Volume 22 Issue 3
Mar.  2024
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Article Navigation >  Chinese Journal of General Practice  > 2024  >  22(3): 418-423
WANG Jinyu, LI Guoping. Mechanism of circ0006152/miR-557/Wnt3a signal axis in the genesis and development of non-small cell lung cancer A549 cells[J]. Chinese Journal of General Practice, 2024, 22(3): 418-423. doi: 10.16766/j.cnki.issn.1674-4152.003416
Citation: WANG Jinyu, LI Guoping. Mechanism of circ0006152/miR-557/Wnt3a signal axis in the genesis and development of non-small cell lung cancer A549 cells[J]. Chinese Journal of General Practice, 2024, 22(3): 418-423. doi: 10.16766/j.cnki.issn.1674-4152.003416
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Mechanism of circ0006152/miR-557/Wnt3a signal axis in the genesis and development of non-small cell lung cancer A549 cells

doi: 10.16766/j.cnki.issn.1674-4152.003416

  • Respiratory Department of Huzhou Central Hospital, Huzhou, Zhejiang 313000, China

Funds:

 2019RC281

  • Received Date: 2023-02-14
    Available Online: 2024-05-27
    Abstract
  •   Objective  To investigate circular RNA is associated with the occurrence and development of non-small cell lung cancer. This study aims to investigate the mechanism of circ0006152/miR-557/Wnt3a signaling axis in the occurrence and development of non-small cell lung cancer A549 cells.  Methods  Take A549 cells in logarithmic growth period for grouping and transfection, and divide them into control group (cells are only routinely cultured), si-NC group (transfected with si-NC), si-circ0006152 group (transfected with si-circ0006152), miR-NC group (transfected with miR-NC), miR-557 mimics group (transfected with miR-557 mimics) and si-circ0006152+miR-557 inhibitors group (transfected with miR-557 inhibitors and si-circ0006152) by random number table method. Proliferation, apoptosis, migration invasion and Wnt3a, beta-catenin protein were detected.  Results  Compared with the si-NC group, the proliferation inhibition rate and apoptosis rate of A549 cells in the si-circ0006152 group were significantly increased, and the number of invasion was significantly decreased (P < 0.001); Compared with the miR-NC group, the proliferation inhibition rate of A549 cells [(48.42 ± 5.73)% vs.(4.27 ± 0.19)%] and apoptosis rate of A549 cells in miR-557 mimics group [(45.38±5.96)% vs. (8.94±2.11)%] were significantly increased. The number of cells invaded (55.42±8.65 vs. 167.42 ± 19.53) was significantly decreased (P < 0.001). The cell proliferation inhibition rate and apoptosis rate in the si-circ0006152+miR-557 inhibitors group were significantly decreased, and the number of cell invasion was significantly increased in the si-circ0006152+miR-557 inhibitors group (P < 0.05). The expressions of Wnt3a and β-catenin proteins in the inhibitors group were significantly increased (P < 0.05).  Conclusion  Silencing circ0006152 can reduce the malignancy of NSCLC A549 cells by regulating the miR-557/Wnt3a signaling axis.

     

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