Volume 21 Issue 7
Jul.  2023
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Article Navigation >  Chinese Journal of General Practice  > 2023  >  21(7): 1143-1146
DU Dan, YANG Fan, HE Ping, CHEN Hui, ZHAO Yan, WANG Jing, LIU Haipeng, PENG Shuming. Changes of peripheral blood miR-145 level and its effect on PAI-1 in patients with repeated implantation failure[J]. Chinese Journal of General Practice, 2023, 21(7): 1143-1146. doi: 10.16766/j.cnki.issn.1674-4152.003069
Citation: DU Dan, YANG Fan, HE Ping, CHEN Hui, ZHAO Yan, WANG Jing, LIU Haipeng, PENG Shuming. Changes of peripheral blood miR-145 level and its effect on PAI-1 in patients with repeated implantation failure[J]. Chinese Journal of General Practice, 2023, 21(7): 1143-1146. doi: 10.16766/j.cnki.issn.1674-4152.003069
shu

Changes of peripheral blood miR-145 level and its effect on PAI-1 in patients with repeated implantation failure

doi: 10.16766/j.cnki.issn.1674-4152.003069

  • Reproductive Medicine Center, the First People's Hospital of chenzhou, Chenzhou, Hunan 423000, China

Funds:

 202205033251

 2020YYGL07

  • Received Date: 2023-03-06
    Available Online: 2023-08-28
    Abstract
  •   Objective  To investigate the changes of serum miR-145 levels and their impact on plasminogen activator inhibitor 1 (PAI-1) in patients with repeated implantation failure before egg retrieval and embryo transfer.  Methods  A total of 71 cases of repeated implantation failure (RIF) in the Reproductive Medicine Center of the First People's Hospital of chenzhou were included in the observation group, and 551 cases of successful pregnancy were included in the control group. The correlation between miR-145 and PAI-1, the independent relationship between miR-145 and RIF, and the evaluation value of miR-145 on RIF were analyzed.  Results  The levels of miR-145 [(1.09±0.13)ng/mL vs. (0.98±0.14) ng/mL, F=8.419, P=0.004; (1.19±0.16) ng/mL vs. (1.01±0.18) ng/mL, F=58.184, P<0.001] and PAI-1 [(21.36±6.28) ng/mL vs. (15.09±7.16) ng/mL, F=6.463, P=0.017; (25.36±6.18) ng/mL vs.(17.32±6.99) ng/mL, F=78.536, P<0.001] in the observation group were higher than those in the control group before egg retrieval and embryo transfer. There was a positive correlation between miR-145 and PAI-1 in the observation group before egg retrieval and embryo transfer (r=0.725, P<0.001; r=0.671, P<0.001). miR-145 was independently correlated with RIF before egg retrieval and embryo transfer (P<0.05). The AUC values of RIF evaluated by miR-145 before egg retrieval and embryo transfer were 0.690 and 0.774, respectively.  Conclusion  Patients with RIF infertility have abnormally high expression of miR-145, and it may reduce endometrial receptivity by targeting PAI-1 expression, thus participating in the process of RIF.

     

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