Volume 22 Issue 8
Aug.  2024
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Article Navigation >  Chinese Journal of General Practice  > 2024  >  22(8): 1390-1394
DAN Gang, SUN Wei, CHEN Qinghong, HU Lina, GUO Zhengbin, ZHANG Jianhua, ZHANG Rui, LIU Yuan. Establishment and application of standard operation procedure for the entire process of lupus anticoagulant DRVVT detection[J]. Chinese Journal of General Practice, 2024, 22(8): 1390-1394. doi: 10.16766/j.cnki.issn.1674-4152.003643
Citation: DAN Gang, SUN Wei, CHEN Qinghong, HU Lina, GUO Zhengbin, ZHANG Jianhua, ZHANG Rui, LIU Yuan. Establishment and application of standard operation procedure for the entire process of lupus anticoagulant DRVVT detection[J]. Chinese Journal of General Practice, 2024, 22(8): 1390-1394. doi: 10.16766/j.cnki.issn.1674-4152.003643
shu

Establishment and application of standard operation procedure for the entire process of lupus anticoagulant DRVVT detection

doi: 10.16766/j.cnki.issn.1674-4152.003643

  • Department of Laboratory Medicine, the General Hospital of Western Theater Command PLA, Chengdu, Sichuan 610083, China

Funds:

 2021YJ0191

  • Received Date: 2024-03-18
    Available Online: 2024-11-19
    Abstract
  •   Objective   To investigate the standardized procedure for the entire process of lupus anticoagulant (LA) detection using the dilute Russell viper venom time (DRVVT) method to ensure the accuracy of LA test results and to aid in clinical diagnosis and treatment.   Methods   This study followed relevant industry requirements, including "Management Measures for Medical Institution Laboratory Management" and "Quality Requirements for Routine Analysis of Clinical Hematology Tests WS/T 406-2012". The entire process of LA detection using the DRVVT method, including pretest, test and posttest phases stages, was standardized. A "DRVVT Method Lupus Anticoagulant Detection Manual" was created based on standard operating procedures (SOP), and test results were tracked and monitored throughout the process to ensure the accuracy of LA test results and to aid in clinical diagnosis and treatment. The study included a total of 3 595 specimens for LA testing in General Hospital of Western Theater Command PLA, from 2021 to 2023. By analyzing aspects such as the number of specimens submitted, the distribution of submitting departments and annual growth, the study examined the degree of clinical recognition of this project and the improvement in the accuracy of disease diagnosis after establishing a standardized testing process.   Results   By establishing a set of standardized operation and detection procedures, we can effectively monitor the test results, and accurately reflect the concentration of LA in patients, to provide effective support for diagnosis and treatment. With the continuous implementation of standardized procedures, the clinical recognition of relevant items has been improved, leading to a sustained increase in the number of specimens and departments submitting specimen. The number of specimens increased from 841 in 2021 to 1 460 in 2023, with a growth rate of 73.0%. In terms of departments submitting specimen, there were 16 departments in 2021 and this number reached 29 departments in 2023, with a growth rate of 81.3%. This has fully achieved the desired effect of supporting the clinic and actively interacting with the clinical department.   Conclusion   Establishing a standard process for the detection of LA can provide accurate and truthful detection results to the clinical, deepen communication with the clinical on related diseases, and play a positive role in supporting the diagnosis and treatment of related diseases.

     

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