Volume 23 Issue 2
Feb.  2025
Turn off MathJax
Article Contents
Article Navigation >  Chinese Journal of General Practice  > 2025  >  23(2): 261-264
LI Ling, YANG Min, LIU Wenchun. Expression changes and clinical significance of CST1 in children with asthma[J]. Chinese Journal of General Practice, 2025, 23(2): 261-264. doi: 10.16766/j.cnki.issn.1674-4152.003881
Citation: LI Ling, YANG Min, LIU Wenchun. Expression changes and clinical significance of CST1 in children with asthma[J]. Chinese Journal of General Practice, 2025, 23(2): 261-264. doi: 10.16766/j.cnki.issn.1674-4152.003881
shu

Expression changes and clinical significance of CST1 in children with asthma

doi: 10.16766/j.cnki.issn.1674-4152.003881

  • Department of Pediatric Neurorespiratory Rehabilitation, Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi, Hubei 445000, China

Funds:

 E20200013

  • Received Date: 2024-01-19
    Available Online: 2025-03-27
    Abstract
  •   Objective  To investigate the expression of cysteine protease inhibitor SN (CST1) in peripheral blood of childhood asthma and its clinical significance.  Methods  Fifty-two asthma children (observation group) and 52 health were selected in Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi from May 2021 to September 2022. Peripheral venous blood was taken in the observation group and control group on the day after admission, and the expression of serum CST1, IFN-γ, IL-33, IL-4, and IL-13 was detected by ELISA. Spearman correlation analysis was used to analyze the correlation between serum CST1 expression and children-asthma control test (C-ACT) score, IL-33, IL-4, and IL-13 levels. The ROC curve was used to evaluate the diagnostic efficacy of CST1 expression in asthma children.  Results  Compared with the control group, the expression of CST1 protein in the serum of the observation group was significantly increased (P < 0.01). Spearman correlation analysis showed that CST1 expression was negatively correlated with C-ACT score in the observation group (r=-0.601, P < 0.001) and was negatively correlated with IFN-γ expression (r=-0.413, P=0.002). The expression of CST1 was positively correlated with the levels of serum IL-33 (r=0.416, P=0.002), IL-4 and IL-13 (r=0.542, P < 0.001; r=0.425, P=0.002). ROC curve analysis showed that the AUC of serum CST1 expression in the diagnosis of childhood asthma was 0.876 (95% CI: 0.807-0.945), and its cut-off value was 944 pg/mL, with a sensitivity of 90.4% and specificity of 61.5%. The AUC of predicting the severity of the disease by CST1 expression in serum was 0.711 (95% CI: 0.568-0.854), and the cutoff value was 1 404 pg/mL, with a sensitivity of 70.4% and a specificity of 68.0%.  Conclusion  The expression of CST1 protein in the serum of asthma children is increased, and it is correlated with C-ACT score, serum IL-33, IL-4, and IL-13. Serum CST1 expression can be used as a biological indicator for the diagnosis and severity of childhood asthma.

     

    • FullText(HTML)
  • loading
    • References(21)
  • [1]
    PORSBJERG C, MELEN E, LEHTIMAKI L, et al. Asthma[J]. Lancet, 2023, 401(10379): 858-873.
    [2]
    BRYANT N, MUEHLING L M. T-cell responses in asthma exacerbations[J]. Ann Allergy Asthma Immunol, 2022, 129(6): 709-718.
    [3]
    QIN Z, CHEN Y, WANG Y, et al. Immunometabolism in the pathogenesis of asthma[J]. Immunology, 2024, 171(1): 1-17. http://www.nstl.gov.cn/paper_detail.html?id=1a303be6e931dbcd168bd39725a0693c
    [4]
    CHATZIPARASIDIS G, BUSH A, CHATZIPARASIDI M R, et al. Airway epithelial development and function: a key player in asthma pathogenesis?[J]. Paediatr Respir Rev, 2023, 47: 51-61. doi: 10.1016/j.prrv.2023.04.005
    [5]
    YAN B, REN Y, LIU C, et al. Cystatin SN in type 2 inflammatory airway diseases[J]. J Allergy Clin Immunol, 2023, 151(5): 1191-1203. doi: 10.1016/j.jaci.2023.02.005
    [6]
    GAO X, TIAN Y, LIU Z L, et al. Tick salivary protein cystatin: structure, anti-inflammation and molecular mechanism[J]. Ticks Tick Borne Dis, 2024, 15(2): 102289. DOI: 10.1016/j.ttbdis.2023.102289.
    [7]
    WANG M, GONG L, LUO Y, et al. Transcriptomic analysis of asthma and allergic rhinitis reveals CST1 as a biomarker of unified airways[J]. Front Immunol, 2023, 14: 1048195. DOI: 10.3389/fimmu.2023.1048195.
    [8]
    中华儿科杂志编辑委员会, 中华医学会儿科学分会呼吸学组, 中国医师协会儿科医师分会儿童呼吸专业委员会. 儿童支气管哮喘规范化诊治建议(2020年版)[J]. 中华儿科杂志, 2020, 58(9): 708-717.

    Editorial Committee of Chinese Pediatrics Journal, Respiratory Group of Pediatrics Branch of Chinese Medical Association, Pediatric Respiratory Professional Committee of Pediatricians Branch of Chinese Medical Doctor Association. Recommendations for diagnosis and management of bronchial asthma in children(2020)[J]. Chinese Journal of Pediatrics, 2020, 58(9): 708-717.
    [9]
    JI T, LI H. T-helper cells and their cytokines in pathogenesis and treatment of asthma[J]. Front Immunol, 2023, 14: 1149203. DOI: 10.3389/fimmu.2023.1149203.
    [10]
    赵桐, 王怡云, 关菲, 等. 难治性哮喘患儿外周血关键基因的筛选、验证和诊断价值分析[J]. 华西医学, 2023, 38(2): 270-277.

    ZHAO T, WANG Y Y, GUAN F, et al. Bioinformatic screening, expression validation and diagnostic value analysis of key genes in peripheral blood of childhood therapy-resistant asthma[J]. West China Medical Journal, 2023, 38(2): 270-277.
    [11]
    HUANG K, YANG T, XU J, et al. Prevalence, risk factors, and management of asthma in China: a national cross-sectional study[J]. Lancet, 2019, 394(10196): 407-418. http://www.xueshufan.com/publication/2949174534
    [12]
    CARAMORI G, NUCERA F, MUMBY S, et al. Corticosteroid resistance in asthma: cellular and molecular mechanisms[J]. Mol Aspects Med, 2022, 85: 100969. DOI: 10.1016/j.mam.2021.100969.
    [13]
    GBD 2019 Chronic Respiratory Diseases Collaborators. Global burden of chronic respiratory diseases and risk factors, 1990-2019: an update from the global burden of disease study 2019[J]. E Clin Med, 2023, 59: 101936. DOI: 10.1016/j.eclinm.2023.101936.
    [14]
    RABY K L, MICHAELOUDES C, TONKIN J, et al. Mechanisms of airway epithelial injury and abnormal repair in asthma and COPD[J]. Front Immunol, 2023, 14: 1201658. DOI: 10.3389/fimmu.2023.1201658.
    [15]
    黄月, 魏小松, 江超, 等. 类风湿关节炎患者血清KL-6和IL-33的表达水平及其临床意义[J]. 中华全科医学, 2023, 21(11): 1856-1859, 1950. doi: 10.16766/j.cnki.issn.1674-4152.003242

    HUANG Y, WEI X S, JIANG C, et al. Expression levels of serum KL-6 and IL-33 in patients with rheumatoid arthritis and their clinical significance[J]. Chinese Journal of General Practice, 2023, 21(11): 1856-1859, 1950. doi: 10.16766/j.cnki.issn.1674-4152.003242
    [16]
    GAUVREAU G M, BERGERON C, BOULET L P, et al. Sounding the alarmins-the role of alarmin cytokines in asthma[J]. Allergy, 2023, 78(2): 402-417. doi: 10.1111/all.15609
    [17]
    NOCERA A L, MUELLER S K, WORKMAN A D, et al. Cystatin SN is a potent upstream initiator of epithelial-derived type 2 inflammation in chronic rhinosinusitis[J]. J Allergy Clin Immunol, 2022, 150(4): 872-881. doi: 10.1016/j.jaci.2022.04.034
    [18]
    SINGHANIA A, WALLINGTON J C, SMITH C G, et al. Multitissue transcriptomics delineates the diversity of airway T cell functions in asthma[J]. Am J Respir Cell Mol Biol, 2018, 58(2): 261-270. doi: 10.1165/rcmb.2017-0162OC
    [19]
    YAN Z, LIU L, JIAO L, et al. Bioinformatics analysis and identification of underlying biomarkers potentially linking allergic rhinitis and asthma[J]. Med Sci Monit, 2020, 26: e924934. DOI: 10.12659/MSM.924934.
    [20]
    DU L, XU C, TANG K, et al. Epithelial CST1 promotes airway eosinophilic inflammation in asthma via the AKT signaling pathway[J]. Allergy Asthma Immunol Res, 2023, 15(3): 374-394. doi: 10.4168/aair.2023.15.3.374
    [21]
    DIVER S, SRIDHAR S, KHALFAOUI L C, et al. Feno differentiates epithelial gene expression clusters: exploratory analysis from the mesos randomized controlled trial[J]. J Allergy Clin Immunol, 2022, 150(4): 830-840. doi: 10.1016/j.jaci.2022.04.024
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(1)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (15) PDF downloads(1) Cited by()
    Proportional views
    Related

    Address：No. 287, Changhuai Road, Bengbu, Anhui Province, 233004, P.R.ChinaphoneNo：0552-3066635； 0552-3051890Email：zhqkyx@163.com

    Copyright © Chinese Journal of General Practice皖ICP备2020018345号-2

    Supported by: Beijing Renhe Information Technology Co., Ltd.    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return