Volume 23 Issue 3
Mar.  2025
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Article Navigation >  Chinese Journal of General Practice  > 2025  >  23(3): 383-387
ZHANG Liya, ZHU Pan, JIN Xiamin, JIE Qingqing, CUI Yingbo, CHEN Lili. Differential expression of circular RNAs in the plasma of premature infants with retinopathy[J]. Chinese Journal of General Practice, 2025, 23(3): 383-387. doi: 10.16766/j.cnki.issn.1674-4152.003909
Citation: ZHANG Liya, ZHU Pan, JIN Xiamin, JIE Qingqing, CUI Yingbo, CHEN Lili. Differential expression of circular RNAs in the plasma of premature infants with retinopathy[J]. Chinese Journal of General Practice, 2025, 23(3): 383-387. doi: 10.16766/j.cnki.issn.1674-4152.003909
shu

Differential expression of circular RNAs in the plasma of premature infants with retinopathy

doi: 10.16766/j.cnki.issn.1674-4152.003909

  • Newborn Center, Women and Children's Hospital of Ningbo University, Ningbo, Zhejiang 315012, China

Funds:

 2021KY319

 2021KY324

 2019A21002

 2023Y21

 2022-B17

 2022020405

  • Received Date: 2024-03-21
    Available Online: 2025-05-14
    Abstract
  •   Objective  This study aims to analyze the expression profile changes of circular RNA (circRNA) in the plasma of children with retinopathy of prematurity (ROP).  Methods  Three children with ROP hospitalized at Women and Children's Hospital of Ningbo University from January 2023 to August 2023 were selected as the ROP group, while three children without ROP during the same period were selected as the control group. High-throughput sequencing technology was used to detect and screen for differentially expressed circRNAs in hemorrhagic plasma, followed by bioinformatic analysis including gene ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Online databases were employed to predict the potential target microRNAs (miRNAs) of circRNA.  Results  Compared with the control group, a total of 108 circRNAs were significantly differentially expressed in the plasma of patients in the ROP group (P<0.05, fold change≥1), with 41 upregulated and 67 downregulated circRNAs. Bioinformatics analysis revealed that pathways such as proton-transporting ATP synthase and cellular metabolism play important roles in the occurrence and development of ROP. The relationship between differentially expressed circRNA and miRNA was predicted using miRanda and psRobot software, and a circRNA-miRNA regulatory network diagram was constructed.  Conclusion  Differential expression of circRNA exists between the ROP group and the control group, suggesting their potential involvement in the occurrence and development of ROP, or as novel molecular markers for diagnosis and treatment of ROP.

     

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