Regulation mechanism of miR-206 on senescence and apoptosis of nucleus pulposus cells in lumbar intervertebral disc

ZHU Zhonglian; ZHOU Pinghui; WANG Zhaodong; GAO Xubin; XU Chen; LIU Yajun; DUAN Keyou; GUAN Jianzhong

doi:10.16766/j.cnki.issn.1674-4152.003367

Volume 22 Issue 2

Feb. 2024

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Article Navigation > Chinese Journal of General Practice > 2024 > 22(2): 209-211

ZHU Zhonglian, ZHOU Pinghui, WANG Zhaodong, GAO Xubin, XU Chen, LIU Yajun, DUAN Keyou, GUAN Jianzhong. Regulation mechanism of miR-206 on senescence and apoptosis of nucleus pulposus cells in lumbar intervertebral disc[J]. Chinese Journal of General Practice, 2024, 22(2): 209-211. doi: 10.16766/j.cnki.issn.1674-4152.003367

Citation:

ZHU Zhonglian, ZHOU Pinghui, WANG Zhaodong, GAO Xubin, XU Chen, LIU Yajun, DUAN Keyou, GUAN Jianzhong. Regulation mechanism of miR-206 on senescence and apoptosis of nucleus pulposus cells in lumbar intervertebral disc[J]. Chinese Journal of General Practice, 2024, 22(2): 209-211. doi: 10.16766/j.cnki.issn.1674-4152.003367

Citation:

ZHU Zhonglian, ZHOU Pinghui, WANG Zhaodong, GAO Xubin, XU Chen, LIU Yajun, DUAN Keyou, GUAN Jianzhong. Regulation mechanism of miR-206 on senescence and apoptosis of nucleus pulposus cells in lumbar intervertebral disc[J]. Chinese Journal of General Practice, 2024, 22(2): 209-211. doi: 10.16766/j.cnki.issn.1674-4152.003367

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Regulation mechanism of miR-206 on senescence and apoptosis of nucleus pulposus cells in lumbar intervertebral disc

doi: 10.16766/j.cnki.issn.1674-4152.003367

ZHU Zhonglian^{1, 2}
, ZHOU Pinghui^{1, 2}
, WANG Zhaodong^{1, 2}
, GAO Xubin^{1, 2}
, XU Chen^{1, 2}
, LIU Yajun^{1, 2}
, DUAN Keyou^{1, 2}
, GUAN Jianzhong^{1, 2
,
,}

1.
Department of Orthopedics, the First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui 233004, China

Funds:

2023AH051966

2022byzd055

Received Date: 2023-08-16
Available Online: 2024-03-27

Abstract

Abstract

Objective To investigate the regulatory effects of miR-206 on senescence and apoptosis of nucleus pulposus cells in white rabbits with lumbar disc herniation, and to provide a reference for targeted therapy of lumbar disc herniation. Methods Thirty cases of New Zealand white rabbits were selected as the study subjects to construct white rabbit model of lumbar disc herniation by autologous nucleus pulposus transplantation. The rabbits were randomly divided into a model group and a control group, with 15 rabbits in each group. The rabbits in the model group were injected with miR-206 simulant, and the rabbits in the control group were injected with normal saline. The transcription level of miR-206 was detected by reverse transcription-polymerase chain reaction (qRT-PCR). Senescence of nucleus pulposus cells was detected by senescence-associated β-galactosidase (SA-β-gal). Nucleus pulposus apoptosis was detected by flow cytometry. Results The expression level of miR-206 in nucleus pulposus cells in the model group (1.65±0.32) was significantly higher than that in the control group (0.41±0.08), the difference was significant (P<0.05). The number of positive cells in the model group[(14.27±5.36) ×10⁵] was higher than that in the control group [(5.08±1.13) ×10⁵], the difference was statistically significant (P<0.05). The apoptosis rate of nucleus pulposus cells in the model group [(8.18±1.94)%] was lower than that in the control group [(42.43±6.28)%], the difference was statistically significant (P<0.05). Conclusion miR-206 plays an important regulatory role in nucleus pulposus cells of white rabbits with lumbar disc herniation. Overexpression of miR-206 can reduce the senescence of nucleus pulposus cells, decrease the apoptosis rate of nucleus pulposus cells in white rabbits with lumbar disc herniation, and improve the proliferation ability of nucleus pulposus cells.
- miR-206,
- Lumbar disc herniation,
- The big white rabbit,
- Nucleus pulposus cells,
- Aging,
- Apoptosis

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References(19)

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Regulation mechanism of miR-206 on senescence and apoptosis of nucleus pulposus cells in lumbar intervertebral disc

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Regulation mechanism of miR-206 on senescence and apoptosis of nucleus pulposus cells in lumbar intervertebral disc

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