Volume 22 Issue 1
Jan.  2024
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LIN Haili, DU Xiaokang, WANG Yilu, CAI Chengsong, GAO Jin, PAN Feng. CD38 influences Treg/Th17 balance to promote rheumatoid arthritis[J]. Chinese Journal of General Practice, 2024, 22(1): 18-20. doi: 10.16766/j.cnki.issn.1674-4152.003321
Citation: LIN Haili, DU Xiaokang, WANG Yilu, CAI Chengsong, GAO Jin, PAN Feng. CD38 influences Treg/Th17 balance to promote rheumatoid arthritis[J]. Chinese Journal of General Practice, 2024, 22(1): 18-20. doi: 10.16766/j.cnki.issn.1674-4152.003321
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CD38 influences Treg/Th17 balance to promote rheumatoid arthritis

doi: 10.16766/j.cnki.issn.1674-4152.003321
  • 1.

    Clinical Laboratory, the Affiliated Hospital of Hangzhou Normal University, Hangzhou, Zhejiang 310015, China

Funds:

 81701568

  • Received Date: 2023-04-29
    Available Online: 2024-03-09
    Abstract
  •   Objective  To detect the expression of cluster of differentiation 38 (CD38) and the ratio of regulatory T cells (Tregs) to helper T cells 17 (Th17) among CD4+ T cells in the tissues of rheumatoid arthritis (RA) mouse model, and to explore the mechanism by which CD38 in promoting the development of rheumatoid arthritis.  Methods  The collagen-induced mice arthritis model (CIA) were constructed to detect CD38 protein and mRNA expression in synovial tissue, spleen, and lymph nodes by protein immunoblotting and fluorescent quantitative PCR. The ratio of Th17 cells and Treg cells were analyzed by flow cytometry. After differentiation, naive CD4+ T cells isolated from the spleen of mice were tested for CD38 protein expression and the ratio of Th17 cells to Treg cells was calculated; By polarizing naive CD4+ T cells, the protein expression of PI3K, AKT, p-AKT, mTOR, p-mTOR were detected and the effect of CD38 on PI3K/AKT/ mTOR signaling pathway were analyzed.  Results  CD38 protein expression in synovial tissue, spleen, and lymph nodes in CIA were higher than that in the control group, and the differences were statistically significant (P < 0.01). The percentage of naive CD4+ T cells in specific differentiation conditions, compared with the polarized group+sh-NC, the proportion of Th17 cells in the polarized group+sh-CD38 was decreased, and the proportion of Treg cells was increased (P < 0.01). After the polarization treatment of CD4+ T cells, the expression of p-AKT and p-mTOR proteins in the polarized group+sh-NC (3.00±0.08, 3.18±0.12) were higher than that in polarized group+sh-CD38 (2.48±0.09, 1.70±0.10, P < 0.01).  Conclusion  CD38 is highly expressed in CIA, and inhibition of CD38 expression improves CIA inflammation. Under specific differentiation conditions, high CD38 expression increases the proportion of Th17 cells and decreases the proportion of Treg cells. Through polarization, CD38 affects the balance between Treg and Th17 through the PI3K/AKT/mTOR signaling pathway to promote the development of rheumatoid arthritis inflammation.

     

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