Volume 21 Issue 12
Dec.  2023
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Article Navigation >  Chinese Journal of General Practice  > 2023  >  21(12): 2045-2048
WEI Xiaofang, CHEN Qiaolin, FENG Youfan, FU Yuan, LIU Fei, ZHANG Wenjie, CHEN Yang, ZHAO Yangyang, HUANG Xiujuan, ZHANG Qike. Functional and mechanistic of TCF3 regulating linc00152 on carcinogenic effects in Burkitt's lymphoma[J]. Chinese Journal of General Practice, 2023, 21(12): 2045-2048. doi: 10.16766/j.cnki.issn.1674-4152.003286
Citation: WEI Xiaofang, CHEN Qiaolin, FENG Youfan, FU Yuan, LIU Fei, ZHANG Wenjie, CHEN Yang, ZHAO Yangyang, HUANG Xiujuan, ZHANG Qike. Functional and mechanistic of TCF3 regulating linc00152 on carcinogenic effects in Burkitt's lymphoma[J]. Chinese Journal of General Practice, 2023, 21(12): 2045-2048. doi: 10.16766/j.cnki.issn.1674-4152.003286
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Functional and mechanistic of TCF3 regulating linc00152 on carcinogenic effects in Burkitt's lymphoma

doi: 10.16766/j.cnki.issn.1674-4152.003286

  • Department of Hematology, Gansu Provincial Hospital, Lanzhou, Gansu 730000, China

Funds:

 21JR1RA023

  • Received Date: 2023-04-18
    Available Online: 2024-01-29
    Abstract
  •   Objective  To investigate the function and mechanism of TCF3 in regulating proliferation and apoptosis of Daudi and Raji cells through linc00152.  Methods  The interference efficiency of linc00152 was detected by real-time quantitative polymerase chain reaction (qPCR). CCK8, Western blotting, and flow cytometry were used to detect the effects of linc00152 on the proliferation and apoptosis of Daudi and Raji cells. ChIP-PCR detected the binding of TCF3 to the promoter region of linc00152. qPCR and Western blotting were used to detect the interference efficiency of TCF3. qPCR was used to detect the effect of TCF3 on the expression level of linc00152.  Results  siRNA successfully interfered with the expression of linc00152 in Daudi and Raji cells. CCK8 results showed that interference of linc00152 could inhibit the proliferation of Daudi and Raji cells, while Western blotting and flow cytometry results showed that interference of linc00152 could promote cell apoptosis (Daudi: 35.30±2.84 vs. 6.95±0.82; Raji: 33.17±2.18 vs. 6.47±0.38). ChIP-PCR results showed that TCF3 could bind to the promoter of linc00152. The results of qPCR and Western blotting showed that both the mRNA and protein levels of TCF3 could be successfully interfered by siRNA. In Daudi and Raji cells, si-TCF3 could reduce the expression of linc00152 (Daudi: 0.509±0.048 vs. 0.906± 0.146; Raji: 0.502±0.052 vs. 0.966±0.103).  Conclusion  Linc00152 can promote the proliferation of Daudi and Raji cells and inhibit apoptosis, which may be related to the regulation of linc00152 by TCF3.

     

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