Volume 21 Issue 4
Apr.  2023
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Article Navigation >  Chinese Journal of General Practice  > 2023  >  21(4): 544-548
HE Xuxu, ZHAO Meng, YANG Yiqun, ZUO Lugen, GENG Zhijun, GE Sitang, CHEN Deli. UGT8 expression in gastric cancer tissues and its regulatory effect on gastric cancer metastasis[J]. Chinese Journal of General Practice, 2023, 21(4): 544-548. doi: 10.16766/j.cnki.issn.1674-4152.002927
Citation: HE Xuxu, ZHAO Meng, YANG Yiqun, ZUO Lugen, GENG Zhijun, GE Sitang, CHEN Deli. UGT8 expression in gastric cancer tissues and its regulatory effect on gastric cancer metastasis[J]. Chinese Journal of General Practice, 2023, 21(4): 544-548. doi: 10.16766/j.cnki.issn.1674-4152.002927
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UGT8 expression in gastric cancer tissues and its regulatory effect on gastric cancer metastasis

doi: 10.16766/j.cnki.issn.1674-4152.002927
  • 1.

    Graduate School of Bengbu Medical College, Bengbu, Anhui 233030, China

Funds:

 KJ2021A0685

  • Received Date: 2022-09-05
    Available Online: 2023-05-31
    Abstract
  •   Objective  To study the expression of uridine diphosphate-galactose glycosyltransferase 8(UGT8) in gastric cancer and its association with long-term prognosis and its effect on the metastatic ability of gastric cancer.  Methods  A total of 109 patients who underwent radical gastric cancer resection in the First Affiliated Hospital of Bengbu Medical College from December 2013 to October 2016 were included in this study.The expression level of UGT8 in gastric cancer tissues was detected by immunohistochemistry and western blotting.Statistical methods were applied to analyze the relationship between UGT8 level and clinicopathological parameters in gastric cancer.The Cox regression and ROC curve were used to evaluate the independent risk factors affecting the 5-year survival rate and the diagnostic efficacy of UGT8.The GEO database was used to obtain a dataset of gastric cancer, and GO and KEGG enrichment analysis was performed on UGT8.The effect of UGT8 on metastasis of gastric cancer cells (MGC-803) was analyzed in vitro.  Results  The expression level of UGT8 in gastric cancer tissues was significantly higher than that in adjacent tissues (P < 0.01);The proportion of patients with increased tumor diameter (≥5 cm), T3-4 stage, N2-3 stage, serum CEA≥5 μg/L and serum CA19-9≥37 kU/L in the UGT8 high expression group was significantly higher than that in the UGT8 low expression group (all P < 0.05).Cox regression analysis showed that high UGT8 expression was an independent risk factor affecting the 5-year survival rate of gastric cancer patients.ROC curve analysis showed that UGT8 had a sensitivity of 73.00% and a specificity of 78.30% for predicting death at 5 years after surgery in patients with gastric cancer (P < 0.01).GO enrichment analysis showed that UGT8 was involved in cadherin binding activity, and KEGG enrichment results showed that UGT8 was mainly involved in adhesion pathways.Western blotting showed that UGT8 promoted the phosphorylation level of SDF-1 and CXCR4 in metastasis-related signals (P < 0.05).  Conclusion  The high expression of UGT8 in gastric cancer tissues is associated with poor prognosis, and may be involved in the metastasis of gastric cancer cells by regulating SDF-1/CXCR4 signaling pathway.

     

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