Abstract: Objective To explore the best method for chloral hydrate sedation for cases like cranial computer tomography (CT) and brainstem auditory evoked potential. Methods The 398 newborns who needed chloral hydrate sedation were randomly divided into three groups according to three different dosing methods,namely mouth feeding for 98 infants,rectal administration for 150 cases,and tube feeding for the rest 150 neonates.There were no significant differences between weights at birth,ages in days or kinds of diseases for the infants in the three groups.To observe the smoothness of operations for the three groups,then monitor the sedative effects and occurance of adverse reactions within 24 hours for those infants who successfully took medicine in. Results The difference between the degree of ease of the three methods was statistically significant(P<0.05).When comparing the smoothness of operations for the groups one by one,there was significant difference between tube feeding group and rectal administration group(P<0.012 5),and there was significant difference between tube feeding group and mouth feeding group(P<0.012 5).However,there was no significant difference between the degree of ease between rectal administration group and mouth feeding group(P>0.012 5).The sedative effects of the three groups showed no significant difference (P>0.05).Within 24 hours,the only significant difference in adverse reactions had been the possibility of getting diarrhea (P<0.05).Comparing the possibility of getting diarrhea for groups one by one,there was significant difference between rectal administration group and tube feeding group (P<0.012 5).The difference was also significant between rectal administration group and mouth feeding group (P<0.012 5).There was no significant difference in the possibility of getting diarrhea between tube feeding group and mouth feeding group(P>0.012 5). Conclusion As for neonatal sedation,tube feeding is better than mouth feeding and rectal administration,since it smooths the operation with lower pain level for patient and less adverse reactions.It reduces the workload for nurses as well.