Volume 21 Issue 6
Jun.  2023
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Article Navigation >  Chinese Journal of General Practice  > 2023  >  21(6): 962-965
CHU Jicheng, HE Yu, YE Guoliu, WANG Liqun. Significance of HPV-E6/E7 mRNA examination in stratification management of non 16 and 18 high risk HPV infection patients[J]. Chinese Journal of General Practice, 2023, 21(6): 962-965. doi: 10.16766/j.cnki.issn.1674-4152.003025
Citation: CHU Jicheng, HE Yu, YE Guoliu, WANG Liqun. Significance of HPV-E6/E7 mRNA examination in stratification management of non 16 and 18 high risk HPV infection patients[J]. Chinese Journal of General Practice, 2023, 21(6): 962-965. doi: 10.16766/j.cnki.issn.1674-4152.003025
shu

Significance of HPV-E6/E7 mRNA examination in stratification management of non 16 and 18 high risk HPV infection patients

doi: 10.16766/j.cnki.issn.1674-4152.003025

  • Department of Obstetrics and Gynecology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

Funds:

 KJ2019A0343

 BYKY2019119ZD

  • Received Date: 2022-08-16
    Available Online: 2023-08-26
    Abstract
  •   Objective  To evaluate the application value of the human papilloma virus (HPV)-E6/E7mRNA examination in stratification management of patients with non-16 and 18 high-risk HPV infection and atypical squamous cell of negative for intraepithelial lesion or malign (NILM).  Methods  The clinical data of 383 patients with HPV-E6/E7 mRNA detection and colposcopy biopsy who were not at high risk of HPV infection type 16 and 18 and had negative cervical TCT at the First Affiliated Hospital of Bengbu Medical College from October 2020 to October 2021 were analyzed. The expression levels of HPV-E6/E7 mRNA were analyzed in different levels of cervical lesions.  Results  The 383 patients were divided into 252 cases of chronic cervical inflammation, 98 cases of low-grade squamous intraepithelial lesion (LSIL), and 33 cases of high-grade squamous intraepithelial lesion and above (HSIL+). The positive rate of HPV-E6/E7 mRNA were 40.5%, 64.3% and 66.7%, respectively (P < 0.001). There was a positive correlation between the pathological grade of cervical lesions and the expression of HPV-E6/E7 mRNA (r=0.254, P < 0.05). The AUC of HPV-E6/E7 mRNA examination was 0.623 and cut-off was 741.092 copies/mL. When cut-off was used as criteria for HSIL and above lesions, the sensitivity and specificity of HPV-E6/E7 mRNA were 63.6% and 64.3%, respectively, and the Youden index was 0.279.  Conclusion  HPV-E6/E7 mRNA have an important value for stratification management in patients with high-risk HPV non-16 and 18 infection and negative cervical TCT, which is hopeful to reduce the colposcopy referral rate.

     

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    • References(20)
  • [1]
    马梅, 晋茂生, 叶国柳. HPV分型、SCC-Ag与NLR联合对早期宫颈癌淋巴结转移的预测作用[J]. 中华全科医学, 2022, 20(4): 578-582. doi: 10.16766/j.cnki.issn.1674-4152.002404

    MA M, JIN M S, YE G L. Predictive effect of HPV typing, SCC-Ag and NLR on lymph node metastasis of early cervical cancer[J]. Chinese Journal of General Practice, 2022, 20(4): 578-582. doi: 10.16766/j.cnki.issn.1674-4152.002404
    [2]
    OLA F, MIRIAM E K, HELENA L, et al. HPV-mRNA and HPV-DNA detection in samples taken up to seven years before severe dysplasia of cervix uteri[J]. Int J Cancer, 2019, 144(5): 1073-1081. doi: 10.1002/ijc.31819
    [3]
    黄珊珊, 徐凤娟, 成雁, 等. 高危型HPV E6/E7 mRNA与HC2 HPV-DNA联合检测在宫颈病变筛查中价值[J]. 中华实用诊断与治疗杂志, 2020, 34(6): 577-580. https://www.cnki.com.cn/Article/CJFDTOTAL-HNZD202006010.htm

    HUANG S S, XU F J, CHENG Y, et al. Joint detection of high-risk HPV E6/E7 mRNA and HC2 HPV-DNA in cervical lesions screening[J]. Journal of Chinese Practical Diagnosis and Therapy, 2020, 34(6): 577-580. https://www.cnki.com.cn/Article/CJFDTOTAL-HNZD202006010.htm
    [4]
    SASLOW D, SOLOMON D, LAWSON H W, et al. American Cancer Society, American Society for Colposcopy and Cervical Pathology, and American Society for Clinical Pathology screening guidelines for the prevention and early detection of cervical cancer[J]. CA Cancer J Clin, 2012, 62(3): 147-172. doi: 10.3322/caac.21139
    [5]
    DERBIE A, MEKONNEN D, WOLDEAMANUEL Y, et al. HPV E6/E7 mRNA test for the detection of high grade cervical intraepithelial neoplasia (CIN2+): a systematic review[J]. Infect Agents Cancer, 2020, 15(1): 9. DERBIE A, MEKONNEN D, WOLDEAMANUEL Y, et al. HPV E6/E7 mRNA test for the detection of high grade cervical intraepithelial neoplasia (CIN2+): a systematic review[J]. Infect Agents Cancer, 2020, 15(1): 9.
    [6]
    闫海洋, 闵少菊, 沈艳, 等. HPV E6/E7 mRNA检测在HPV分型及宫颈疾病筛查中的作用研究[J]. 中国实用妇科与产科杂志, 2018, 34(8): 923-927. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGSF201808027.htm

    YAN H Y, MIN S J, SHEN Y, et al. Clinical study of HPV E6/E7 mRNA detection in HPV typing and screening of cervical diseases[J]. Chinese Journal of Practical Gynecology and Obstetrics, 2018, 34(8): 923-927. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGSF201808027.htm
    [7]
    单继烈, 叶素梅, 王兰英, 等. 液基薄层细胞学检测、高危型人乳头状瘤病毒-DNA和人乳头状瘤病毒E6/E7 mRNA检测在宫颈癌早期筛查中的临床价值[J]. 中国卫生检验杂志, 2018, 28(6): 667-669. https://www.cnki.com.cn/Article/CJFDTOTAL-ZWJZ201806008.htm

    SHAN J L, YE S M, WANG L Y, et al. Clinical value of TCT, high-risk HPV-DNA and HPV E6/E7 mRNA detection in early screening of cervical cancer[J]. Chinese Journal of Health Laboratory Technology, 2018, 28(6): 667-669. https://www.cnki.com.cn/Article/CJFDTOTAL-ZWJZ201806008.htm
    [8]
    FONTHAM E T H, WOLF A M D, CHURCH T R, et al. Cervical cancer screening for individuals at average risk: 2020 guideline update from the American Cancer Society[J]. CA Cancer J Clin, 2020, 70(5): 321-346. doi: 10.3322/caac.21628
    [9]
    EGEMEN D, CHEUNG L C, CHEN X, et al. 2019 ASCCP risk-based management consensus guidelines: methods for risk estimation, recommended management, and validation[J]. Low Genit Tract Dis, 2020, 24(2): 90-101. doi: 10.1097/LGT.0000000000000528
    [10]
    NAH E H, CHO S, KIM S, et al. Human papillomavirus genotype distribution among 18, 815 women in 13 Korean cities and relationship with cervical cytology findings[J]. Ann Lab Med, 2017, 37(5): 426-433. doi: 10.3343/alm.2017.37.5.426
    [11]
    JING L, ZHONG X, HUANG W, et al. HPV genotypes and associated cervical cytological abnormalities in women from the Pearl River Delta region of Guangdong province, China: a cross-sectional study[J]. BMC Infect Dis, 2014, 14: 388. doi: 10.1186/1471-2334-14-388
    [12]
    WANG L, WU B, LI J, et al. Prevalence of human papillomavirus and its genotype among 1336 invasive cervical cancer patients in hunan province, central south China[J]. J Med Virol, 2015, 87(3): 516-521. doi: 10.1002/jmv.24094
    [13]
    张靖, 高波, 康赟, 等. 中国女性宫颈人乳头瘤病毒感染型别分布区域性特征的Meta分析[J]. 中华微生物学和免疫学杂志, 2014, 34(12): 913-920. doi: 10.3760/cma.j.issn.0254-5101.2014.12.008

    ZHANG J, GAO B, KANG Y, et al. Genotype distribution of human papillomavirus strains in cervix samples from Chinese women: a meta-analysis[J]. Chinese Journal of Microbiology and Immunology, 2014, 34(12): 913-920. doi: 10.3760/cma.j.issn.0254-5101.2014.12.008
    [14]
    王丽, 何玉, 许驰. HPV分型检测在宫颈病变诊断中的价值[J]. 中国妇幼保健, 2021, 36(6): 1232-1234. https://www.cnki.com.cn/Article/CJFDTOTAL-ZFYB202106005.htm

    WANG L, HE Y, XU C. The value of HPV typing in the diagnosis of cervical lesions[J]. Maternal and Child Health Care of China, 2021, 36(6): 1232-1234. https://www.cnki.com.cn/Article/CJFDTOTAL-ZFYB202106005.htm
    [15]
    WRIGHT T C, STOLER M H, PARVU V, et al. Risk detection for high-grade cervical disease using onclarity HPV extended genotyping in women, ≥21 years of age, with ASC-US or LSIL cytology[J]. Gynecol Oncol, 2019, 154(2): 360-367. doi: 10.1016/j.ygyno.2019.05.012
    [16]
    DEMARCO M, HYUN N, CARTER-POKRAS O, et al. A study of type-specific HPV natural history and implications for contemporary cervical cancer screening programs[J]. Clin Med, 2020, 22: 100293. DOI: 10.1016/j.eclinm.2020.100293.
    [17]
    BONDE J H, SANDRI M T, GARY D S, et al. Clinical utility of human papillomavirus genotyping in cervical cancer screening: a systematic review[J]. J Low Genit Tract Di, 2019, 24(1): 1-13.
    [18]
    CHEN L H, DONG B H, ZHANG Q Y, et al. HR-HPV viral load quality detection provide more accurate prediction for residual lesions after treatment: a prospective cohort study in patients with high-grade squamous lesions or worse[J]. Med Oncol, 2020, 37(5): 37. doi: 10.1007/s12032-020-01363-z
    [19]
    倪昌雪, 王艳, 叶国柳, 等. HPV E6/E7 mRNA检查对宫颈细胞学ASCUS的分流价值[J]. 中华全科医学, 2021, 19(2): 198-201, 255. doi: 10.16766/j.cnki.issn.1674-4152.001766

    NI C X, WANG Y, YE G L, et al. The shunt value of HPV E6/E7mRNA examination in cervical cytology ASCUS[J]. Chinese Journal of General Practice, 2021, 19(2): 198-201, 255. doi: 10.16766/j.cnki.issn.1674-4152.001766
    [20]
    COQUILLARD G B, PALAO B K. Patterson, quantification of intracellular HPV E6/E7 mRNA expression increases the specificity and positive predictive value of cervical cancer screening compared to HPV DNA[J]. Gynecol Oncol, 2011, 120(1): 89-93. doi: 10.1016/j.ygyno.2010.09.013
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