.
    [14]
    HUANG X L, SHE L P, LUO X M, et al. MiR-222 promotes the progression of polycystic ovary syndrome by targeting p27 Kip1[J]. Pathol Res Pract, 2019, 215(5): 918-923. doi: 10.1016/j.prp.2019.01.038
    [15]
    LI X J, WANG H, LU D Y, et al. Anti-müllerian hormone accelerates pathological process of insulin resistance in polycystic ovary syndrome patients[J]. Horm Metab Res, 2021, 53(8): 504-511. doi: 10.1055/a-1499-7718
    [16]
    CHEN Z, OU H X, WU H L, et al. Role of microRNA in the pathogenesis of polycystic ovary syndrome[J]. DNA Cell Biol, 2019, 38(8): 754-762. doi: 10.1089/dna.2019.4622
    [17]
    SHI X Y, PENG D, LIU Y F, et al. Advantages of serum anti-müllerian hormone as a marker for polycystic ovarian syndrome[J]. Lab Med, 2019, 50(3): 236-242. doi: 10.1093/labmed/lmy068
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