Volume 23 Issue 2
Feb.  2025
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Article Navigation >  Chinese Journal of General Practice  > 2025  >  23(2): 207-210
BAI Wenhua, LI Xiangpei, LIU Juan, LIU Yan, JIANG Zhen. Correlations between miR-223/ mTOR/ S6K pathway and the activity of rheumatoid arthritis and antibody levels[J]. Chinese Journal of General Practice, 2025, 23(2): 207-210. doi: 10.16766/j.cnki.issn.1674-4152.003868
Citation: BAI Wenhua, LI Xiangpei, LIU Juan, LIU Yan, JIANG Zhen. Correlations between miR-223/ mTOR/ S6K pathway and the activity of rheumatoid arthritis and antibody levels[J]. Chinese Journal of General Practice, 2025, 23(2): 207-210. doi: 10.16766/j.cnki.issn.1674-4152.003868
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Correlations between miR-223/ mTOR/ S6K pathway and the activity of rheumatoid arthritis and antibody levels

doi: 10.16766/j.cnki.issn.1674-4152.003868
  • 1.

    Department of Rheumatology and Immunology, the Huaian Clinical College of Xuzhou Medical University, Huaian, Jiangsu 223300, China

Funds:

 BRA2020226

  • Received Date: 2024-05-12
    Available Online: 2025-03-27
    Abstract
  •   Objective  To investigate the expression levels of miR-223 and the mTOR / S6K pathway in synovial tissues of rheumatoid arthritis (RA) and their correlation with disease activity and antibody levels.  Methods  A total of 17 RA patients who were hospitalized in the Department of Rheumatology and Immunology of Huaian First People' s Hospital from January 2016 to December 2017 with moderate to severe disease RA were selected, along with 9 patients hospitalized for knee osteoarthritis (OA) surgery as controls. The synovial tissue from the knees of RA patients was obtained under the guidance of ultrasound, while tissue from OA patients were obtained by orthopedic surgeons. The expression levels of mTOR, S6K, RPS6 mRNAs, and miR-223 level in the synovial tissue were detected by qRT-PCR. Differences in miR-223 level and mRNA levels of mTOR, S6K, and RPS6 between the two groups were compared. The correlations between the level of miR-223 and the mRNA levels of mTOR, S6K, and RPS6, as well as anti-CCP antibody and disease activity indicators, were analyzed in the RA group.  Results  The miR-223 level in the RA group was significantly increased compared with the OA group (P < 0. 001), while the mRNA levels of S6K and RPS6 in the RA group were significantly decreased compared with the OA group (P < 0.05), There was no statistically significant difference in the expression level of mTOR mRNA between the RA group and the OA group. The miR-223 level in the RA group was negatively correlated with the mRNA levels of S6K and RPS6 (P < 0.05), but no significant correlation was found between miR-223 level and the level of mTOR mRNA. The levels of miR-223 and mTOR mRNA in the RA group were negatively correlated with the serum antiCCP antibody level (P < 0.05), while the level of RPS6 mRNA was positively correlated with the anti-CCP antibody level (P=0.009). The miR-223 level in the RA group was positively correlated with the serum CRP level (P=0.043), There was no correlation between the mRNA levels of RPS6, mTOR, and S6K and the CRP level.  Conclusion  An abnormal miR-223/ mTOR / S6K pathway exists in the synovial tissue of RA knee joints, correlating with the levels of anti-CCP antibody and CRP, indicating the potential pathways for further research into the pathogenesis of RA.

     

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