Effect of PPARγ agonists on microglia polarization around hematoma after cerebral hemorrhage in male rats

XU Yingning; MU Qiong

doi:10.16766/j.cnki.issn.1674-4152.003502

Volume 22 Issue 5

May 2024

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XU Yingning, MU Qiong. Effect of PPARγ agonists on microglia polarization around hematoma after cerebral hemorrhage in male rats[J]. Chinese Journal of General Practice, 2024, 22(5): 781-785. doi: 10.16766/j.cnki.issn.1674-4152.003502

Citation:

XU Yingning, MU Qiong. Effect of PPARγ agonists on microglia polarization around hematoma after cerebral hemorrhage in male rats[J]. Chinese Journal of General Practice, 2024, 22(5): 781-785. doi: 10.16766/j.cnki.issn.1674-4152.003502

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Effect of PPARγ agonists on microglia polarization around hematoma after cerebral hemorrhage in male rats

doi: 10.16766/j.cnki.issn.1674-4152.003502

XU Yingning^{1, 2}
, MU Qiong^{3
,
,}

1.
Department of Pathology, Sir Run Run Shaw Hospital Affiliated to Zhejiang University, Hangzhou, Zhejiang 310000, China

Funds:

2023KY123

gyfynsfc[2020]-2

Received Date: 2023-09-22

Abstract

Abstract

Objective A model of intracerebral hemorrhage (ICH) was established to study the effect of PPARγ agonist (rosiglitazone) on the polarization of microglia (M1 and M2) around the hematoma after intracerebral hemorrhage in male SD rats. Methods A total of 48 male SD rats were divided into four groups according to the random number table method, including the normal group (Control), the intracerebral hemorrhage model group (ICH), the sodium chloride intervention group (NaCl), and the rosiglitazone intervention group (RSG), with 12 rats in each group. The ICH model of male rats was established by autologous blood. The neurological function of all groups of male rats was scored at the time points of 6 h, 24 h, 48 h, and 72 h. The expressions of CD16 and CD206 in the peripheral tissues of hematoma were detected by immunohistochemistry. Results The Longa scores of the ICH group were higher than those of the NaCl group at all time points (P<0.05). The Longa scores of the RSG group were lower than those of the ICH group at all time points (P<0.05). At 6 hours after intracerebral hemorrhage modeling, the CD16 level in the RSG group (0.226±0.004) was higher than that in the control group (0.210±0.004), the ICH group (0.221±0.004), and the NaCl group (0.220±0.005) expression was significantly increased, and the differences were statistically significant(P<0.05). The CD206 in the RSG group (0.204±0.004) was significantly higher than that in the control group (0.184±0.005), the ICH group (0.195±0.005), and the NaCl group (0.190±0.006) at 24 hours after intracerebral hemorrhage modeling(P<0.05). Conclusion PPARγ agonist rosiglitazone can enhance the expression of M1 and M2 microglia around hematoma after ICH, especially promoting the transformation of microglia into M2.
- Intracerebral hemorrhage,
- Microglia,
- PPARγ agonist,
- Rosiglitazone,
- Polarization

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References(18)

References

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Effect of PPARγ agonists on microglia polarization around hematoma after cerebral hemorrhage in male rats

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