Volume 21 Issue 3
Mar.  2023
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Article Navigation >  Chinese Journal of General Practice  > 2023  >  21(3): 396-400
CAO Juan, LI Lingling, YAO Yao, XIAO Yunyun, WU Wenhui, LU Bangchao, DING Congzhu. Correlation between oxidative stress related cell senescence indicators and osteoarthritis[J]. Chinese Journal of General Practice, 2023, 21(3): 396-400. doi: 10.16766/j.cnki.issn.1674-4152.002892
Citation: CAO Juan, LI Lingling, YAO Yao, XIAO Yunyun, WU Wenhui, LU Bangchao, DING Congzhu. Correlation between oxidative stress related cell senescence indicators and osteoarthritis[J]. Chinese Journal of General Practice, 2023, 21(3): 396-400. doi: 10.16766/j.cnki.issn.1674-4152.002892
shu

Correlation between oxidative stress related cell senescence indicators and osteoarthritis

doi: 10.16766/j.cnki.issn.1674-4152.002892
  • 1.

    Department of Geriatrics, Drum Tower Hospital Affiliated Nanjing University Medical College, Nanjing, Jiangsu 210009, China

Funds:

 ZKX17020

 BJ19003

  • Received Date: 2022-07-02
    Available Online: 2023-04-19
    Abstract
  •   Objective  To investigate the correlation between chondrocyte senescence and osteoarthritis (OA) by detecting chondrocyte senescence related indicators.  Methods  The OA patients and non-OA patients hospitalized in the Department of Orthopedics in Nanjing Drum Tower Hospital were selected from January 2018 to December 2020. Normal knee chondrocytes and OA knee chondrocytes were isolated and cultured, and divided into normal human chondrocytes group (control group) and OA chondrocytes group (OA group). P2 generation cells were selected for the experiment. β galactosidase staining was performed. The reactive oxygen species (ROS) level and mitochondrial membrane potential were detected by flow cytometry. The mRNA expressions of P21 and P53 genes were detected by quantitative PCR (qPCR), and the protein levels of P21 and P53 in chondrocytes were detected by Western blotting.  Results  The positive rate of β-galactosidase staining in chondrocytes in the OA group was significantly higher than that in the control group. The level of ROS in the OA group [(24.72±2.40) %] was significantly higher than that in the control group [(4.96±0.14) %, P < 0.01]. The green fluorescence intensity of JC-1 staining in the OA group [(21.87±3.03) %] was significantly higher than that in the control group [(3.13±0.25) %, P < 0.01]. The mRNA expressions of P21 and P53 genes in the OA group were (2.72±0.21) and (2.90±0.23), which were significantly higher than those in the control group (1.00±0.03) and (1.00±0.06), all P < 0.05. The expression levels of cyclin P21 and P53 in the OA group [(0.51±0.02) and (0.55±0.03)] were also significantly higher than those in the control group [(0.40±0.03) and (0.45±0.02), P < 0.01].  Conclusion  Compared with normal chondrocytes, OA chondrocytes senesce significantly. Oxidative stress-induced cell senescence may play a key role in the pathogenesis of OA. Anti-oxidative stress may be an effective target for the treatment of OA.

     

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