Volume 22 Issue 9
Sep.  2024
Turn off MathJax
Article Contents
Article Navigation >  Chinese Journal of General Practice  > 2024  >  22(9): 1504-1507
SU Shiyue, WEI Zhaolian. Histopathology and changes in CXCL9 expression levels in patients with missed miscarriage[J]. Chinese Journal of General Practice, 2024, 22(9): 1504-1507. doi: 10.16766/j.cnki.issn.1674-4152.003669
Citation: SU Shiyue, WEI Zhaolian. Histopathology and changes in CXCL9 expression levels in patients with missed miscarriage[J]. Chinese Journal of General Practice, 2024, 22(9): 1504-1507. doi: 10.16766/j.cnki.issn.1674-4152.003669
shu

Histopathology and changes in CXCL9 expression levels in patients with missed miscarriage

doi: 10.16766/j.cnki.issn.1674-4152.003669

  • Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China

Funds:

 202104j07020036

  • Received Date: 2024-01-22
    Abstract
  •   Objective  To analyze the histopathological and gene expression changes of villus in patients with missed miscarriage, and explore the mechanism of missed miscarriage.  Methods  Patients with induced abortion from December 2020 to February 2023 from the First Affiliated Hospital of Anhui Medical University were selected to participate in the study, including 35 cases of missed abortion in the study group and 35 cases in the control group. The pathological changes of villus tissue were observed by HE staining and transmission electron microscopy, and the sequencing results and CXCL9 gene expression changes were verified by RNA sequencing and qRT-PCR.  Results  In the study group, the number of HE chromatozytial trophoblasts decreased and inflammatory cells infiltrated, and fluoroscopy electron microscopy showed that the endoplasmic reticulum of cytotrophoblasts expanded, the number of mitochondria in syncytial trophoblast decreased and the number of Hofbauer cells increased. The most significant up-regulated genes were KRT34 (FDR=0.008), MRGPGR (FDR=0.048), CUZD1 (FDR=0.002), CXCL9 (FDR < 0.001), CD72 (FDR=0.043), FGF14 (FDR=0.042), TMEM176A (FDR < 0.001), and GAPT (FDR=0.026). qRT-PCR showed that the expression of KRT34, MRGPGR, CUZD1, CXCL9, CD72, FGF14, TMEM176A and GAPT genes was up-regulated, and the all P < 0.05, while the mRNA expression of CXCL9 in the study group (28.66±0.38) and the control group (27.46±0.75) were significantly different (P < 0.001).  Conclusion  Inflammatory cell infiltration and up-regulation of CXCL9 expression may play an important role in the development of missed miscarriage.

     

    • FullText(HTML)
  • loading
    • References(22)
  • [1]
    MAHMOODINASAB M, LORIPOOR M, VAZIRINEJAD R, et al. Effect of misoprostol with and without evening primrose (Oenothera biennis) on induction of missed abortion[J]. Avicenna J Phytomed, 2023, 13(5): 454-462.
    [2]
    LI Y, LIU X, SUN Y, et al. The expression of PDCD4 in patients with missed abortion and its clinical significance[J]. Reprod Sci, 2017, 24(11): 1512-1519. doi: 10.1177/1933719117692044
    [3]
    WANG S, LI M, SUN F, et al. Th17/Treg-cell balance in the peripheral blood of pregnant females with a history of recurrent spontaneous abortion receiving progesterone or cyclosporine A[J]. Exp Ther Med, 2021, 21(1): 37. DOI: 10.3892/etm.2020.9469.
    [4]
    FANG Y, KONG B, YANG Q, et al. MDM2 309 polymorphism is associated with missed abortion[J] Hum Reprod, 2009, 24(6): 1346-1349. doi: 10.1093/humrep/dep044
    [5]
    OZAWA N, OGAWA K, SASAKI A, et al. Maternal age, history of miscarriage, and embryonic/fetal size are associated with cytogenetic results of spontaneous early miscarriages[J]. J Assist Reprod Genet, 2019, 36(4): 749-757. doi: 10.1007/s10815-019-01415-y
    [6]
    LA ROCCA C, CARBONE F, LONGOBARDI S, et al. The immunology of pregnancy: regulatory T cells control maternal immune tolerance toward the fetus[J]. Immunol Lett, 2014, 162(1 Pt A): 41-48.
    [7]
    JURKOVIC D, OVERTON C, BENDER-ATIK R. Diagnosis and management of first trimester miscarriage[J]. BMJ, 2013, 346: f3676. DOI: 10.1136/bmj.f3676.
    [8]
    GRAZIOSI G C, MOL B W, REUWER P J, et al. Misoprostol versus curettage in women with early pregnancy failure after initial expectant management: a randomized trial[J]. Hum Reprod, 2004, 19(8): 1894-1899. doi: 10.1093/humrep/deh344
    [9]
    CAMERON S. Recent advances in improving the effectiveness and reducing the complications of abortion[J]. F1000Res, 2018, 7: F1000 Faculty Rev-1881. DOI: 10.12688/f1000research.15441.1.
    [10]
    XUE H, GUO Q, YU A, et al. Genetic analysis of chorionic villus tissues in early missed abortions[J]. Sci Rep, 2023, 13(1): 21719. DOI: 10.1038/s41598-023-48358-0.
    [11]
    谢幸, 孔北华, 段涛. 妇产科学[M]. 9版. 北京: 人民卫生出版社, 2018: 72-73.

    XIE X, KONG B H, DUAN T. Obstetrics and gynecology[M]. 9th edition. Beijing: People's Medical Publishing House, 2018: 72-73.
    [12]
    LINNAKAARI R, HELLE N, MENTULA M, et al. Trends in the incidence, rate and treatment of miscarriage-nationwide register-study in Finland, 1998-2016[J]. Hum Reprod, 2019, 34(11): 2120-2128.
    [13]
    HOMER H A. Modern management of recurrent miscarriage[J]. Aust N Z J Obstet Gynaecol, 2019, 59(1): 36-44. doi: 10.1111/ajo.12920
    [14]
    CHEN X, MAN GCW, LIU Y, et al. Physiological and pathological angiogenesis in endometrium at the time of embryo implantation[J]. Am J Reprod Immunol, 2017, 78(2). DOI: 10.1111/aji.12693.
    [15]
    ZHI Z, YANG W, LIU L, et al. Early missed abortion is associated with villous angiogenesis via the HIF-1α/VEGF signaling pathway[J]. Arch Gynecol Obstet, 2018, 298(3): 537-543. doi: 10.1007/s00404-018-4802-9
    [16]
    XIA S, ZHEN Y, MA H, et al. Abnormal expression of microRNA-575 leads to missed abortion through regulating apoptosis and angiogenesis[J]. Exp Ther Med, 2017, 14(5): 3993-4000.
    [17]
    SEVAL Y, KORGUN ET, DEMIR R. Hofbauer cells in early human placenta: possible implications in vasculogenesis and angiogenesis[J]. Placenta, 2007, 28(8-9): 841-845. doi: 10.1016/j.placenta.2007.01.010
    [18]
    WETZKA B, CLARK D E, CHARNOCK-JONES D S, et al. Isolation of macrophages (Hofbauer cells) from human term placenta and their prostaglandin E2 and thromboxane production[J]. Hum Reprod, 1997, 12(4): 847-852. doi: 10.1093/humrep/12.4.847
    [19]
    RIZOV M, ANDREEVA P, DIMOVA I. Molecular regulation and role of angiogenesis in reproduction[J]. Taiwan J Obstet Gynecol, 2017, 56(2): 127-132. doi: 10.1016/j.tjog.2016.06.019
    [20]
    陈霄, 吴小颖, 安明. 血清CXC趋化因子配体14和肿瘤坏死因子超家族成员13水平对卵巢子宫内膜异位症患者术后复发的预测价值[J]. 中国医药, 2023, 18(9): 1366-1370.

    CHEN X, WU X Y, AN M. Predictive value of serum CXC chemokine ligand 14 and tumor necrosis factor superfamily member 13 in postoperative recurrence in patients with ovarian endometriosis[J]. China Medicine, 2023, 18(9): 1366-1370.
    [21]
    PALOMINO D C, MARTI L C. Chemokines and immunity[J]. Einstein (Sao Paulo), 2015, 13(3): 469-473.
    [22]
    高骏, 蒋雪生, 盛翔, 等. 活动期强直性脊柱炎患者外周血单个核细胞CXCL16 mRNA和CXCL6 mRNA的表达及CXCL16对淋巴细胞增殖的影响[J]. 中华全科医学, 2017, 15(8): 1348-1350. doi: 10.16766/j.cnki.issn.1674-4152.2017.08.022

    GAO J, JIANG X S, SHENG X, et al. Expression of CXCL16 mRNA and CXCL6 mRNA in peripheral blood mononuclear cells of patients with active ankylosing spondylitis and its effect on lymphocyte proliferation[J]. Chinese Journal of General Practice, 2017, 15(8): 1348-1350. doi: 10.16766/j.cnki.issn.1674-4152.2017.08.022
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(5)  / Tables(1)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (34) PDF downloads(2) Cited by()
    Proportional views
    Related

    Address：No. 287, Changhuai Road, Bengbu, Anhui Province, 233004, P.R.ChinaphoneNo：0552-3066635； 0552-3051890Email：zhqkyx@163.com

    Copyright © Chinese Journal of General Practice皖ICP备2020018345号-2

    Supported by: Beijing Renhe Information Technology Co., Ltd.    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return