Volume 23 Issue 6
Jun.  2025
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Article Navigation >  Chinese Journal of General Practice  > 2025  >  23(6): 937-941
ZHANG Zhongchuan, XU Zhiyuan, XU Wendi, ZHANG Jingquan, YUAN Lingli. Experimental study on the promotion of bone defect healing by GelMa hydrogel loaded with Puerarin composite scaffold[J]. Chinese Journal of General Practice, 2025, 23(6): 937-941. doi: 10.16766/j.cnki.issn.1674-4152.004037
Citation: ZHANG Zhongchuan, XU Zhiyuan, XU Wendi, ZHANG Jingquan, YUAN Lingli. Experimental study on the promotion of bone defect healing by GelMa hydrogel loaded with Puerarin composite scaffold[J]. Chinese Journal of General Practice, 2025, 23(6): 937-941. doi: 10.16766/j.cnki.issn.1674-4152.004037
shu

Experimental study on the promotion of bone defect healing by GelMa hydrogel loaded with Puerarin composite scaffold

doi: 10.16766/j.cnki.issn.1674-4152.004037
  • 1.

    Department of Orthopedics, the Second Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui 233040, China

Funds:

 KJ2021A0757

 KJ2021A0756

 AHWJ2023A20575

  • Received Date: 2024-12-23
    Available Online: 2025-09-04
    Abstract
  •   Objective  To prepare a new kind of biomaterial that can promote the healing of bone defect by constructing the GelMa composite hydrogel loaded with Puerarin.  Methods  Scanning electron microscope and Young's modulus were used to analyze the physical characteristics of the composite scaffolds. Gradient concentrations of Puerarin/GelMa scaffolds (20, 40, 80, and 100 μmol/L) were constructed, and the effects of scaffolds on cell proliferation at different time points were investigated by CCK-8. ALP and alizarin red staining were used to investigate the effect of various stents on osteogenic differentiation and select the most suitable stents. A craniotomy defect model was successfully established. Employing a completely randomized design, rats were randomly allocated into four groups by means of a random number table. These four groups were designated as the blank group, the GelMa group, the puerarin group, and the puerarin + GelMa group. Each group consisted of three rats, resulting in a total of twelve rats for the study. Samples were collected 8 weeks after surgery. micro-CT, Western Blotting, HE staining, Masson's staining, and ALP immunohistochemical staining were performed on the skull samples to evaluate the effect of Puerarin+GelMa scaffolding on bone defects in vivo.  Results  The porosity and pore size of the composite scaffold were similar to that of GelMa. Young's modulus showed no significant difference between the composite scaffold [(3.30±0.30) MPa] and the single GelMa [(3.22±0.25) MPa, t=0.359, P=0.738]. CCK-8 experiment showed that Puerarin had the strongest promotion effect on osteoblast proliferation at 80 μmol/L (P < 0.05). ALP and alizarin red staining showed that the positive area percentage of the 80 μmol/L group was the largest (P < 0.05). micro-CT, Western Blotting, HE staining, Masson's staining, and ALP immunohistochemistry showed that the Puerarin+GelMa group had the most new bone tissue growth, and its repair ability for bone defects was significantly higher than that of the blank group (P < 0.05).  Conclusion  Puerarin+GelMa scaffold with a puerarin concentration of 80 μmol/L has the ability to promote osteogenic differentiation of osteoblasts, and can effectively promote bone defect healing in vivo, which can be used as a new biomaterial for bone defect repair.

     

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