The expression of astrocyte elevated gene-1(AEG-1) in cervical cancer and it's correlation with angiogenesis

Objective To observe the expression of astrocyte increased gene 1(AEG-1) in cervical cancer and it’s correlation with microvessel density,vascular endothelial growth factor(NF-κB) and nuclear factor κB(p65 VEGF). Methods Forty-five cases of invasive cervical carcinoma and 12 cases of chronic cervicitis were collected from the First Affiliated Hospital of WenZhou Medical University.The expression levels of AEG-1,p65 VEGF and NF-κB protein were detected by immunohistochemical MaxVision method,and the microvessel density(MVD) was detected by tumor microvessel endothelial markers(CD34) and Weidner count method.The expression of AEG-1,p65 NF-κB and VEGF in cervical cancer tissues was observed under optical microscope and the staining was observed.Correlation of AEG-1 expression with p65 NF-κB and VEGF expression in cervical cancer tissues was analyzed by Pearson. Results The expression of AEG-1 in cervical cancer and chronic cervicitis were (0.186±0.043) and (0.051±0.002),and the difference was statistically significant(P<0.01).AEG-1 expression level was positively correlated with lymph node metastasis of cervical cancer(P<0.01),while it was not positively correlated with the indexes of age,differentiation,tumor size,pathological type,and adjacent to the peri-uterus organizations(P>0.05).AEG-1 expression and p65 NF-κB(r=0.501,P＜0.001),VEGF(r=0.718,P＜0.001) and MVD(r=0.815，P＜0.001) were positively correlated with Pearson. Conclusions AEG-1 is highly expressed in cervical cancer tissues,and it can promote angiogenesis of cervical cancer.The mechanism may be related to AEG-1 activation of NF-κB signaling pathway and the regulation of VEGF levels.