Volume 23 Issue 4
Apr.  2025
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Article Navigation >  Chinese Journal of General Practice  > 2025  >  23(4): 618-622
SHEN Nan, QIAO Jibing, JIANG Yazhou, WU Rang, YU Qiao, ZHU Suyue. Analysis of changes in gut microbiota structure in neonatal hyperbilirubinemia caused by early-onset sepsis[J]. Chinese Journal of General Practice, 2025, 23(4): 618-622. doi: 10.16766/j.cnki.issn.1674-4152.003964
Citation: SHEN Nan, QIAO Jibing, JIANG Yazhou, WU Rang, YU Qiao, ZHU Suyue. Analysis of changes in gut microbiota structure in neonatal hyperbilirubinemia caused by early-onset sepsis[J]. Chinese Journal of General Practice, 2025, 23(4): 618-622. doi: 10.16766/j.cnki.issn.1674-4152.003964
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Analysis of changes in gut microbiota structure in neonatal hyperbilirubinemia caused by early-onset sepsis

doi: 10.16766/j.cnki.issn.1674-4152.003964

  • Department of Pediatrics, Suqian Hospital Affiliated to Xuzhou Medical University, Suqian, Jiangsu 223800, China

Funds:

 F202153

  • Received Date: 2024-04-10
    Available Online: 2025-06-30
    Abstract
  •   Objective  The utilization of high-throughput sequencing technology facilitates the elucidation of the gut microbiota structure and the alterations in neonatal hyperbilirubinemia (NH) occasioned by early onset-sepsis (EOS).  Methods  A total of 25 children diagnosed with NH caused by EOS in Suqian Hospital Affiliated to Xuzhou Medical University from December 2022 to July 2023, and 26 healthy newborns born during the same period were included in the study. The NH group and the control group were defined, respectively. All newborn stool samples were collected and DNA was extracted. 16S rDNA amplicon sequencing was performed after passing the quality inspection. The Shannon index and ace index, which are used to assess diversity, were employed to compare the richness and diversity between the two groups. The structural similarity of microbiota was compared using the weighted unifrac distance in the β diversity analysis. To identify significant differences in bacteria at multiple levels, the linear discriminant analysis (LDA) and LDA Effect Size (LEfSe) analyses were used. Finally, the richness of the top ten strains in the two groups was compared and the biomarkers were screened.  Results  A total of 281 operational taxonomic units (OTUs) were obtained in the two groups, including 157 in the NH group, 124 in the control group, and the same 66 OTUs. The Shannon and Ace indices exhibited an increase in the NH group, with the Shannon index demonstrating statistical significance (P < 0.05). The key bacterial species (biomarkers) in the NH group are characterized by a decrease in the richness of Streptococcus salivarius (P < 0.05), and an increase in the richness of Escherichia (P < 0.05), Klebsiella pneumoniae (P < 0.05), and Streptococcus oralis (P < 0.05).  Conclusion  The diversity of the gut microbiota has been observed to increase in newborns with NH caused by EOS. At the phylum and genus levels, the most significant changes in NH group abundance were observed to be Firmicutes and Escherichia. The shift in key species was characterized by a decrease in beneficial bacteria and an increase in opportunistic pathogenic bacteria.

     

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    • References(20)
  • [1]
    QATTEA I, FARGHALY M A A, ELGENDY M, et al. Neonatal hyperbilirubinemia and bilirubin neurotoxicity in hospitalized neonates: analysis of the US database[J]. Pediatr Res, 2022, 91(7): 1662-1668. doi: 10.1038/s41390-021-01692-3
    [2]
    BRIGGS-STEINBERG C, ROTH P. Early-onset sepsis in newborns[J]. Pediatr Rev, 2023, 44(1): 14-22. doi: 10.1542/pir.2020-001164
    [3]
    王洁洁, 王国凯. 茵栀黄口服液联合双歧杆菌四联活菌治疗新生儿黄疸的效果分析[J]. 中华全科医学, 2022, 20(7): 1158-1161. doi: 10.16766/j.cnki.issn.1674-4152.002547

    WANG J J, WANG G K. Analysis of the curative effect of of Yinzhihuang oral liquid combined with bifidobacterium tetragenous viable bacteria in the treatment of neonatal jaundice[J]. Chinese Journal of General Practice, 2022, 20(7): 1158-1161. doi: 10.16766/j.cnki.issn.1674-4152.002547
    [4]
    TSAI M L, LIN W Y, CHEN Y T, et al. Adjuvant probiotic Bifidobacterium animalis subsp. lactis CP-9 improve phototherapeutic treatment outcomes in neonatal jaundice among full-term newborns: a randomized double-blind clinical study[J]. Medicine(Baltimore), 2022, 101(45): e31030. DOI: 10.1097/MD.0000000000031030.
    [5]
    CHEN K W, YUAN T M. The role of microbiota in neonatal hyperbilirubinemia[J]. Am J Transl Res, 2020, 12(11): 7459-7474.
    [6]
    KEMPER A R, NEWMAN T B, SLAUGHTER J L, et al. Clinical practice guideline revision: management of hyperbilirubinemia in the newborn infant 35 or more weeks of gestation[J]. Pediatrics, 2022, 150(3): e2022058859. DOI: 10.1542/peds.2022-058859.
    [7]
    中华医学会儿科学分会新生儿学组, 中国医师协会新生儿科医师分会感染专业委员会. 新生儿败血症诊断及治疗专家共识(2019年版)[J]. 中华儿科杂志, 2019, 57(4): 252-257.

    Subspecialty Group of Neonatology, the Society of Pediatric, Chinese Medical Association, Professional Committee of Infectious Diseases, Neonatology Society, Chinese Medical Doctor Association. Expert consensus on the diagnosis and management of neonatal sepsis (version 2019)[J]. Chinese Journal of Pediatrics, 2019, 57(4): 252-257.
    [8]
    DING J, MA X, HAN L P, et al. Gut microbial alterations in neonatal jaundice pre-and post-treatment[J]. Biosci Rep, 2021, 41(4): BSR20210362. DOI: 10.1042/BSR20210362.
    [9]
    霍晨达. 基于16s rRNA高通量测序技术对恶性梗阻性黄疸围手术期肠道菌群多样性改变的研究[D]. 太原: 山西医科大学, 2023.

    HUO C D. Research of the gut microbiota diversity in patients with malignant obstructive jaundice during the perioperative period based on 16S rRNA sequencing[D]. Taiyuan: Shanxi Medical University, 2023.
    [10]
    贾尔肯阿依·阿不都拉依, 寇新红, 陈凤娟, 等. 迟发型母乳性黄疸患儿肠道菌群特征研究[J]. 中国妇幼卫生杂志, 2022, 13(1): 57-61.

    JIAERKENAYI A, KOU X H, CHEN F J, et al. Characteristics of intestinal flora in children with late onset breast milk jaundice[J]. Chinese Journal of Women and Children Health, 2022, 13(1): 57-61.
    [11]
    赵力锐, 徐艳, 叶黎离, 等. 未足月胎膜早破早产儿早发败血症肠道菌群的特征及临床意义[J]. 徐州医科大学学报, 2021, 41(4): 289-294.

    ZHAO L R, XU Y, YE L L, et al. Characteristics and clinical significance of intestinal microbiota in early-onset sepsis of pretern infants with premature rupture of membranes[J]. Journal of Xuzhou Medical University, 2021, 41(4): 289-294.
    [12]
    BROWNE H P, SHAO Y, LAWLEY T D. Mother-infant transmission of human microbiota[J]. Curr Opin Microbiol, 2022, 69: 102173. DOI: 10.1016/j.mib.2022.102173.
    [13]
    陶丽梅, 姜春明. 肠道菌群和黄疸的研究进展[J]. 中国微生态学杂志, 2021, 33(1): 112-115.

    TAO L M, JIANG C M. Progress in research on intestinal flora and jaundice[J]. Chinese Journal of Microecology, 2021, 33(1): 112-115.
    [14]
    MUTLU M, ASLAN Y, KADER S, et al. Preventive effects of probiotic supplementation on neonatal hyperbilirubinemia caused by isoimmunization[J]. Am J Perinatol, 2020, 37(11): 1173-1176.
    [15]
    CHOUDHARY P, KRAATZ H B, LEVESQUE C M, et al. Microencapsulation of Probiotic Streptococcus salivarius LAB813[J]. ACS Omega, 2023, 8(13): 12011-12018.
    [16]
    LAO J, GUÉDON G, LACROIX T, et al. Abundance, diversity and role of ICEs and IMEs in the adaptation of Streptococcus salivarius to the environment[J]. Genes(Basel), 2020, 11(9): 999. DOI: 10.3390/genes11090999.
    [17]
    KOMSANI M R, ALMAGHLOUTH N K, CHARLA S, et al. Escherichia coli meningitis in a 72-year-old woman[J]. R I Med J, 2024, 107(1): 12-14.
    [18]
    CUI M M, SUN W, XUE Y, et al. Hepatitis E virus and Klebsiella pneumoniae co-infection detected by metagenomics next-generation sequencing in a patient with central nervous system and bloodstream Infection: a case report[J]. BMC Infect Dis, 2024, 24(1): 33. DOI: 10.1186/s12879-023-08850-4.
    [19]
    唐炜, 卢红艳, 孙勤, 等. 高胆红素血症新生儿肠道菌群特点及与β-葡萄糖醛酸苷酶活性的相关性[J]. 中国当代儿科杂志, 2021, 23(7): 677-683.

    TANG W, LU H Y, SUN Q, et al. Characteristics of gut microbiota and its association with the activity of β-glucuronidase in neonates with hyperbilirubinemia[J]. Chinese Journal of Contemporary Pediatrics, 2021, 23(7): 677-683.
    [20]
    ELMASSRY M M, KIM S, BUSBY B. Predicting drug-metagenome interactions: variation in the microbial β-glucuronidase level in the human gut metagenomes[J]. PLoS One, 2021, 16(1): e0244876. DOI: 10.1371/journal.pone.0244876.
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