Volume 21 Issue 7
Jul.  2023
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Article Navigation >  Chinese Journal of General Practice  > 2023  >  21(7): 1117-1120
ZHANG Yongqi, ZOU Zhenhai, WU Mengqi, LIU Beibei, GUO Yuanyuan, LIU Jianmin. Knockdown of EPHA2 regulates autophagy and biological behavior of bladder cancer cell through mTOR phosphorylation[J]. Chinese Journal of General Practice, 2023, 21(7): 1117-1120. doi: 10.16766/j.cnki.issn.1674-4152.003063
Citation: ZHANG Yongqi, ZOU Zhenhai, WU Mengqi, LIU Beibei, GUO Yuanyuan, LIU Jianmin. Knockdown of EPHA2 regulates autophagy and biological behavior of bladder cancer cell through mTOR phosphorylation[J]. Chinese Journal of General Practice, 2023, 21(7): 1117-1120. doi: 10.16766/j.cnki.issn.1674-4152.003063
shu

Knockdown of EPHA2 regulates autophagy and biological behavior of bladder cancer cell through mTOR phosphorylation

doi: 10.16766/j.cnki.issn.1674-4152.003063

  • Department of Urology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

Funds:

 AHWJ2021-a007

 Byycx21081

  • Received Date: 2022-11-09
    Available Online: 2023-08-28
    Abstract
  •   Objective  To explore the molecular mechanism by which Si-EPHA2 interferes with the biological behaviour of bladder cancer cells by targeting mTOR autophagy pathways.  Methods  The expression levels of EPHA2 in bladder cancer tissues and paracancerous tissues, as well as bladder cancer cells (T24) and normal urinary tract epithelial cells (SV-HUC-1) were examined by Western blotting assay and qRT-PCR assay. The expression of EPHA2 was knocked down in bladder cancer T24 cells by siRNA transfection to constitute an experimental model of bladder cancer cells with inhibited EPHA2 expression, and the cells were divided into si-NC blank for control group, and si-EPHA2 for experimental group. Changes in bladder cancer cell proliferation were detected by CCK 8 assay, changes in bladder cancer cell migration and invasion by Transwell assay, and changes in bladder cancer cell apoptosis by flow cytometry assay; the level of bladder cancer mTOR phosphorylation level and the expression of autophagy marker LC3 were detected by Western blotting assay after knockdown of EPHA2 expression; Knockdown of TSC1 was continued on top of knockdown of EPHA2-expressing T24 cells to detect changes in bladder cancer cell biology.  Results  EPHA2 showed higher expression levels in bladder cancer tissues and cells (P < 0.05); knockdown of EPHA2 decreased mTOR phosphorylation levels and increased autophagy levels (P < 0.05); knockdown of EPHA2 significantly inhibited the proliferation, migration and invasion ability of bladder cancer cells (P < 0.05) and promoted apoptosis (P < 0.05); on the basis of knockdown of EPHA2 expression, knockdown of TSC1 to promote mTOR phosphorylation partially reversed the effect of Si-EPHA2 on the biological behavior of bladder cancer cells (P < 0.05).  Conclusion  Knockdown of EPHA2 can target and inhibit mTOR phosphorylation in bladder cancer cells, enhance autophagy, inhibit the proliferation, migration and invasion ability of bladder cancer cell.

     

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