Volume 22 Issue 10
Oct.  2024
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Article Navigation >  Chinese Journal of General Practice  > 2024  >  22(10): 1684-1686
LU Xiufang, KANG Yali, LI Xia, HU Jinding, TIAN Jinjing. The gene characteristics of carbapenem-resistant enterobacteriaceae in patients with urinary tract infection[J]. Chinese Journal of General Practice, 2024, 22(10): 1684-1686. doi: 10.16766/j.cnki.issn.1674-4152.003711
Citation: LU Xiufang, KANG Yali, LI Xia, HU Jinding, TIAN Jinjing. The gene characteristics of carbapenem-resistant enterobacteriaceae in patients with urinary tract infection[J]. Chinese Journal of General Practice, 2024, 22(10): 1684-1686. doi: 10.16766/j.cnki.issn.1674-4152.003711
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The gene characteristics of carbapenem-resistant enterobacteriaceae in patients with urinary tract infection

doi: 10.16766/j.cnki.issn.1674-4152.003711

  • Department of Clinical Laboratory, the Second People ' s Hospital of Liaocheng, Liaocheng, Shandong 252600, China

Funds:

 202211000916

  • Received Date: 2024-03-23
    Available Online: 2024-12-28
    Abstract
  •   Objective   To explore the drug resistance and gene characteristics of carbapenem-resistant Enterobacteriaceae (CRE) in patients with urinary tract infection, and to provide reference for the prevention and treatment of CRE in this area.   Methods   Clinical data of 81 strains of CRE isolated from urinary tract infection patients in the Second People ' s Hospital of Liaocheng from January 2017 to December 2022 were collected, and their drug resistance and resistance gene characteristics were analyzed.   Results   Among the 81 strains of CRE, Escherichia coli accounted for 55.56% (45/81) and Klebsiella pneumoniae accounted for 28.40% (23/81). The drug resistance rates of CRE to polycolistin and tigecycline were lower [4.94% (4/81), 0]. The resistance rate of Escherichia coli to ceftazidime/avibactam was as high as 84.44% (38/45), while the resistance rate of Klebsiella pneumoniae to ceftazidime/avibactam was 8.70% (2/23). Among 81 CRE strains, 22 were blaKPC, and all were blaKPC-2. There were 59 strains of blaNDM, including 57 blaNDM-5 and 2 blaNDM-1. The main resistance gene of Escherichia coli was blaNDM-5 [97.78% (44/45)], and that of Klebsiella pneumoniae was blaKPC-2 [95.65% (22/23)]. ST11 dominated the ST type of 23 strains of carbapenem-resistant Klebsiella pneumoniae [CRKP, 86.96% (20/23)], and ST410 dominated the ST type of 45 strains of carbapenem-resistant Escherichia coli [CREC, 51.11% (23/45)].   Conclusion   The resistance rate of CRE in patients with urinary tract infection was higher, blaNDM-5 was the main resistance gene of Escherichia coli, blaKPC-2 was the main resistance gene of Klebsiella pneumoniae, and ST11 was the main ST type.

     

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    • References(24)
  • [1]
    茹硕琦, 杨建坤, 滕菲, 等. 耐碳青霉烯类肠杆菌目细菌感染治疗药物及治疗策略研究进展[J]. 中国感染与化疗杂志, 2023, 23(4): 518-523.

    RU S Q, YANG J K, TENG F, et al. Latest researches on the antimicrobial agents and treatment strategy for the infections caused by carbapenem-resistant Enterobacterales[J]. Chinese Journal of Infection and Chemotherapy, 2023, 23(4): 518-523.
    [2]
    周奋, 金雨虹, 王广芬, 等. 重症监护病房耐碳青霉烯类肠杆菌科细菌感染的危险因素分析[J]. 中华全科医学, 2019, 17(4): 580-582, 648. doi: 10.16766/j.cnki.issn.1674-4152.000740

    ZHOU F, JIN Y H, WANG G F, et al. Risk factors of carbapenem-resistant enterobacteriaceae infection in intensive care unit[J]. Chinese Journal of General Practice, 2019, 17(4): 580-582, 648. doi: 10.16766/j.cnki.issn.1674-4152.000740
    [3]
    王双双, 李亚玲, 陈丽琴, 等. 儿童重症监护病区CRE感染的危险因素及耐药情况分析[J]. 昆明医科大学学报, 2023, 44(9): 49-54.

    WANG S S, LI Y L, CHEN L Q, et al. The Analysis of Risk Factors and Drug Resistance of CRE Infection in Children ' s Intensive Care Unit[J]. Journal of Kunming Medical University, 2023, 44(9): 49-54.
    [4]
    吴英, 雷蕾, 刘礼初, 等. 佛山市中医院耐碳青霉烯类肠杆菌目细菌的产酶型别及耐药性分析[J]. 检验医学与临床, 2023, 20(3): 320-323. doi: 10.3969/j.issn.1672-9455.2023.03.008

    WU Y, LEI L, LIU L C, et al. Analysis of enzyme producing genotypes and drug resistance of carbapenem-resistant Enterobacteriaceae in Foshan Hospital of Traditional Chinese Medicine[J]. Laboratory Medicine and Clinic, 2023, 20(3): 320-323. doi: 10.3969/j.issn.1672-9455.2023.03.008
    [5]
    MAJUMDER M M I, MAHADI A R, AHMED T, et al. Antibiotic resistance pattern of microorganisms causing urinary tract infection: a 10-year comparative analysis in a tertiary care hospital of Bangladesh[J]. Antimicrob Resist Infect Control, 2022, 11(1): 156. doi: 10.1186/s13756-022-01197-6
    [6]
    刘玉庆. EUCAST欧盟药敏试验标准[M]. 北京: 中国质检出版社, 2016: 21-110.

    LIU Y Q. EUCAST Standard for drug susceptibility testing[M]. Beijing: China Quality Inspection Press, 2016: 21-110.
    [7]
    JONES R N, BARRY A L, THORNSBERRY C, et al. The cefoperazone-sulbactam combination. In vitro qualities including beta-lactamase stability, antimicrobial activity, and interpretive criteria for disk diffusion tests[J]. Am J ClinPathol, 1985, 84(4): 496-504.
    [8]
    KADOYAMA K, SAKAEDA T, TAMON A, et al. Adverse event profile of tigecycline: data mining of the public version of the U.S. Food and Drug Administration adverse event reporting system[J]. Biol Pharm Bull, 2012, 35(6): 967-970. doi: 10.1248/bpb.35.967
    [9]
    CLSI. Performance standards for antimicrobial susceptibility testin, 31st edition. CLSI supplement M100[EB/OL]. (2021-03-23)[2021-12-03]. Philadelphia(USA): CLSI. https://clsi.org/media/2663/m100ed29_sample.pdf.
    [10]
    陈中举, 田磊, 杨为民, 等. 2016—2018年泌尿外科患者尿路感染病原菌分布及耐药性分析[J]. 临床泌尿外科杂志, 2020, 35(2): 103-107, 111.

    CHEN Z J, TIAN L, YANG W M, et al. Analysis of pathogenic bacterium distribution and antibiotic resistance among urologic patients with urinary infection in 2016-2018[J]. Journal of Clinical Urology, 2020, 35(2): 103-107, 111.
    [11]
    张丽, 齐军, 周凯, 等. 肿瘤患者分离的耐碳青霉烯类肠杆菌目细菌耐药基因及耐药性[J]. 中华医院感染学杂志, 2022, 32(14): 2085-2089.

    ZHANG L, QI J, ZHOU K, et al. Drug resistance genes and drug resistance in carbapenem-resistant Enterobacteriaceae isolated from tumor patients[J]. Chinese Journal of Nosocomiology, 2022, 32(14): 2085-2089.
    [12]
    邓燕燕, 龚倩, 李牧, 等. 神经外科碳青霉烯类耐药肠杆菌科细菌的耐药率及耐药基因分析[J]. 检验医学与临床, 2023, 20(8): 1072-1076. doi: 10.3969/j.issn.1672-9455.2023.08.012

    DENG Y Y, GONG Q, LI M, et al. Analysis of drug resistance rate and drug resistance genes of Enterobacteria resistant to carbapenemase in neurosurgery[J]. Laboratory Medicine and Clinic, 2023, 20(8): 1072-1076. doi: 10.3969/j.issn.1672-9455.2023.08.012
    [13]
    张丽, 齐军, 周凯, 等. 肿瘤患者分离的耐碳青霉烯类肠杆菌目细菌耐药基因及耐药性[J]. 中华医院感染学杂志, 2022, 32(14): 2085-2089.

    ZHANG L, QI J, ZHOU K, et al. Drug resistance genes and drug resistance in carbapenem-resistant Enterobacteriaceae isolated from tumor patients[J]. Chinese Journal of Nosocomiology, 2022, 32(14): 2085-2089.
    [14]
    赵赟安, 黄建胜, 史杨, 等. 23株碳青霉烯耐药大肠埃希菌blaNDM基因分子流行病学[J]. 中华医院感染学杂志, 2021, 31(8): 1121-1128.

    ZHAO Y A, HUANG J S, SHI Y, et al. Molecular epidemiology of blaNDM gene in 23 strains of carbapenem-resistant Escherichia coli[J]. Chinese Journal of Nosocomiology, 2021, 31(8): 1121-1128.
    [15]
    佘亚辉, 韩媛媛, 柴杰, 等. 203株耐碳青霉烯类肠杆菌科细菌临床分布特征及耐药基因型分析[J]. 安徽医药, 2020, 24(5): 885-888. doi: 10.3969/j.issn.1009-6469.2020.05.009

    SHE Y H, HAN Y Y, CHAI J, et al. Analysis of clinical distribution and drug resistance genotyping of 203 carbapenem enterobacteriaceae strains[J]. Anhui Medical and Pharmaceutical Journal, 2020, 24(5): 885-888. doi: 10.3969/j.issn.1009-6469.2020.05.009
    [16]
    张玉敏, 顾全, 韩素桂, 等. 碳青霉烯类耐药肠杆菌科细菌耐药基因分型与临床感染分析[J]. 热带医学杂志, 2022, 22(12): 1656-1660. doi: 10.3969/j.issn.1672-3619.2022.12.010

    ZHANG Y M, GU Q, HAN S G, et al. Genotyping and clinical infection of carbapenem resistant Enterobacteriaceae[J]. Journal of Tropical Medicine, 2022, 22(12): 1656-1660. doi: 10.3969/j.issn.1672-3619.2022.12.010
    [17]
    张侃, 刘长鑫, 王博, 等. 呼吸内科耐碳青霉烯类肺炎克雷伯菌不同耐药基因型的临床特征分析[J]. 国际呼吸杂志, 2020, 40(3): 167-171.

    ZHANG K, LIU C X, WANG B, et al. Clinical characteristics of different drug-resistance genes of carbapenem resistant Klebsiella pneumoniae in department of respiratory medicine[J]. International Journal of Respiration, 2020, 40(3): 167-171.
    [18]
    KANAHASHI T, MATSUMURA Y, YAMAMOTO M, et al. Comparison of the XpertCarba-R and NG-Test CARBA5 for the detection of carbapenemases in an IMP-type carbapenemase endemic region in Japan[J]. J Infect Chemother, 2021, 27(3): 503-506. doi: 10.1016/j.jiac.2020.11.001
    [19]
    王安阁, 王爽, 刘璐, 等. 山东省潍坊市农村居民耐碳青霉烯类阴沟肠杆菌耐药基因特征及核心基因组特征分析[J]. 中华预防医学杂志, 2024, 58(1): 48-55.

    WANG A G, WANG S, LIU L, et al. Analysis of gene characteristics and core genome characteristics of carbapenem-resistant Enterobacter cloacae in rural residents of Weifang City, Shandong Province[J]. Chinese Journal of Preventive Medicine, 2024, 58(1): 48-55.
    [20]
    汪一萍, 倪剑锋, 鲁勇, 等. CRE耐药基因的流行情况与耐药情况[J]. 检验医学与临床, 2022, 19(14): 1891-1894, 1898.

    WANG Y P, NI J F, LU Y, et al. Prevalence and drug resistance situation of CRE resistance genes[J]. Laboratory Medicine and Clinic, 2022, 19(14): 1891-1894, 1898.
    [21]
    ZHANG R, SHEN Y, WAISH T R, et al. Use of polymyxins in Chinese hospitals[J]. Lancet Infect Dis, 2020, 20(10): 1125-1126.
    [22]
    EL-SAYED AHMED M A E, ZHONG L L, SHEN C, et al. Colistin and its role in the Era of antibiotic resistance: an extended review (2000-2019)[J]. Emerg Microbes Infect, 2020, 9(1): 868-885.
    [23]
    HUANG H, DONGg N, SHU L, et al. Colistin-resistance gene mcr in clinical carbapenem-resistant Enterobacteriaceae strains in China, 2014-2019[J]. Emerg Microbes Infect, 2020, 9(1): 237-245.
    [24]
    鲁勇, 汪一萍, 应建飞, 等. 产mcr-1耐碳青霉烯类大肠埃希菌的分子特征研究[J]. 现代实用医学, 2023, 35(2): 163-165, 204.

    LU Y, WANG Y P, YING J F, et al. Molecular characteristics of mcr-1 producing carbapenem-resistant Escherichia coli[J]. Modern Practical Medicine, 2023, 35(2): 163-165, 204.
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