Abstract: Objective To explore the effect of multi-round multi-module grading training method in laparoscopic visual reality surgical skill training in participants with different clinical experiences. Methods The training was divided into different round and module.There were basic module (the first module),middle level module(the second module) and advanced module(the third module) in each round.The training started from the first module of the first round training.After a certain hours(6 hours) of training,the assessments should be taken,the doctors who pass the exam came into a higher level module training in the same round,the doctors who failed to enter the next module still learning in the corresponding module of the second round until they pass the exam and then came into the next module in the same round.So,if it is still no successful promotion in the second round,then the doctors continued to practice in the corresponding module of the next round.The training completed after passing three modules for certain round and receiving the final review by the surgical experts. Results Total 55 doctors participated in 3 rounds training.The training started from the first module of the first round.Every doctor accepted the assessment after a 6 hours training.28 trainees passed the examination and then entered into the second module of the first round training.After another 6 hours training,16 trainees passed the examination and entered into the third module of the first round training.16 trainees passed the three modules of the first round training.The qualified students will joint in the final expert review at last.After the same training way at the second and the third round of training,all the 55 doctors passed the training.After the multi-round multi-module grading skill training in laparoscopic visual reality operation exercises,the sense of space position and operation handle feel,skillful using of instruments and the ability of both hand-eye and hand-hand had improved significantly of the doctors with different clinical experiences.More than 70% of the passing students can transit to clinical practice gradually. Conclusion In the laparoscopic visual reality skill training,application of the method of multi-round multi-module grading training,the laparoscopic operation skills of the students with different clinical experience improved obviously,and laid a solid foundation for the students for the approaching clinical practice.