Volume 21 Issue 7
Jul.  2023
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Article Navigation >  Chinese Journal of General Practice  > 2023  >  21(7): 1101-1104
LI Xiaoxiong, LI Jinping, HUANG Jie, MA Jingjing, HOU Mingliang, MA Linqiu, WANG Congguo, ZHOU Huadong. Analysis of circRNA and mRNA expression profiles in Alzheimer's disease mice treated with islet amyloid polypeptide[J]. Chinese Journal of General Practice, 2023, 21(7): 1101-1104. doi: 10.16766/j.cnki.issn.1674-4152.003059
Citation: LI Xiaoxiong, LI Jinping, HUANG Jie, MA Jingjing, HOU Mingliang, MA Linqiu, WANG Congguo, ZHOU Huadong. Analysis of circRNA and mRNA expression profiles in Alzheimer's disease mice treated with islet amyloid polypeptide[J]. Chinese Journal of General Practice, 2023, 21(7): 1101-1104. doi: 10.16766/j.cnki.issn.1674-4152.003059
shu

Analysis of circRNA and mRNA expression profiles in Alzheimer's disease mice treated with islet amyloid polypeptide

doi: 10.16766/j.cnki.issn.1674-4152.003059
  • 1.

    Departments of Neurology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

Funds:

 81771177

  • Received Date: 2022-09-22
    Available Online: 2023-08-28
    Abstract
  •   Objective  To investigate the changes of circulating RNA (circRNA) and messenger RNA (mRNA) expression profiles in brain tissue of mice with Alzheimer's disease (AD) treated with islet amyloid polypeptide (IAPP) and the molecular mechanisms.  Methods  According to the number table method, 6 APP/PS1 mice were randomly divided into IAPP intervention group and reference group, 3 mice in each group. The intervention group mice were injected intraperitoneally with 0.5 μmol/L of IAPP (200 μg/kg), the reference group mice were injected with the same dose of phosphate buffer saline. Differentially expressed circRNAs and mRNAs between the two groups of mice were detected using gene chip technology. Chip results were validated by qRT-PCR. Differentially expressed mRNAs were analyzed by GO and KEGG pathways.  Results  According to the screening criteria of difference fold change (|FC|) ≥1.5 and P < 0.05, there were 899 differentially expressed circRNAs, 343 up-regulated and 556 down-regulated, and 2 257 differentially expressed mRNAs, 1 310 up-regulated and 947 down-regulated, between the IAPP intervention group and the reference group. qRT-PCR validation results were consistent with the gene chip results. GO analysis showed that the differentially expressed mRNAs were mainly enriched in G protein-coupled receptor, cell metabolism and other biological functions. KEGG pathway analysis showed that they were mainly involved in citrate cycle, proteasome, IL-17 and chemokine signaling pathway, etc.  Conclusion  The expression profiles of circRNAs and mRNAs in the brain tissue of AD mice were significantly changed after intraperitoneal injection of IAPP, suggesting that these differentially expressed circRNAs and mRNAs may be the key mechanisms of IAPP treatment in AD mice.

     

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