Role of the transmembrane protein TMEM88 in acute liver injury induced by LPS/D-GaIN

HE Cuixia; ZHU Minhui; XU Yuanyuan; WANG Xuefeng; DING Jiaxiang; ZHOU Huan

doi:10.16766/j.cnki.issn.1674-4152.003503

Volume 22 Issue 5

May 2024

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Article Navigation > Chinese Journal of General Practice > 2024 > 22(5): 786-790

HE Cuixia, ZHU Minhui, XU Yuanyuan, WANG Xuefeng, DING Jiaxiang, ZHOU Huan. Role of the transmembrane protein TMEM88 in acute liver injury induced by LPS/D-GaIN[J]. Chinese Journal of General Practice, 2024, 22(5): 786-790. doi: 10.16766/j.cnki.issn.1674-4152.003503

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HE Cuixia, ZHU Minhui, XU Yuanyuan, WANG Xuefeng, DING Jiaxiang, ZHOU Huan. Role of the transmembrane protein TMEM88 in acute liver injury induced by LPS/D-GaIN[J]. Chinese Journal of General Practice, 2024, 22(5): 786-790. doi: 10.16766/j.cnki.issn.1674-4152.003503

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Role of the transmembrane protein TMEM88 in acute liver injury induced by LPS/D-GaIN

doi: 10.16766/j.cnki.issn.1674-4152.003503

HE Cuixia^{1, 2}
, ZHU Minhui^{1, 2}
, XU Yuanyuan^{1, 2}
, WANG Xuefeng^{1, 2}
, DING Jiaxiang^{1, 3}
, ZHOU Huan^{1, 2, 3
,
,}

1.
Clinical Trials Center, the First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui 233004, China

Funds:

by51201313

Received Date: 2023-02-15
Available Online: 2024-07-20

Abstract

Abstract

Objective This study aims to investigate the changes of TMEM88 expression in mice with acute liver injury induced by lipopolysaccharide (LPS) combined with D-galactosamine (D-GaIN), and investigate the effect of high and low TMEM88 expression on the secretion of inflammatory factors in AML-12 cells induced by LPS. Methods In vivo, 20 mice were randomly divided into normal and model groups with 10 mice in each group according to random number table method. The model group was induced by LPS/D-GaIN combination, and liver tissues and blood of mice were collected. In vitro, AML-12 cells were stimulated by LPS and transfected with TMEM88 to access the changes in the expression of TMEM88 and inflammatory factors. Results The model group exhibited significant liver tissue, with a successful ALI modelling rate of 80%. In addition, the hepatic TMEM88 expression level was significantly increased in the model group (P<0.05). In AML-12 cells, the optimal condition for LPS stimulation was determined to be 100 ng/mL for 24 h. pEGFP-C1-TMEM88 and TMEM88-siRNA were verified to be successfully transfected into LPS-stimulated AML-12 cells (P<0.05). Furthermore, overexpression of TMEM88 resulted in an upregulation of inflammatory factors IL-1β, IL-6, and TNF-α (P<0.01), while knockdown of TMEM88 reduced the secretion of inflammatory factors (P<0.05). These experimental results suggest that the expression level of TMEM88 may influence the inflammatory process. Conclusion The expression level of TMEM88 plays an important role in the LPS/D-GaIN-induced acute liver injury model.
- TMEM88,
- LPS/D-GaIN,
- Acute liver injury,
- Inflammatory factor

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Role of the transmembrane protein TMEM88 in acute liver injury induced by LPS/D-GaIN

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Role of the transmembrane protein TMEM88 in acute liver injury induced by LPS/D-GaIN

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