Volume 22 Issue 5
May  2024
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Article Navigation >  Chinese Journal of General Practice  > 2024  >  22(5): 786-790
HE Cuixia, ZHU Minhui, XU Yuanyuan, WANG Xuefeng, DING Jiaxiang, ZHOU Huan. Role of the transmembrane protein TMEM88 in acute liver injury induced by LPS/D-GaIN[J]. Chinese Journal of General Practice, 2024, 22(5): 786-790. doi: 10.16766/j.cnki.issn.1674-4152.003503
Citation: HE Cuixia, ZHU Minhui, XU Yuanyuan, WANG Xuefeng, DING Jiaxiang, ZHOU Huan. Role of the transmembrane protein TMEM88 in acute liver injury induced by LPS/D-GaIN[J]. Chinese Journal of General Practice, 2024, 22(5): 786-790. doi: 10.16766/j.cnki.issn.1674-4152.003503
shu

Role of the transmembrane protein TMEM88 in acute liver injury induced by LPS/D-GaIN

doi: 10.16766/j.cnki.issn.1674-4152.003503
  • 1.

    Clinical Trials Center, the First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui 233004, China

Funds:

 by51201313

  • Received Date: 2023-02-15
    Available Online: 2024-07-20
    Abstract
  •   Objective  This study aims to investigate the changes of TMEM88 expression in mice with acute liver injury induced by lipopolysaccharide (LPS) combined with D-galactosamine (D-GaIN), and investigate the effect of high and low TMEM88 expression on the secretion of inflammatory factors in AML-12 cells induced by LPS.  Methods  In vivo, 20 mice were randomly divided into normal and model groups with 10 mice in each group according to random number table method. The model group was induced by LPS/D-GaIN combination, and liver tissues and blood of mice were collected. In vitro, AML-12 cells were stimulated by LPS and transfected with TMEM88 to access the changes in the expression of TMEM88 and inflammatory factors.  Results  The model group exhibited significant liver tissue, with a successful ALI modelling rate of 80%. In addition, the hepatic TMEM88 expression level was significantly increased in the model group (P<0.05). In AML-12 cells, the optimal condition for LPS stimulation was determined to be 100 ng/mL for 24 h. pEGFP-C1-TMEM88 and TMEM88-siRNA were verified to be successfully transfected into LPS-stimulated AML-12 cells (P<0.05). Furthermore, overexpression of TMEM88 resulted in an upregulation of inflammatory factors IL-1β, IL-6, and TNF-α (P<0.01), while knockdown of TMEM88 reduced the secretion of inflammatory factors (P<0.05). These experimental results suggest that the expression level of TMEM88 may influence the inflammatory process.  Conclusion  The expression level of TMEM88 plays an important role in the LPS/D-GaIN-induced acute liver injury model.

     

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    • References(21)
  • [1]
    高娟, 伍燕, 郑之峻, 等. 黄芩苷对脂多糖诱导人牙龈成纤维细胞表达炎症因子的影响[J]. 中华全科医学, 2022, 20(12): 2002-2005. doi: 10.16766/j.cnki.issn.1674-4152.002755

    GAN J, WU Y, ZHENG Z J, et al. Effect of baicalin on the expression of inflammatory factors in human gingival fibroblasts induced by lipopolysaccharide[J]. Chinese Journal of General Practice, 2022, 20(12): 2002-2005. doi: 10.16766/j.cnki.issn.1674-4152.002755
    [2]
    蔡艳, 糜大国, 蒋伟, 等. 苦参素对脂多糖诱导的海马神经元凋亡的保护作用[J]. 中华全科医学, 2020, 18(10): 1660-1662, 1765. doi: 10.16766/j.cnki.issn.1674-4152.001586

    CAI Y, MI D G, JIANG W, et al. Protective effect of matrine on hippocampal neuron apoptosis induced by lipopolysaccharide[J]. Chinese Journal of General Practice, 2020, 18(10): 1660-1662, 1765. doi: 10.16766/j.cnki.issn.1674-4152.001586
    [3]
    ZHOU H, ZHU X Y, YAO Y, et al. TMEM88 modulates lipid synthesis and metabolism cytokine by regulating Wnt/β-catenin signaling pathway in non-alcoholic fatty liver disease[J]. Front Pharmacol, 2021, 12: 798735. DOI: 10.3389/fphar.2021.798735.
    [4]
    ZHANG X, WAN J X, KE Z P, et al. TMEM88, CCL14 and CLEC3B as prognostic biomarkers for prognosis and palindromia of human hepatocellular carcinoma[J]. Tumour Biol, 2017, 39(7): 1010428317708900. DOI: 10.1177/1010428317708900.
    [5]
    LI M, SONG K, HUANG X W, et al. GDF-15 prevents LPS and D-galactosamine-induced inflammation and acute liver injury in mice[J]. Int J Mol Med, 2018, 42(3): 1756-1764.
    [6]
    龚红霞, 曾元丁, 苏韫, 等. 大补脾汤联合奥沙利铂对胃癌荷瘤小鼠瘤组织TLR4/MyD88/NF-κB信号通路及下游相关分子的影响[J]. 中国免疫学杂志, 2023, 39(3): 572-577. doi: 10.3969/j.issn.1000-484X.2023.03.021

    GONG H X, ZENG Y D, SU Y, et al. Effects of Da Bupi Decoction combined with oxaliplatin on TLR4/MyD88/NF-κB signaling pathway and downstream related molecules in gastric cancer bearing mice[J]. Chinese Journal of Immunology, 2023, 39(3): 572-577. doi: 10.3969/j.issn.1000-484X.2023.03.021
    [7]
    邵长春. 脂多糖在肝再生及其促进肝癌发生中的作用机制研究[D]. 上海: 中国人民解放军海军军医大学, 2021.

    SHAO C C. Study on the mechanism of lipopolysaccharide in liver regeneration and its promotion of hepatocellular carcinoma[D]. Shanghai: Naval Medical University, 2021.
    [8]
    张一凡. TFF3改善NASH肝损伤的机制及降脂颗粒的调控作用[D]. 上海: 上海中医药大学, 2019.

    ZHANG Y F. Mechanism of TFF3 ameliorating liver injury in NASH and regulation of Jiangzhi granules[D]. Shanghai: Shanghai University of Traditional Chinese Medicine, 2019.
    [9]
    XU S, ZHAO M, WANG Q J, et al. Effectiveness of probiotics and prebiotics against acute liver injury: a meta-analysis[J]. Front Med, 2021, 8: 739337. DOI: 10.3389/fmed.2021.739337.
    [10]
    MEZZANO G, JUANOLA A, CARDENAS A, et al. Global burden of disease: acute-on-chronic liver failure, a systematic review and meta-analysis[J]. Gut, 2022, 71(1): 148-155. doi: 10.1136/gutjnl-2020-322161
    [11]
    GE Y X, WANG C H, HU F Y, et al. New advances of TMEM88 in cancer initiation and progression, with special emphasis on Wnt signaling pathway[J]. J Cell Physiol, 2018, 233(1): 79-87. doi: 10.1002/jcp.25853
    [12]
    ZHANG S H, DAI H T, LI W Y, et al. TMEM116 is required for lung cancer cell motility and metastasis through PDK1 signaling pathway[J]. Cell Death Dis, 2021, 12(12): 1086. doi: 10.1038/s41419-021-04369-1
    [13]
    RAO J W, WU X K, ZHOU X Z, et al. TMEM205 is an independent prognostic factor and is associated with immune cell infiltrates in hepatocellular carcinoma[J]. Front Genet, 2020, 11: 575776. DOI: 10.3389/fgene.2020.575776.
    [14]
    ZHAO X, LI G, CHONG T, et al. TMEM88 exhibits an antiproliferative and anti-invasive effect in bladder cancer by downregulating Wnt/β-catenin signaling[J]. J Biochem Mol Toxicol, 2021, 35(8): e22835. DOI: 10.1002/jbt.22835.
    [15]
    GENG Q Q, CHEN X, CHEN N Z. Transmembrane protein 88 exerts a tumor-inhibitory role in thyroid cancer through restriction of Wnt/β-catenin signaling[J]. Exp Cell Res, 2020, 395(2): 112193. DOI: 10.1016/j.yexcr.2020.112193.
    [16]
    CAI M, NI W J, WANG Y H, et al. Targeting TMEM88 as an attractive therapeutic strategy in malignant tumors[J]. Front Oncol, 2022, 12: 906372. DOI: 10.3389/fonc.2022.906372.
    [17]
    XU T, PAN L X, GE Y X, et al. TMEM88 mediates inflammatory cytokines secretion by regulating JNK/P38 and canonical Wnt/β-catenin signaling pathway in LX-2 cells[J]. Inflammopharmacology, 2018, 26(5): 1339-1348. doi: 10.1007/s10787-017-0419-z
    [18]
    LI L Y, YANG C C, LI S W, et al. TMEM88 modulates the secretion of inflammatory factors by regulating YAP signaling pathway in alcoholic liver disease[J]. Inflamm Res, 2020, 69(8): 789-800. doi: 10.1007/s00011-020-01360-y
    [19]
    LEE H, EVANS T. TMEM88 inhibits Wnt signaling by promoting Wnt signalosome localization to multivesicular bodies[J]. iScience, 2019, 19: 267-280. doi: 10.1016/j.isci.2019.07.039
    [20]
    SUN Z M, NING Q, LI H, et al. Transmembrane protein 88 inhibits transforming growth factor-β1-induced-extracellular matrix accumulation and epithelial-mesenchymal transition program in human pleural mesothelial cells through modulating TGF-β1/Smad pathway[J]. J Recept Signal Transduct Res, 2022, 42(1): 60-66. doi: 10.1080/10799893.2020.1843493
    [21]
    XU T, PAN L X, LI L Y, et al. MicroRNA-708 modulates hepatic stellate cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88[J]. J Cell Mol Med, 2020, 24(13): 7127-7140. doi: 10.1111/jcmm.15119
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