Significance of excluding anti-screening false negative in blood transfusion patients

YAN Ye

doi:10.16766/j.cnki.issn.1674-4152.003212

Volume 21 Issue 10

Oct. 2023

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Article Navigation > Chinese Journal of General Practice > 2023 > 21(10): 1732-1735

YAN Ye. Significance of excluding anti-screening false negative in blood transfusion patients[J]. Chinese Journal of General Practice, 2023, 21(10): 1732-1735. doi: 10.16766/j.cnki.issn.1674-4152.003212

Citation:

YAN Ye. Significance of excluding anti-screening false negative in blood transfusion patients[J]. Chinese Journal of General Practice, 2023, 21(10): 1732-1735. doi: 10.16766/j.cnki.issn.1674-4152.003212

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Significance of excluding anti-screening false negative in blood transfusion patients

doi: 10.16766/j.cnki.issn.1674-4152.003212

YAN Ye

Laboratory Medicine Center, Zhengzhou Yihe Hospital, Zhengzhou, Henan 450018, China

Funds:

2018020701

Received Date: 2022-07-20
Available Online: 2023-11-23

Abstract

Abstract

Objective To investigate the importance of excluding false negative antibody screening in patients to reduce the incidence of adverse transfusion reactions. Methods Screening and identification of irregular antibodies in 1 200 patients who received blood transfusion form January 2019 to June 2021 in Zhengzhou Yihe Hospital; the specific antibody plasma was diluted with AB type plasma, and the corresponding homozygous antigens and heterozygous antigens in the spectrum cells were reacted with the diluted plasma in saline, polybrene, anti-human globulin test card and papain medium, respectively. The highest dilution of plasma with a negative result of heterozygous antigen reaction is used to evaluate the agglutination intensity of the homozygous antigen-positive cell reaction and to measure its titer. Results Anti-human globulin test cards were used to screen positive specimens with irregular antibody results for antibody specificity; negative samples were tested by the salt water method and the coagglutamine method; negative specimens were tested by the papain method. The positive rate of irregular antibody screening in 1 200 patients was 9 cases, the detection rate was 0.75%, and the negative antibody screening results were false negative in 2 cases. The detection rate is 0.17%. Conclusion For dose-dependent antibodies, try to use homozygous cells for antibody detection. Combined use of anti-screening cells with different antigen patterns and various methods to exclude false negatives from irregular antibody screening can prevent errors and reduce the likelihood of adverse transfusion reactions.
- Antibody screening,
- Blood transfusion,
- Homozygous,
- Heterozygous,
- Low potency antibody

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References

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Significance of excluding anti-screening false negative in blood transfusion patients

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