Volume 22 Issue 6
Jun.  2024
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Article Navigation >  Chinese Journal of General Practice  > 2024  >  22(6): 951-956
FANG Quanquan, QU Ziye, XIE Jingzhi, ZONG Juan, ZHOU Dongmei, YIN Songlou, YIN Hanqiu. Expression and function of IL-22 in rheumatoid arthritis-associated interstitial lung disease[J]. Chinese Journal of General Practice, 2024, 22(6): 951-956. doi: 10.16766/j.cnki.issn.1674-4152.003542
Citation: FANG Quanquan, QU Ziye, XIE Jingzhi, ZONG Juan, ZHOU Dongmei, YIN Songlou, YIN Hanqiu. Expression and function of IL-22 in rheumatoid arthritis-associated interstitial lung disease[J]. Chinese Journal of General Practice, 2024, 22(6): 951-956. doi: 10.16766/j.cnki.issn.1674-4152.003542
shu

Expression and function of IL-22 in rheumatoid arthritis-associated interstitial lung disease

doi: 10.16766/j.cnki.issn.1674-4152.003542

  • Department of Rheumatology and Immunology, the Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221004, China

Funds:

 SJCX22_1274

 KC21157

  • Received Date: 2023-12-11
    Available Online: 2024-07-22
    Abstract
  •   Objective  This study aims to observe the expression of IL-22 in patients with rheumatoid arthritis-associated interstitial lung disease(RA-ILD)and establish an experimental model to explore its role in pulmonary fibrosis.  Methods  We selected 33 patients with rheumatoid arthritis with or without interstitial lung disease, who were newly diagnosed and treated at the Affiliated Hospital of Xuzhou Medical University from October 2021 to October 2022. Additionally, 14 healthy subjects were chosen as controls during the same period. ELISA was used to detect the peripheral blood IL-22 levels in the three groups, and a comparison was made among them. Spearman correlation test was employed to analyze the correlation between IL-22 and lung HRCT scores. Logistic regression was used to analyze risk factors for RA-ILD and to draw ROC curves. Wild mice (WT) and IL-22 knock-out mice (IL-22KO) were treated with normal saline (NS) and Bleomycin (BLM). Following modeling, mice were given intraperitoneal injections of IL-22. HE and Masson staining were used to observe the histopathological changes in the lung tissues. Relevant markers were detected by RT-qPCR and Western blotting.  Results  The levels of IL-22 were higher in the RA-NILD group compared to the RA-ILD group and the healthy control group. There was a negative correlation between IL-22 levels and lung HRCT scores (r=-0.940, P < 0.05). Logistic regression analysis suggested that a low level of IL-22 was an independent risk factor for RA-ILD. The combined diagnosis of RA-ILD with IL-22, age, smoking, APCA, and MCHC had an AUC of 0.959 (95% CI: 0.916-1.000), with a sensitivity of 97.0% and specificity of 87.9%. HE and Masson staining found that the collagen deposition in the lungs of BLM groups were increased compared with the NS groups, accompanied by Szapiel ' s scores and Ashcroft scores. The mRNA and protein expression of fibrosis-related genes (Collagen Ⅰ, Vimentin, and α-SMA) were increased in the BLM groups compared to the NS group, and the IL-22KO group showed even higher levels (P < 0.05). Treatment with IL-22 resulted in a reduction in collagen deposition, mRNA, and protein expression of fibrosis-related genes (P < 0.05).  Conclusion  IL-22 exhibits potential as a protective effect against pulmonary fibrosis, suggesting it may be a potential therapeutic target and clinical predictor in the future interventions.

     

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    • References(22)
  • [1]
    CHEN R X, ZHAO L D, XIAO X Y, et al. Distinctive clinical characteristics and outcome of ILD-onset rheumatoid arthritis and ACPA-positive ILD: a longitudinal cohort of 282 cases[J]. Clin Rev Allergy Immunol, 2021, 60(1): 46-54. doi: 10.1007/s12016-020-08819-0
    [2]
    FIGUS F A, PIGA M, AZZOLIN I, et al. Rheumatoid arthritis: extra-articular manifestations and comorbidities[J]. Autoimmun Rev, 2021, 20(4): 102776. DOI: 10.1016/j.autrev.2021.102776.
    [3]
    HUANG H, CHEN R, SHAO C, et al. Diffuse lung involvement in rheumatoid arthritis: a respiratory physician's perspective[J]. Chin Med J (Engl), 2023, 136(3): 280-286. doi: 10.1097/CM9.0000000000002577
    [4]
    朱彩侠, 田豆豆, 鱼云霞, 等. KL-6在类风湿关节炎相关肺间质病患者中的表达[J]. 中华全科医学, 2023, 21(2): 247-249, 308. doi: 10.16766/j.cnki.issn.1674-4152.002856

    ZHU C X, TIAN D D, YU Y X, et al. Expression of KL-6 in patients with rheumatoid arthritis-associated interstitial lung disease[J]. Chinese Journal of General Practice, 2023, 21(2): 247-249, 308. doi: 10.16766/j.cnki.issn.1674-4152.002856
    [5]
    AKIYAMA M, KANEKO Y. Pathogenesis, clinical features, and treatment strategy for rheumatoid arthritis-associated interstitial lung disease[J]. Autoimmun Rev, 2022, 21(5): 103056. DOI: 10.1016/j.autrev.2022.103056.
    [6]
    OUYANG W, O'GARRA A. IL-10 family cytokines IL-10 and IL-22: from basic science to clinical translation[J]. Immunity, 2019, 50(4): 871-891. doi: 10.1016/j.immuni.2019.03.020
    [7]
    SAXTON R A, HENNEBERG L T, CALAFIORE M, et al. The tissue protective functions of interleukin-22 can be decoupled from pro-inflammatory actions through structure-based design[J]. Immunity, 2021, 54(4): 660-672. e9. doi: 10.1016/j.immuni.2021.03.008
    [8]
    ALETAHA D, NEOGI T, SILMAN A J, et al. 2010 Rheumatoid arthritis classification criteria: an American College of Rheumatology/European League Against Rheumatism collaborative initiative[J]. Arthritis Rheum, 2010, 62(9): 2569-2581. doi: 10.1002/art.27584
    [9]
    TRAVIS W D, COSTABEL U, HANSELL D M, et al. An official American Thoracic Society/European Respiratory Society statement: Update of the international multidisciplinary classification of the idiopathic interstitial pneumonias[J]. Am J Respir Crit Care Med, 2013, 188(6): 733-748. doi: 10.1164/rccm.201308-1483ST
    [10]
    HELBICH T H, HEINZ-PEER G, EICHLER I, et al. Cystic fibrosis: CT assessment of lung involvement in children and adults[J]. Radiology, 1999, 213(2): 537-544. doi: 10.1148/radiology.213.2.r99nv04537
    [11]
    JUGE P A, GRANGER B, DEBRAY M P, et al. A risk score to detect subclinical rheumatoid arthritis-associated interstitial lung disease[J]. Arthritis Rheumatol, 2022, 74(11): 1755-1765. doi: 10.1002/art.42162
    [12]
    HYLDGAARD C, ELLINGSEN T, HILBERG O, et al. Rheumatoid arthritis-associated interstitial lung disease: clinical characteristics and predictors of mortality[J]. Respiration, 2019, 98(5): 455-460. doi: 10.1159/000502551
    [13]
    MARKOVICS A, ROSENTHAL K S, MIKECZ K, et al. Restoring the balance between pro-inflammatory and anti-inflammatory cytokines in the treatment of rheumatoid arthritis: new insights from animal models[J]. Biomedicines, 2022, 10(1): 44.
    [14]
    KULSHRESTHA R, DHANDA H, PANDEY A, et al. Immunopathogenesis and therapeutic potential of macrophage influx in diffuse parenchymal lung diseases[J]. Expert Rev Respir Med, 2020, 14(9): 917-928. doi: 10.1080/17476348.2020.1776117
    [15]
    WANG J, GAO S, ZHANG J, et al. Interleukin-22 attenuates allergic airway inflammation in ovalbumin-induced asthma mouse model[J]. BMC Pulm Med, 2021, 21(1): 385. doi: 10.1186/s12890-021-01698-x
    [16]
    WU Y, MIN J, GE C, et al. Interleukin 22 in liver injury, inflammation and cancer[J]. Int J Biol Sci, 2020, 16(13): 2405-2413. doi: 10.7150/ijbs.38925
    [17]
    TANG R, XIAO X, LU Y, et al. Interleukin-22 attenuates renal tubular cells inflammation and fibrosis induced by TGFβ1 through Notch1 signaling pathway[J]. Ren Fail, 2020, 42(1): 381-390. doi: 10.1080/0886022X.2020.1753538
    [18]
    FANG S, JU D, LIN Y, et al. The role of interleukin-22 in lung health and its therapeutic potential for COVID-19[J]. Front Immunol, 2022, 13: 951107. DOI: 10.3389/fimmu.2022.951107.
    [19]
    GU P, WANG D, ZHANG J, et al. Protective function of interleukin-22 in pulmonary fibrosis[J]. Clin Transl Med, 2021, 11(8): e509. DOI: 10.1002/ctm2.509.
    [20]
    BENDSTRUP E, MOLLER J, KRONBORG-WHITE S, et al. Interstitial lung disease in rheumatoid arthritis remains a challenge for clinicians[J]. J Clin Med, 2019, 8(12): 2038. DOI: 10.3390/jcm8122038.
    [21]
    ZHANG M, YIN J, ZHANG X. Factors associated with interstitial lung disease in patients with rheumatoid arthritis: a systematic review and meta-analysis[J]. PLoS One, 2023, 18(6): e0286191. DOI: 10.1371/journal.pone.0286191.
    [22]
    梅永君, 费艳芳, 王健, 等. 类风湿关节炎相关自身抗体与并发间质性肺病的相关性研究[J]. 中华全科医学, 2020, 18(5): 720-722. doi: 10.16766/j.cnki.issn.1674-4152.001338

    MEI Y J, FEI Y F, WANG J, et al. Study on the association between rheumatoid arthritis autoantibodies and interstitial lung disease[J]. Chinese Journal of General Practice, 2020, 18(5): 720-722. doi: 10.16766/j.cnki.issn.1674-4152.001338
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