Volume 21 Issue 7
Jul.  2023
Turn off MathJax
Article Contents
Article Navigation >  Chinese Journal of General Practice  > 2023  >  21(7): 1101-1104
LI Xiaoxiong, LI Jinping, HUANG Jie, MA Jingjing, HOU Mingliang, MA Linqiu, WANG Congguo, ZHOU Huadong. Analysis of circRNA and mRNA expression profiles in Alzheimer's disease mice treated with islet amyloid polypeptide[J]. Chinese Journal of General Practice, 2023, 21(7): 1101-1104. doi: 10.16766/j.cnki.issn.1674-4152.003059
Citation: LI Xiaoxiong, LI Jinping, HUANG Jie, MA Jingjing, HOU Mingliang, MA Linqiu, WANG Congguo, ZHOU Huadong. Analysis of circRNA and mRNA expression profiles in Alzheimer's disease mice treated with islet amyloid polypeptide[J]. Chinese Journal of General Practice, 2023, 21(7): 1101-1104. doi: 10.16766/j.cnki.issn.1674-4152.003059
shu

Analysis of circRNA and mRNA expression profiles in Alzheimer's disease mice treated with islet amyloid polypeptide

doi: 10.16766/j.cnki.issn.1674-4152.003059
  • 1.

    Departments of Neurology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

Funds:

 81771177

  • Received Date: 2022-09-22
    Available Online: 2023-08-28
    Abstract
  •   Objective  To investigate the changes of circulating RNA (circRNA) and messenger RNA (mRNA) expression profiles in brain tissue of mice with Alzheimer's disease (AD) treated with islet amyloid polypeptide (IAPP) and the molecular mechanisms.  Methods  According to the number table method, 6 APP/PS1 mice were randomly divided into IAPP intervention group and reference group, 3 mice in each group. The intervention group mice were injected intraperitoneally with 0.5 μmol/L of IAPP (200 μg/kg), the reference group mice were injected with the same dose of phosphate buffer saline. Differentially expressed circRNAs and mRNAs between the two groups of mice were detected using gene chip technology. Chip results were validated by qRT-PCR. Differentially expressed mRNAs were analyzed by GO and KEGG pathways.  Results  According to the screening criteria of difference fold change (|FC|) ≥1.5 and P < 0.05, there were 899 differentially expressed circRNAs, 343 up-regulated and 556 down-regulated, and 2 257 differentially expressed mRNAs, 1 310 up-regulated and 947 down-regulated, between the IAPP intervention group and the reference group. qRT-PCR validation results were consistent with the gene chip results. GO analysis showed that the differentially expressed mRNAs were mainly enriched in G protein-coupled receptor, cell metabolism and other biological functions. KEGG pathway analysis showed that they were mainly involved in citrate cycle, proteasome, IL-17 and chemokine signaling pathway, etc.  Conclusion  The expression profiles of circRNAs and mRNAs in the brain tissue of AD mice were significantly changed after intraperitoneal injection of IAPP, suggesting that these differentially expressed circRNAs and mRNAs may be the key mechanisms of IAPP treatment in AD mice.

     

    • FullText(HTML)
  • loading
    • References(21)
  • [1]
    王琼, 王国平. 阿尔茨海默病的诊断与治疗[J]. 中华全科医学, 2019, 17(8): 1255-1256. http://www.zhqkyx.net/cn/article/id/18f73bba-8d0a-4fc0-8880-f792d4b6bd77

    WANG Q, WANG G P. Diagnosis and treatment of Alzheimer's disease[J]. Chinese Journal of General Practice, 2019, 17(8): 1255-1256. http://www.zhqkyx.net/cn/article/id/18f73bba-8d0a-4fc0-8880-f792d4b6bd77
    [2]
    邱聪龙, 张怡, 陈寿林, 等. 不同程度阿尔茨海默病患者血脂水平的研究[J]. 中华全科医学, 2020, 18(12): 2043-2046. doi: 10.16766/j.cnki.issn.1674-4152.001684

    QIU C L, ZHANG Y, CHEN S L, et al. A study of serum lipid levels in patients with different degrees of Alzheimer's disease[J]. Chinese Journal of General Practice, 2020, 18(12): 2043-2046. doi: 10.16766/j.cnki.issn.1674-4152.001684
    [3]
    MARMENTINI C, BRANCO R C S, BOSCHERO A C, et al. Islet amyloid toxicity: from genesis to counteracting mechanisms[J]. J Cell Physiol, 2022, 237(2): 1119-1142. doi: 10.1002/jcp.30600
    [4]
    BHARADWAJ P, SOLOMON T, SAHOO B R, et al. Amylin and beta amyloid proteins interact to form amorphous heterocomplexes with enhanced toxicity in neuronal cells[J]. Sci Rep, 2020, 10(1): 10356. DOI: 10.1038/s41598-020-66602-9.
    [5]
    DHARMARAJ G L, ARIGO F D, YOUNG K A, et al. Novel amylin analogues reduce amyloid-β cross-seeding aggregation and neurotoxicity[J]. J Alzheimers Dis, 2022, 87(1): 373-390. doi: 10.3233/JAD-215339
    [6]
    SINGH S, YANG F, SIVILS A, et al. Amylin and secretases in the pathology and treatment of Alzheimer's disease[J]. Biomolecules, 2022, 12(7): 996. doi: 10.3390/biom12070996
    [7]
    GAN Q N, YAO H B, NA H, et al. Effects of amylin against amyloid-β-induced tauopathy and synapse loss in primary neurons[J]. J Alzheimers Dis, 2019, 70(4): 1025-1040. doi: 10.3233/JAD-190161
    [8]
    HUANG A Q, ZHENG H X, WU Z Y, et al. Circular RNA-protein interactions: functions, mechanisms, and identification[J]. Theranostics, 2020, 10(8): 3503-3517. doi: 10.7150/thno.42174
    [9]
    COCHRAN K R, VEERARAGHAVAN K, KUNDU G, et al. Systematic identification of circRNAs in Alzheimer's disease[J]. Genes(Basel), 2021, 12(8): 1258.
    [10]
    LIN Z J, TANG X Z, WAN J, et al. Functions and mechanisms of circular RNAs in regulating stem cell differentiation[J]. RNA Biol, 2021, 18(12): 2136-2149. doi: 10.1080/15476286.2021.1913551
    [11]
    SOUDY R, KIMURA R, PATEL A, et al. Short amylin receptor antagonist peptides improve memory deficits in Alzheimer's disease mouse model[J]. Sci Rep, 2019, 9(1): 10942. DOI: 10.1038/s41598-019-47255-9.
    [12]
    PATRICK S, CORRIGAN R, GRIZZANTI J, et al. Neuroprotective effects of the amylin analog, pramlintide, on Alzheimer's disease are associated with oxidative stress regulation mechanisms[J]. J Alzheimers Dis, 2019, 69(1): 157-168. doi: 10.3233/JAD-180421
    [13]
    JEONG J K, DOW S A, YOUNG C N. Sensory circumventricular organs, neuroendocrine control, and metabolic regulation[J]. Metabolites, 2021, 11(8): 494. doi: 10.3390/metabo11080494
    [14]
    SERVIZI S, CORRIGAN R R, CASADESUS G. The importance of understanding amylin signaling mechanisms for therapeutic development in the treatment of Alzheimer's disease[J]. Curr Pharm Des, 2020, 26(12): 1345-1355. doi: 10.2174/1381612826666200318151146
    [15]
    MA N N, TIE C R, YU B, et al. Circular RNAs regulate its parental genes transcription in the AD mouse model using two methods of library construction[J]. FASEB J, 2020, 34(8): 10342-10356. doi: 10.1096/fj.201903157R
    [16]
    WU K, NIE B, LI L Y, et al. Bioinformatics analysis of high frequency mutations in myelodysplastic syndrome-related patients[J]. Ann Transl Med, 2021, 9(19): 1491. doi: 10.21037/atm-21-4094
    [17]
    JO S L, YANG H, LEE S R, et al. Curcumae radix decreases neurodegenerative markers through glycolysis decrease and TCA cycle activation[J]. Nutrients, 2022, 14(8): 1587. doi: 10.3390/nu14081587
    [18]
    KRISHNA-K K, BABY N, RAGHURAMAN R, et al. Regulation of aberrant proteasome activity re-establishes plasticity and long-term memory in an animal model of Alzheimer's disease[J]. FASEB J, 2020, 34(7): 9466-9479. doi: 10.1096/fj.201902844RR
    [19]
    CHOCRON E S, MUNKÁCSY E, KIM H S, et al. Genetic and pharmacologic proteasome augmentation ameliorates Alzheimer's-like pathology in mouse and fly APP overexpression models[J]. Sci Adv, 2022, 8(23): eabk2252. DOI: 10.1126/sciadv.abk2252.
    [20]
    CRISTIANO C, VOLPICELLI F, LIPPIELLO P, et al. Neutralization of IL-17 rescues amyloid-β-induced neuroinflammation and memory impairment[J]. Br J Pharmacol, 2019, 176(18): 3544-3557. doi: 10.1111/bph.14586
    [21]
    PARK J C, HAN S H, MOOK-JUNG I. Peripheral inflammatory biomarkers in Alzheimer's disease: a brief review[J]. BMB Rep, 2020, 53(1): 10-19. doi: 10.5483/BMBRep.2020.53.1.309
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(1)  / Tables(4)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (170) PDF downloads(5) Cited by()
    Proportional views
    Related

    Address：No. 287, Changhuai Road, Bengbu, Anhui Province, 233004, P.R.ChinaphoneNo：0552-3066635； 0552-3051890Email：zhqkyx@163.com

    Copyright © Chinese Journal of General Practice皖ICP备2020018345号-2

    Supported by: Beijing Renhe Information Technology Co., Ltd.    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return