Construction and detection of metastases in animal models

The metastasis and recurrence of tumor are the important reasons leading to the death of patients who suffer from carcinomas.There are more and more therapeutic methods of tumors with the development of medical science.However,it still lacks methods of preventing tumors.So,it requires structuring animal tumor models and tumor metastasis models to simulate the process of tumor metastasis in human body.Analyzing domestic and overseas literature reports retrospectively,contrasting characteristics of various species animals and cell lines and analyzing methods which are used in structuring common metastatic tumor models.After structuring animal models successfully,we choose corresponding technological means according to the object of detection.The rodents are commonly used to structure metastatic tumor models,which mainly include nude mouse and rats that have clear genetic backgrounds.The choice of experiment cells depends on the requirement of experiment.We choose different characteristics of cells when structuring different tumor metastasis models.The methods of cell inoculation divide into orthotopic inoculation and ectopic inoculation according to different inoculation positions.Transplants divide into cell suspension and tissue blocks.The evaluations of experiment results require detections of tumor in situ and metastasis tumor at the same time.The detection methods divide into traditional detection modes and modern technological detection modes.When the detection objects are different,we can choose different modern technological detection mode to combine with traditional detection.In order to acquire the best ideal evaluation results of metastasis tumor models,we should combine various methods of detection and evaluation.Structuring experiment animal models can simulate the occurrence,development and metastasis of tumor in human body.Various animals can be used to structure metastasis tumor animal models and the rodent animals which have clear genetic backgrounds are the most common experiment animals.The structure of metastasis tumor animal models requires us to understand the details in every process.Whether the structure of models is successful,we can evaluate in many aspects by traditional detection methods and modern technology.