Volume 22 Issue 3
Mar.  2024
Turn off MathJax
Article Contents
Article Navigation >  Chinese Journal of General Practice  > 2024  >  22(3): 424-428
MA Guojun, LI Yang, FU Jianhua, YANG Dawei. Clinical value of count of circulating tumor cells detection in patients with adenocarcinoma of esophagogastric junction[J]. Chinese Journal of General Practice, 2024, 22(3): 424-428. doi: 10.16766/j.cnki.issn.1674-4152.003417
Citation: MA Guojun, LI Yang, FU Jianhua, YANG Dawei. Clinical value of count of circulating tumor cells detection in patients with adenocarcinoma of esophagogastric junction[J]. Chinese Journal of General Practice, 2024, 22(3): 424-428. doi: 10.16766/j.cnki.issn.1674-4152.003417
shu

Clinical value of count of circulating tumor cells detection in patients with adenocarcinoma of esophagogastric junction

doi: 10.16766/j.cnki.issn.1674-4152.003417
  • 1.

    Department of thoracic Surgery, Liaocheng people ' s Hospital, Liaocheng, Shandong 252000, China

Funds:

 ZR2021MH215

 2022YDSF37

  • Received Date: 2023-07-26
    Available Online: 2024-05-27
    Abstract
  •   Objective  To investigate the relationship between the count of circulating tumor cells (CTCs) in peripheral blood and the clinical characteristics and overall survival (OS) of patients with adenocarcinoma of esophagogastric junction (AEG), and to explore the clinical application value of CTCs detection in AEG patients.  Methods  A total of 154 patients with AEG were eligible for enrollment in Liaocheng People ' s Hospital from July 2016 to June 2020 in the retrospective study. Negative enrichment techniques and fluorescence in situ hybridization (NE-FISH) were used to detect CTCs one week before treatment and the relationship between CTCs count and clinical characteristics of AEG patients was then investigated. Moreover, relationship between CTCs count and OS in prognosis were also explored.  Results  The positive rate of CTCs was 57.1% (32/56) and the positive rates of CEA, CA19-9 and CA72-4 were 19.6% (11/56), 1.8% (1/56) and 12.5% (7/56) respectively in the early AEG patients. The presence of CTCs was significantly correlated with AEG patients, CTCs were positive (≥2) in patients with AEG was significantly correlated with intravascular tumor thrombus (χ2=45.262, P < 0.001), TNM stage (χ2=14.304, P < 0.001)and lymph node metastasis (χ2=5.158, P=0.023). Log-rank univariate analysis showed that CTCs ≥ 2 (χ2=12.486, P < 0.001) and CTCs ≥ 3 (χ2=45.344, P < 0.001) were closely related to OS in AEG patients(P < 0.001). Cox multivariate analysis showed that CTCs ≥ 3 (HR=2.459, 95% CI: 1.472-4.107, P=0.001) was an independent risk factor for the prognosis of OS in AEG patients.  Conclusion  The detection of CTCs by negative enrichment fluorescence in situ hybridization can be used as an auxiliary diagnostic indicator for early AEG patients, which is closely related to the pathological stage of AEG patients and tumor recurrence and metastasis. CTCs ≥ 3 can be used as an independent risk factor for poor prognosis of AEG patients and have a certain clinical application value.

     

    • FullText(HTML)
  • loading
    • References(18)
  • [1]
    SUNG H, FERLAY J, SIEGEL R L, et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. Ca Cancer J Clin, 2021, 71(3): 209-249. doi: 10.3322/caac.21660
    [2]
    WANG H L, ZHAO X K, ZHOU F Y, et al. Characterization of e-cadherin expression in normal mucosa, dysplasia and adenocarcinoma of gastric cardia and its influence on prognosis[J]. World J Gastrointest Oncol, 2022, 14(1): 265-277. doi: 10.4251/wjgo.v14.i1.265
    [3]
    LI Y, MA G, ZHAO P, et al. Improvement of sensitive and specific detection of circulating tumor cells using negative enrichment and immunostaining-fish[J]. Clin Chim Acta, 2018, 485(1): 95-102.
    [4]
    MA G, YANG D, LI Y, et al. Combined measurement of circulating tumor cell counts and serum tumor marker levels enhances the screening efficiency for malignant versus benign pulmonary nodules[J]. Thorac Cancer, 2022, 13(23): 3393-3401. doi: 10.1111/1759-7714.14702
    [5]
    LI Y, WANG Z, FU R, et al. Clinical utility of circulating tumor cells in patients with esophageal cancer[J]. Front Oncol, 2022, 12(2): 60-68.
    [6]
    JELSKI W, MROCZKO B. Molecular and circulating biomarkers of gastric cancer[J]. Int J Mol Sci, 2022, 23(14): 75-88.
    [7]
    MA S, ZHOU M, XU Y, et al. Clinical application and detection techniques of liquid biopsy in gastric cancer[J]. Mol Cancer, 2023, 22(1): 7. doi: 10.1186/s12943-023-01715-z
    [8]
    XU J, LIAO K, YANG X, et al. Using single-cell sequencing technology to detect circulating tumor cells in solid tumors[J]. Mol Cancer, 2021, 20(1): 96-104. doi: 10.1186/s12943-021-01388-6
    [9]
    GALVIS M M, ROMERO C S, BUENO T O, et al. Toward a new era for the management of circulating tumor cells[J]. Adv Exp Med Biol, 2021, 1286(3): 125-134.
    [10]
    MA X, OU K, LIU X, et al. Application progress of liquid biopsy in gastric cancer[J]. Front Oncol, 2022, 12(10): 54-66.
    [11]
    GKOUNTELA S, CASTRO-GINER F, SZCZERBA B M, et al. Circulating tumor cell clustering shapes DNA methylation to enable metastasis seeding[J]. Cell, 2019, 176(2): 98-112.
    [12]
    CHENG B, TONG G, WU X, et al. Enumeration and characterization of circulating tumor cells and its application in advanced gastric cancer[J]. Onco Targets Ther, 2019, 12(6): 7887-7896.
    [13]
    JHI J H, KIM G H, PARK S J, et al. Circulating tumor cells and twist expression in patients with metastatic gastric cancer: a preliminary study[J]. J Clin Med, 2021, 10(19): 70-81.
    [14]
    YANG Y, ZHENG J, LI Y. Comparison of 4 lymph node staging systems for the prognostic prediction of esophagogastric junction adenocarcinoma with ≤ 15 retrieved lymph nodes[J]. Eur J Surg Oncol, 2022, 48(5): 1017-1024. doi: 10.1016/j.ejso.2021.11.133
    [15]
    ZENG C D D, JIN C C, GAO C, et al. Preoperative folate receptor-positive circulating tumor cells are associated with occult peritoneal metastasis and early recurrence in gastric cancer patients: a prospective cohort study[J]. Front Oncol, 2022, 29(12): 191-203.
    [16]
    赵天豪, 杨一群, 李岳彤, 等. 泛素结合酶2S在胃癌中的表达及临床意义[J]. 中华全科医学, 2023, 21(4): 555-559. doi: 10.16766/j.cnki.issn.1674-4152.002929

    ZHAO T H, YANG Y Q, LI Y T, et al. Expression and clinical significance of UBE2S in gastric cancer[J]. Chinese Journal of General Practice, 2023, 21(4): 555-559. doi: 10.16766/j.cnki.issn.1674-4152.002929
    [17]
    SONG X H, LIU K, YANG S J, et al. Prognostic value of changes in preoperative and postoperative serum CA19-9 levels in gastric cancer[J]. Front Oncol, 2020, 10(2): 22-32.
    [18]
    WANG Y W, LI L L, LU M, et al. Stem cell-like circulating tumor cells indicate poor prognosis in gastric cancer[J]. Arch Med Sci, 2022, 18(5): 1297-1307.
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(3)  / Tables(3)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (57) PDF downloads(0) Cited by()
    Proportional views
    Related

    Address：No. 287, Changhuai Road, Bengbu, Anhui Province, 233004, P.R.ChinaphoneNo：0552-3066635； 0552-3051890Email：zhqkyx@163.com

    Copyright © Chinese Journal of General Practice皖ICP备2020018345号-2

    Supported by: Beijing Renhe Information Technology Co., Ltd.    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return