Chlorogenic acid effects of intestinal epithelial barrier function with septic rats through TLR4/NF-κB signaling pathway

WANG Jinxin; RAO Chunhui; DAI Bo; YU Huiqin; WANG Zhigang

doi:10.16766/j.cnki.issn.1674-4152.002894

Volume 21 Issue 3

Mar. 2023

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Article Navigation > Chinese Journal of General Practice > 2023 > 21(3): 405-408

WANG Jinxin, RAO Chunhui, DAI Bo, YU Huiqin, WANG Zhigang. Chlorogenic acid effects of intestinal epithelial barrier function with septic rats through TLR4/NF-κB signaling pathway[J]. Chinese Journal of General Practice, 2023, 21(3): 405-408. doi: 10.16766/j.cnki.issn.1674-4152.002894

Citation:

WANG Jinxin, RAO Chunhui, DAI Bo, YU Huiqin, WANG Zhigang. Chlorogenic acid effects of intestinal epithelial barrier function with septic rats through TLR4/NF-κB signaling pathway[J]. Chinese Journal of General Practice, 2023, 21(3): 405-408. doi: 10.16766/j.cnki.issn.1674-4152.002894

Citation:

WANG Jinxin, RAO Chunhui, DAI Bo, YU Huiqin, WANG Zhigang. Chlorogenic acid effects of intestinal epithelial barrier function with septic rats through TLR4/NF-κB signaling pathway[J]. Chinese Journal of General Practice, 2023, 21(3): 405-408. doi: 10.16766/j.cnki.issn.1674-4152.002894

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Chlorogenic acid effects of intestinal epithelial barrier function with septic rats through TLR4/NF-κB signaling pathway

doi: 10.16766/j.cnki.issn.1674-4152.002894

1.
Department of Gastroenterology, Zhejiang Medical Health Group Hangzhou Hospital, Hangzhou, Zhejiang 310022, China

Funds:

2019KY522

Received Date: 2022-04-20
Available Online: 2023-04-19

Abstract

Abstract

Objective To observe the effect of chlorogenic acid on reducing intestinal oxidative stress injury and improving intestinal cell barrier function through TLR4/NF-κB signaling pathway in septic rats. Methods Sixty SD rats were randomly divided into four groups by the random number table method: sham operation group, model control group and chlorogenic acid (low and high dose) group, with 15 rats in each group. 24 hours after operation, the levels of tumor necrosis factor-α (TNF-α), interleukin (IL-6) and IL-10 were detected by enzyme-linked immunosorbent assay (ELISA), the levels of nitric oxide (NO), superoxide dismutase (SOD) and malondialdehyde (MDA) in small intestine were measured by colorimetry, and western blot was used to detect claudin-1, ZO-1 and TLR-4/NF-κB pathway related protein expression. Results Compared with the model control group, Chiu score in low dose group (4.17±0.22) and high dose group (1.95±0.08) decreased significantly (t=4.159, 60.048, all P < 0.01). The levels of TNF-α, IL-6, IL-10 and the contents of NO and MDA in the small intestine in high dose group were significantly decreased (all P < 0.05), the content of SOD, the expression of Claudin-1, ZO-1 protein, MyD88, TLR-4 protein and p-NF-κB p65 (Ser536) increased significantly (all P < 0.05). Conclusion Chlorogenic acid can reduce serum inflammatory response, reduce oxidative damage of small intestine in septic rats through inhibiting TLR4/NF-κB signal transduction, and then improve intestinal barrier function.
- Chlorogenic acid,
- TLR4/NF-κB signal pathway,
- Intestinal flora,
- Intestinal epithelial barrier function

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References

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Chlorogenic acid effects of intestinal epithelial barrier function with septic rats through TLR4/NF-κB signaling pathway

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