Volume 22 Issue 11
Nov.  2024
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Article Navigation >  Chinese Journal of General Practice  > 2024  >  22(11): 1822-1826
YU Jinhui, WANG Wei, LYU Zhenyu, JI Wenbin, YU Huihao, YANG Yan. Comprehensive analysis of the prognostic and immunological significance of EXO1 in hepatocellular carcinoma[J]. Chinese Journal of General Practice, 2024, 22(11): 1822-1826. doi: 10.16766/j.cnki.issn.1674-4152.003742
Citation: YU Jinhui, WANG Wei, LYU Zhenyu, JI Wenbin, YU Huihao, YANG Yan. Comprehensive analysis of the prognostic and immunological significance of EXO1 in hepatocellular carcinoma[J]. Chinese Journal of General Practice, 2024, 22(11): 1822-1826. doi: 10.16766/j.cnki.issn.1674-4152.003742
shu

Comprehensive analysis of the prognostic and immunological significance of EXO1 in hepatocellular carcinoma

doi: 10.16766/j.cnki.issn.1674-4152.003742

  • Department of Medical Oncology, the First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui 233004, China

Funds:

 2023AH040291

 gxyq2022042

 Byycx23058

  • Received Date: 2024-05-11
    Available Online: 2024-12-31
    Abstract
  •   Objective  To analyze the expression of exonuclease 1 (EXO1) in hepatocellular carcinoma (HCC) and to investigate the relationship between EXO1 and prognosis and immune cell infiltration in patients with HCC.  Methods  The expression of EXO1 in HCC and normal tissues was analyzed based on The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) databases, Furthermore, EXO1 mRNA expression was verified in HCC cell lines by qRT-PCR analysis. The correlation between EXO1 expression and clinicopathological features was analyzed using logistic regression. Kaplan-Meier analysis and Cox regression were used to determine the correlation between EXO1 expression and patients prognosis. The GSVA ssGSEA algorithm and the TIMER database were employed to examine the correlation between EXO1 expression and the level of immune infiltration.  Results  The expression of EXO1 mRNA in HCC tissues was found to be significantly higher than that in normal tissues (P < 0.001). Furthermore, the EXO1 mRNA level in HCC cell lines, BEL-7404 and SMMC-7721 was observed to be markedly elevated in comparison to that in a normal hepatic cell line LO2 (3.92±0.75, 2.42±0.52 vs. 1.00±0.00; P < 0.05). There was a significant correlation between EXO1 expression and the T stage, histological grade and pathological stage of tumors (P < 0.001). A high level of EXO1 expression was found to be associated with poorer overall survival rate in HCC patients (P < 0.001). The analysis of immune infiltration revealed a correlation between EXO1 expression and the infiltration of multiple immune cells.  Conclusion  High EXO1 expression is associated with an unfavourable prognosis and immune cell infiltration in HCC patients. This may serve as a potential prognostic biomarker and immune intervention target in HCC.

     

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