Volume 21 Issue 3
Mar.  2023
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Article Navigation >  Chinese Journal of General Practice  > 2023  >  21(3): 365-368
ZHUO Feixiang, ZHANG Zhen, CHEN Xin. Expression and significance of miR-124, NSE and S100B in neonatal hypoxic-ischemic encephalopathy[J]. Chinese Journal of General Practice, 2023, 21(3): 365-368. doi: 10.16766/j.cnki.issn.1674-4152.002884
Citation: ZHUO Feixiang, ZHANG Zhen, CHEN Xin. Expression and significance of miR-124, NSE and S100B in neonatal hypoxic-ischemic encephalopathy[J]. Chinese Journal of General Practice, 2023, 21(3): 365-368. doi: 10.16766/j.cnki.issn.1674-4152.002884
shu

Expression and significance of miR-124, NSE and S100B in neonatal hypoxic-ischemic encephalopathy

doi: 10.16766/j.cnki.issn.1674-4152.002884

  • Department of Pediatrics, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

Funds:

 KJ2021A0800

  • Received Date: 2022-06-20
    Available Online: 2023-04-19
    Abstract
  •   Objective  To investigate the expression levels of central neuron-specific protein (S100B), neuron-specific enolase (NSE) and miR-124 in neonatal hypoxic-ischemic encephalopathy (HIE) and to explore their diagnostic value.  Methods  A total of 100 full-term neonates in the First Affiliated Hospital of Bengbu Medical College from December 2020 to December 2021 were selected, including 50 neonates in the HIE group and 50 neonates in the healthy group. Umbilical cord blood was collected within 2 hours after birth, and the level of miR-124 was detected by real-time fluorescence quantification PCR (qRT-PCR); and femoral vein blood was collected from neonates within 6 hours after birth to detect S100B and NSE levels by ELASA, and the receiver operating characteristic (ROC) curve was constructed to evaluate the clinical significance of the above biomarkers in HIE.  Results  Compared to healthy neonates, the expression level of miR-124 was decreased in neonates with HIE (0.63±0.11 vs. 0.90±0.22), whereas the levels of S100B and NSE were significantly increased [0.45 (0.34, 0.78) μg/L vs. 0.33 (0.24, 0.50) μg/L, 39.30 (23.71, 67.48) μg/L vs. 21.16 (16.27, 27.25) μg/L], the differences were statistically significant (all P < 0.05), and there were statistically significant differences in miR-124, S100B and NSE in neonates with different HIE levels (all P < 0.05). ROC analysis results showed that the area under the curve AUCs of serum miR-124, S100B and NSE for HIE diagnosis were 0.897, 0.690 and 0.801, respectively, and the AUC of combined detection of miR-124, S100B and NSE for HIE diagnosis was 0.908, indicating the best diagnostic efficiency. The Neonatal Behavioral Neurological Assessment (NBNA) score of HIE was negatively correlated with S100B and NSE, and positively correlated with miR-124.  Conclusion  The expression level of miR-124 in umbilical cord blood of neonates with HIE is decreased, and miR-124 combined with S100B and NSE is of high diagnostic value for HIE.

     

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