Volume 21 Issue 3
Mar.  2023
Turn off MathJax
Article Contents
Article Navigation >  Chinese Journal of General Practice  > 2023  >  21(3): 393-395
JIANG Chuanyue, TAO Yafen, WU Yue, WANG Qianghua, WANG Xuegu, DING Biao, LI Xiang. Effects of stearic acid on the functions of L-type calcium channel in vascular smooth muscle cells[J]. Chinese Journal of General Practice, 2023, 21(3): 393-395. doi: 10.16766/j.cnki.issn.1674-4152.002891
Citation: JIANG Chuanyue, TAO Yafen, WU Yue, WANG Qianghua, WANG Xuegu, DING Biao, LI Xiang. Effects of stearic acid on the functions of L-type calcium channel in vascular smooth muscle cells[J]. Chinese Journal of General Practice, 2023, 21(3): 393-395. doi: 10.16766/j.cnki.issn.1674-4152.002891
shu

Effects of stearic acid on the functions of L-type calcium channel in vascular smooth muscle cells

doi: 10.16766/j.cnki.issn.1674-4152.002891
  • 1.

    Reproductive Medicine Center, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

Funds:

 2008085QH407

  • Received Date: 2022-07-12
    Available Online: 2023-04-19
    Abstract
  •   Objective  High-fat diet has adverse effects on cardiovascular health, but the mechanism remains unclear. This study aims to investigate the effect of stearic acid induced by high-fat diet on the function of L-type calcium channel in vascular smooth muscle cells(VSMCs).  Methods  Four week-old SD rats were randomly divided into control group (6 rats) and high-fat group (6 rats) using a lottery method, and fed with normal standard diet and high-fat diet (containing 45% fat) for 12 weeks, respectively. The commercial kits and rat femoral artery cannulation techniques were used to detect blood free fatty acids and rat blood pressures respectively. Calcium currents in rat mesenteric VSMCs was detected by patch clamp technique.  Results  Compared with control group, the free fatty acids [(0.667±0.027) mmol/L vs. (0.468±0.012) mmol/L, t=6.841, P < 0.001], systolic [(136.50±2.19) mmHg vs. (121.20±3.78) mmHg, t=3.507, P=0.006] and diastolic [(87.10±2.42) mmHg vs. (73.70±2.74) mmHg, t=3.660, P=0.004)] blood pressure was significantly increased in rats with high-fat diet. Patch-clamp results showed that the calcium channel currents were significantly increased in VSMCs of mesenteric artery of the rats with high-fat diet (the ratio of pA/pF at a membrane potential of 10 mV was -16.86±2.58 in control group and -23.80±2.15 in high-fat group, t=4.449, P < 0.001). In addition, stearic acid could significantly increase calcium channel currents (the pA/pF at 10 mV: -12.93±2.58 in 0 μmol/L stearic acid group; -18.10±2.15 in 30 μmol/L stearic acid group; t=4.097, P=0.001) while not affect the calcium channel inactivation and activation.  Conclusion  Stearic acid induced by high-fat diet can affect the function of L-type calcium channel on VSMCs membrane of mesenteric vessels.

     

    • FullText(HTML)
  • loading
    • References(20)
  • [1]
    CHAREONRUNGRUEANGCHAI K, WONGKAWINWOOT K, ANOTHAISINTAWEE T, et al. Dietary factors and risks of cardiovascular diseases: an umbrella review[J]. Nutrients, 2020, 12(4): 1088. doi: 10.3390/nu12041088
    [2]
    KOCHAN Z, SZUPRYCZYNSKA N, MALGORZEWICZ S, et al. Dietary lipids and dyslipidemia in chronic kidney disease[J]. Nutrients, 2021, 13(9): 3138. doi: 10.3390/nu13093138
    [3]
    刘尚红, 孙丽姣, 李娟, 等. 宁夏1985—2014年中小学生超重肥胖变化趋势及模型预测[J]. 中国学校卫生, 2018, 39(12): 1855-1857, 1860. https://www.cnki.com.cn/Article/CJFDTOTAL-XIWS201812028.htm

    LIU S H, SUN L J, LI J, et al. Secular trend and model prediction of overweight and obesity epidemiology among primary and middle school students in Ningxia[J]. Chinese Journal of School Health, 2018, 39(12): 1855-1857, 1860. https://www.cnki.com.cn/Article/CJFDTOTAL-XIWS201812028.htm
    [4]
    PEREIRA A R, OLIVEIRA A. Dietary interventions to prevent childhood obesity: a literature review[J]. Nutrients, 2021, 13(10): 3447. doi: 10.3390/nu13103447
    [5]
    TAYLOR L E, RAMIREZ L A, MUSALL J B, et al. Tipping the scales: are females more at risk for obesity- and high-fat diet-induced hypertension and vascular dysfunction?[J]. Br J Pharmacol, 2019, 176(21): 4226-4242. doi: 10.1111/bph.14783
    [6]
    NOH M R, KONG M J, HAN S J, et al. Isocitrate dehydrogenase 2 deficiency aggravates prolonged high-fat diet intake-induced hypertension[J]. Redox Biol, 2020, 34: 101548. DOI: 10.1016/j.redox.2020.101548.
    [7]
    PALLAZOLA V A, DAVIS D M, WHELTON S P, et al. A clinician ' s guide to healthy eating for cardiovascular disease prevention[J]. Mayo Clin Proc Innov Qual Outcomes, 2019, 3(3): 251-267. doi: 10.1016/j.mayocpiqo.2019.05.001
    [8]
    GOUAREF I, BOUAZZA A, ABDERRHMANE S A, et al. Lipid profile modulates cardiometabolic risk biomarkers including hypertension in people with type-2 diabetes: a focus on unbalanced ratio of plasma polyunsaturated/saturated fatty acids[J]. Molecules, 2020, 25(18): 4315. doi: 10.3390/molecules25184315
    [9]
    LI X, FENG X Q, LU L K, et al. Prenatal hypoxia plus postnatal high-fat diet exacerbated vascular dysfunction via up-regulated vascular Cav1.2 channels in offspring rats[J]. J Cell Mol Med, 2019, 23(2): 1183-1196. doi: 10.1111/jcmm.14020
    [10]
    刘冲, 云惠聪, 张秀兰, 等. 内蒙古自治区3~18岁儿童体格发育调查[J]. 中华全科医学, 2022, 20(2): 327-331. doi: 10.16766/j.cnki.issn.1674-4152.002344

    LIU C, YUN H C, ZHANG X L, et al. Survey of physical development in children aged 3 to 18 years in Inner Mongolia[J]. Chinese Journal of General Practice, 2022, 20(2): 327-331. doi: 10.16766/j.cnki.issn.1674-4152.002344
    [11]
    郝丽鑫, 张兵, 王惠君, 等. 1989—2018年我国15个省(自治区、直辖市)18~35岁成年人超重和肥胖变化趋势及流行特征[J]. 环境与职业医学, 2022, 39(5): 471-477. https://www.cnki.com.cn/Article/CJFDTOTAL-LDYX202205001.htm

    HAO L X, ZHANG B, WANG H J, et al. Trends and epidemic characteristics of overweight and obesity among adults aged 18-35 in 15 provinces (autonomous regions/municipalities) of China from 1989 to 2018[J]. Journal of Environmental and Occupational Medicine, 2022, 39(5): 471-477. https://www.cnki.com.cn/Article/CJFDTOTAL-LDYX202205001.htm
    [12]
    YUAN S, YU H J, LIU M W, et al. Fat intake and hypertension among adults in china: the modifying effects of fruit and vegetable intake[J]. Am J Prev Med, 2020, 58(2): 294-301. doi: 10.1016/j.amepre.2019.09.004
    [13]
    GASPAR R S, UNSWORTH A J, AL-DIBOUNI A, et al. Maternal and offspring high-fat diet leads to platelet hyperactivation in male mice offspring[J]. Sci Rep, 2021, 11(1): 1473. doi: 10.1038/s41598-020-80373-3
    [14]
    曾荣, 闫家富, 郑恪扬, 等. 空腹甘油三酯血糖指数与原发性高血压患者动脉硬化的相关性研究[J]. 中华全科医学, 2021, 19(8): 1262-1264, 1286. doi: 10.16766/j.cnki.issn.1674-4152.002035

    ZENG R, YAN J F, ZHENG K Y, et al. Relationship between fasting triglyceride glucose index and arteriosclerosis in patients with essential hypertension[J]. Chinese Journal of General Practice, 2021, 19(8): 1262-1264, 1286. doi: 10.16766/j.cnki.issn.1674-4152.002035
    [15]
    NOH J W, JUN M S, YANG H K, et al. Cellular and molecular mechanisms and effects of berberine on obesity-induced inflammation[J]. Biomedicines, 2022, 10(7): 1739. doi: 10.3390/biomedicines10071739
    [16]
    XU T, ZHAO M, LI H, et al. Antenatal dexamethasone exposure impairs the high-conductance Ca2+-Activated K+ channels via epigenetic alteration at gene promoter in male offspring[J]. Arterioscler Thromb Vasc Biol, 2020, 40(11): e284-e295.
    [17]
    XU T, JI B Y, LI L J, et al. Antenatal dexamethasone exposure impairs vascular contractile functions via upregulating IP3 Receptor 1 and Cav1.2 in adult male offspring[J]. Hypertension, 2022, 79(9): 1997-2007.
    [18]
    LI H, JI B Y, XU T, et al. Antenatal Hypoxia affects pulmonary artery contractile functions via downregulating L-type Ca2+ channels subunit Alpha1 C in adult male offspring[J]. J Am Heart Assoc, 2021, 10(8): e019922. DOI: 10.1161/JAHA.120.019922.
    [19]
    CHANG P Y, CHANG S F, CHANG T Y, et al. Synergistic effects of electronegative-LDL- and palmitic-acid-triggered IL-1β production in macrophages via LOX-1 and voltage-gated-potassium-channel-dependent pathways[J]. J Nutr Biochem, 2021, 97: 108767. DOI: 10.1016/j.jnutbio.2021.108767.
    [20]
    WANG Y, WANG X J, ZHAO L M, et al. Oxidative stress induced by palmitic acid modulates KCa2.3 channels in vascular endothelium[J]. Exp Cell Res, 2019, 383(2): 111552. DOI: 10.1016/j.yexcr.2019.111552.
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(1)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (279) PDF downloads(8) Cited by()
    Proportional views
    Related

    Address：No. 287, Changhuai Road, Bengbu, Anhui Province, 233004, P.R.ChinaphoneNo：0552-3066635； 0552-3051890Email：zhqkyx@163.com

    Copyright © Chinese Journal of General Practice皖ICP备2020018345号-2

    Supported by: Beijing Renhe Information Technology Co., Ltd.    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return