Effects of exosomes derived from hepatocellular carcinoma cells on their biological behaviors of proliferation, apoptosis and autophagy

GAO Jie; LI Jiangyan; HUANG Hua; ZHANG Lunjun; DENG Rong

doi:10.16766/j.cnki.issn.1674-4152.003538

Volume 22 Issue 6

Jun. 2024

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Article Navigation > Chinese Journal of General Practice > 2024 > 22(6): 936-939

GAO Jie, LI Jiangyan, HUANG Hua, ZHANG Lunjun, DENG Rong. Effects of exosomes derived from hepatocellular carcinoma cells on their biological behaviors of proliferation, apoptosis and autophagy[J]. Chinese Journal of General Practice, 2024, 22(6): 936-939. doi: 10.16766/j.cnki.issn.1674-4152.003538

Citation:

GAO Jie, LI Jiangyan, HUANG Hua, ZHANG Lunjun, DENG Rong. Effects of exosomes derived from hepatocellular carcinoma cells on their biological behaviors of proliferation, apoptosis and autophagy[J]. Chinese Journal of General Practice, 2024, 22(6): 936-939. doi: 10.16766/j.cnki.issn.1674-4152.003538

Citation:

GAO Jie, LI Jiangyan, HUANG Hua, ZHANG Lunjun, DENG Rong. Effects of exosomes derived from hepatocellular carcinoma cells on their biological behaviors of proliferation, apoptosis and autophagy[J]. Chinese Journal of General Practice, 2024, 22(6): 936-939. doi: 10.16766/j.cnki.issn.1674-4152.003538

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Effects of exosomes derived from hepatocellular carcinoma cells on their biological behaviors of proliferation, apoptosis and autophagy

doi: 10.16766/j.cnki.issn.1674-4152.003538

GAO Jie¹
, LI Jiangyan^{1
,
,}
, HUANG Hua²
, ZHANG Lunjun¹
, DENG Rong¹

1.
Department of Clinical Laboratory, the First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui 233004, China

Funds:

82200661

2020byzd059

2020byzd029

Received Date: 2024-03-04
Available Online: 2024-07-22

Abstract

Abstract

Objective Exosomes play an important role in the occurrence and development of hepatocellular carcinoma. This study aims to investigate the effects of exosomes on the proliferation, apoptosis, autophagy, and exosome release of HepG₂ hepatocellular carcinoma cells and the related mechanisms. Methods Exosomes of HepG₂ cells were extracted by ultrafast centrifugation. Exosome morphology, diameter distribution and marker protein expression of HepG₂ cells were analyzed by transmission electron microscopy, nanoparticle tracking and Western blotting analysis. After the co-culture of exosomes and HepG₂ cells, a CCK-8 kit was used to detect the proliferation of HepG₂ cells, the apoptosis of HepG₂ cells was detected by flow cytometry, and the expressions of apoptosis-related proteins (Bax, Bcl-2) and autophagy-related proteins (LC3-Ⅱ/LC3-Ⅰ, p62, Beclin-1) were detected by Western blotting. Results The morphology of exosomes extracted from HepG₂ cells was consistent with the characteristics of exosomes, and the diameter between 30 and 150 nm. The marker proteins Alix and CD63 were significantly expressed. The results of CCK-8 showed that the absorbance of HepG₂ cells in the 24 h exosome group was not significantly different from that in the control group(P > 0.05), but the absorbance of the exosome group at 48 hours and 72 hours were higher than that of the control group (P < 0.05). Flow cytometry showed that the apoptosis rate was (14.96±0.28) % in the control group and (11.16±0.50) % in the exosome group, and the apoptosis rate decreased (t=11.485, P < 0.001). Western blotting results showed that compared with the control group, the expression of apoptosis-related protein Bcl-2 in exosome group was up-regulated (P < 0.01), and the expression of Bax was down-regulated (P < 0.01). Autophagy associated protein LC3-Ⅱ/LC3-Ⅰ expression was up-regulated (P < 0.01), Beclin-1 expression was up-regulated (P < 0.05), and p62 expression was down-regulated (P < 0.01). Conclusion Exosomes derived from HepG₂ cells can promote cell proliferation and inhibit apoptosis by inducing up-regulation of autophagy.
- Hepatocellular carcinoma cells,
- Exosome,
- Proliferation,
- Apoptosis,
- Autophagy

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References(22)

References

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Effects of exosomes derived from hepatocellular carcinoma cells on their biological behaviors of proliferation, apoptosis and autophagy

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